A5045535688- Antimicrobial Peptides and Activities
- RNA Interference and Gene Delivery
- Lipid Membrane Structure and Behavior
- Advanced biosensing and bioanalysis techniques
- Biochemical and Structural Characterization
- Gut microbiota and health
- Bacteriophages and microbial interactions
- Gastrointestinal motility and disorders
- Advanced NMR Techniques and Applications
- Antibiotic Resistance in Bacteria
- Photoreceptor and optogenetics research
- Probiotics and Fermented Foods
- Inhalation and Respiratory Drug Delivery
- Aquaculture disease management and microbiota
- Reproductive tract infections research
- DNA and Nucleic Acid Chemistry
- Solid-state spectroscopy and crystallography
- Clostridium difficile and Clostridium perfringens research
- Nanoparticle-Based Drug Delivery
- Virus-based gene therapy research
- Protein Hydrolysis and Bioactive Peptides
- Cystic Fibrosis Research Advances
- Nicotinic Acetylcholine Receptors Study
- Polydiacetylene-based materials and applications
- Geology and Paleoclimatology Research
King's College London
2016-2025
Pharmaceutical Biotechnology (Czechia)
2021
University of Oxford
1999-2020
Centre National de la Recherche Scientifique
2005-2017
Université de Strasbourg
2009-2017
Institut de Chimie de Strasbourg
2009-2017
Institut de Chimie
2005-2010
Inserm
2008
Hôpitaux Universitaires de Strasbourg
2008
Goethe University Frankfurt
2004-2005
The present study describes leucine zipper peptide–lipid hybrid nanoscale vesicles engineered by self-assembled anchoring of the amphiphilic peptide within lipid bilayer. These aim to combine advantages traditional temperature-sensitive liposomes (TSL) with dissociative, unfolding properties a optimize drug release under mild hyperthermia, while improving in vivo retention. secondary structure and its thermal responsiveness after onto were studied circular dichroism. In addition,...
Soils in permafrost regions contain twice as much carbon the atmosphere, and has an important influence on natural built environment at high northern latitudes. The response of to warming climate is uncertain occurs time scales longer than those assessed by direct observation. We dated periods speleothem growth a north-south transect caves Siberia reconstruct history past states. Speleothem restricted full interglacial conditions all studied caves. In northernmost cave (at 60°N), no occurred...
Perivascular adipose tissue (PVAT) plays a vital role in maintaining vascular homeostasis. However, most studies ascribed the function of PVAT remodeling to adipokines secreted by perivascular adipocytes. Whether mesenchymal stem cells exist and play regeneration remain unknown. Approach Results: Single-cell RNA-sequencing allowed direct visualization heterogeneous PVAT-derived (PV-ADSCs) at high resolution revealed 2 distinct subpopulations, among which one featured signaling pathways...
The interaction of antimicrobial peptides (AMPs) with the inner membrane Gram-negative bacteria is a key determinant their abilities to exert diverse bactericidal effects. Here we present molecular level understanding initial target for two cationic α-helical AMPs that share structural similarities but have ten-fold difference in antibacterial potency towards bacteria. binding and insertion from solution pleurocidin or magainin 2 membranes representing bacteria, comprising mixture 128...
INTRODUCTION: The low FODMAP diet (LFD) reduces symptoms and bifidobacteria in irritable bowel syndrome (IBS). β-galactooligosaccharides (B-GOS) may reduce the increase IBS. We investigated whether B-GOS supplementation alongside LFD improves IBS while preventing decline bifidobacteria. METHODS: performed a randomized, placebo-controlled, 3-arm trial of 69 Rome III adult patients with from secondary care United Kingdom. Patients were randomized to sham placebo supplement (control) or...
The syndrome of spontaneous preterm birth (sPTB) presents a challenge to mechanistic understanding, effective risk stratification, and clinical management. Individual associations between sPTB, self-reported ethnic ancestry, vaginal microbiota, metabolome, innate immune response are known but not fully understood, knowledge has yet impact practice. Here, we used multi–data type integration composite statistical models gain insight into sPTB by exploring the cervicovaginal environment an...
