A5047927545- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Lipid Membrane Structure and Behavior
- Virus-based gene therapy research
- Graphene and Nanomaterials Applications
- RNA regulation and disease
- Surfactants and Colloidal Systems
- Electrostatics and Colloid Interactions
- Animal Virus Infections Studies
- Cellular transport and secretion
- Spectroscopy and Quantum Chemical Studies
- Nanotechnology research and applications
- Bacteriophages and microbial interactions
- Monoclonal and Polyclonal Antibodies Research
- DNA and Nucleic Acid Chemistry
- Chemical Synthesis and Analysis
- Advanced Drug Delivery Systems
- MicroRNA in disease regulation
- Bacterial Genetics and Biotechnology
- SARS-CoV-2 and COVID-19 Research
- HER2/EGFR in Cancer Research
- Mitochondrial Function and Pathology
University of Dubai
2023-2024
University of Brighton
2021-2024
King's College London
2007-2023
University College London
2009
Recent research in the field of nonviral gene delivery vectors has focused on preparing nanoparticles that are stabilized by incorporation a PEG coating and where one vector components is also cleavable. Here,we describe synthesis, formulation, transfection properties, biophysical studies PEG-stabilized ternary lipopolyplex which, for first time, both lipid peptide designed to be cleaved once been internalized. A series cationic lipids, bearing short tri- or hexaethylene glycol groups,...
The roll-out of the Pfizer-BioNTech BNT162b2 COVID-19 vaccine has brought many logistical challenges, such as absence comprehensive stability data leading to strict handling instructions during dilution and administration. Accidental mishandling therefore presents challenging clinical dilemmas, which often led providers err on side caution discard mishandled vials rather than risk administering ineffective vaccine. This study aims answer key questions about vaccine's allow for a more...
Cationic peptide sequences, whether linear, branched, or dendritic, are widely used to condense and protect DNA in both polyplex lipopolyplex gene delivery vectors. How these peptides behave within particles the consequences this has on transfection efficiency remain poorly understood. We have compared, parallel, a complete series of cationic peptides, branched coformulated with plasmid give polyplexes, lipid, DOTMA, mixed 50% neutral helper DOPE, lipopolyplexes, correlated efficiencies...
Lipoplexes (LDs) have been prepared from DNA, DOPE and either a dichain oxyethylated cationic lipid or their novel trichain (TC) counterpart.
Ternary nanocomplexes, composed of bifunctional cationic peptides, lipids and siRNA, as delivery vehicles for siRNA have been investigated.
The structure, biophysical properties and biological behavior of lipopolyplex ternary gene delivery vectors incorporating novel C14 glycerol based lipids varying alkyl chain geometry (containing cis, trans or alkyne double bonds) have been studied in the presence absence a bifunctional targeting peptide designed to both condense DNA confer integrin-specific targeting. In vitro transfection studies breast cancer MDA-MB-231 cells revealed that formulations lipid:peptide:DNA (LPD) complexes...
Lipopolyplexes (LPDs) are of considerable interest for use as gene delivery vehicles. Here LPDs have been prepared from cationic vesicles (composed a 1 : molar ratio DOTMA with the neutral helper lipid, DOPE), singly branched peptides and plasmid DNA. All contained linker sequence (cleaved by endosomal furin) attached to targeting selected bind human airway epithelial cells mediate delivery. The current study investigates effects novel Arg-containing peptide sequences on biophysical...
Cationic amphipathic pH responsive peptides possess high in vitro and vivo nucleic acid delivery capabilities function by forming a non-covalent complex with cargo, protecting it from nucleases, facilitating uptake via endocytosis responding to endosomal acidification being released the inserting into disordering membranes. We have designed synthesised show how Coulombic interactions between ionizable 2,3-diaminopropionic (Dap) side chains can be manipulated tune functional response of...
The formation of a novel trichain (TC) lipid was discovered when cationic possessing terminal hydroxyl group and the helper dioleoyl l-α-phosphatidylethanolamine (DOPE) were formulated as vesicles stored. Importantly, transfection efficacies lipopolyplexes comprised TC lipid, targeting peptide DNA (LPDs) found to be higher than corresponding dichain (DC) used. To explore this interesting discovery determine if concept can more generally applied improve gene delivery efficiencies, design...
The effects have been determined of a systematic alteration the alkyl chain geometry C14 analogue DOTMA on detailed molecular architecture resulting cationic vesicles formed both in absence and presence 50 mol% DOPE, lipoplexes prepared from these using either calf thymus or plasmid DNA. analogues studied involved cis- trans-double bonds at positions Δ9 Δ11, compound (ALK) featuring an alkyne position C9. For all analogues, examination by light scattering neutron scattering, zeta potential...
Among the known breast cancers, subtype with HER2 receptors-overexpressing cells is associated a poor prognosis. The adopted monoclonal antibody Trastuzumab has improved clinical outcomes, but it drug resistance and relatively high costs. present work peptide solid-phase synthesis method to synthesise branched poly(ε-lysine) dendrons 8 branching arms integrating, at their carboxy terminal molecular root, either an arginine or receptor-binding sequence LSYCCK scramble CSCLYK. These were...