The histidine-rich amphipathic cationic peptide LAH4 has antibiotic and DNA delivery capabilities. Here, we explore the interaction of peptides from this family with model membranes as monitored by solid-state (2)H nuclear magnetic resonance their activities against a range bacteria. At neutral pH, membrane disruption is weak, but at acidic strongly disturb anionic lipid component bacterial cause lysis. are effective antibiotics both pH 7.2 5.5, although antibacterial activity affected...
Designed histidine-rich amphipathic cationic peptides, such as LAH4, have enhanced membrane disruption and antibiotic properties when the peptide adopts an alignment parallel to surface. Although this was previously achieved by lowering pH, here we designed a new generation of peptides that adopt surface at neutral pH. In vitro, are powerful antibiotics in terms concentrations required for activity; spectrum target bacteria, fungi, parasites; speed with which they kill. Further modifications...
Despite the fundamental contribution of gut microbiota to host physiology, extent its variation in genetically-identical animals used research is not known. We report significant divergence both composition and metabolism adult C57BL/6 mice housed separate controlled units within a single commercial production facility. The reported has potential confound experimental studies using mammalian models.
Pulmonary delivery of siRNA has considerable therapeutic potential for treating viral respiratory infectious diseases including influenza. By introducing that targets the conserved region genes encoding nucleocapsid protein (NP), mRNAs can be degraded and replication inhibited in mammalian cells. To enable to used as an antiviral agent, nucleic acid barrier must overcome. Effective local lung tissues is required reduce dose minimize systemic adverse effects. develop a formulation suited...
The major intracellular barriers associated with DNA delivery using nonviral vectors are inefficient endosomal/lysosomal escape and poor nuclear uptake. LAH4-L1, a pH responsive cationic amphipathic peptide, is an efficient vector that promotes the release of nucleic acid into cytoplasm through endosomal escape. Here we further enhance transfection efficiency LAH4-L1 by incorporating localizing signal (NLS) to promote importation. Four NLSs were investigated: Simian virus 40 (SV40) large...
The histidine‐rich designer peptides of the LAH4 family exhibit potent antimicrobial, transfection, transduction and cell‐penetrating properties. They form non‐covalent complexes with their cargo, which often carry a negative overall charge at pH 7.4 include large range molecules structures such as oligonucleotides, including siRNA DNA, peptides, proteins, nanodots adeno‐associated viruses. These are thought to enter cells through an endosomal pathway where acidification organelle is...
Chronic disruption of the intestinal microbiota in adult cystic fibrosis (CF) patients is associated with local and systemic inflammation, has been linked to risk serious comorbidities. Supplementation high amylose maize starch (HAMS) might provide clinical benefit by promoting commensal bacteria biosynthesis immunomodulatory metabolites. However, whether disrupted CF gut capacity utilise these substrates not known. We combined metagenomic sequencing, vitro fermentation, amplicon...
Summary Background The low FODMAP diet (LFD) leads to clinical response in 50%–80% of patients with irritable bowel syndrome (IBS). It is unclear why only some respond. Aims To determine if differences baseline faecal microbiota or and urine metabolite profiles may separate responders the from non‐responders allowing predictive algorithms be proposed. Methods We recruited adults fulfilling Rome III criteria for IBS a blinded randomised controlled trial. Patients were sham placebo supplement...
Abstract Antimicrobial resistance (AMR) is a major cause of death worldwide, with 1.27 M direct deaths from bacterial drug-resistant infections as 2019. Dissemination multidrug-resistant (MDR) bacteria in the environment, conjunction pharmapollution by active pharmaceutical ingredients (APIs), create and foster an environmental reservoir AMR. Creative solutions are required to mitigate AMR, while taking into consideration other aspects planetary “Triple Crisis” pollution, biodiversity loss,...