A5070052895- Iron Metabolism and Disorders
- Erythropoietin and Anemia Treatment
- Hemoglobinopathies and Related Disorders
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- Proteoglycans and glycosaminoglycans research
- Erythrocyte Function and Pathophysiology
- Pregnancy and preeclampsia studies
- Trace Elements in Health
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
Radboud University Nijmegen
2018-2022
Radboud University Medical Center
2018-2022
Radboud Institute for Molecular Life Sciences
2021-2022
University Medical Center
2022
Azienda Ospedaliera di Padova
2021
University of Padua
2021
Walter de Gruyter (Germany)
2021
Abstract Background Hepcidin concentrations measured by various methods differ considerably, complicating interpretation. Here, a previously identified plasma-based candidate secondary reference material (csRM) was modified into serum-based two-leveled sRM. We validated its functionality to increase the equivalence between for international standardization. Methods applied technical procedures developed International Consortium Harmonization of Clinical Laboratory Results. The sRM,...
Abstract Objectives Hepcidin measurement advances insights in pathophysiology, diagnosis, and treatment of iron disorders, but requires analytically sound standardized procedures (MPs). Recent development a two-level secondary reference material (sRM) for hepcidin assays allows worldwide standardization. However, no proficiency testing (PT) schemes to ensure external quality assurance (EQA) exist the absence high calibrator sRM set precludes optimal Methods We developed pilot PT together...
Hemolysis is known to cause acute kidney injury (AKI). The iron regulatory hormone hepcidin, produced by renal distal tubules, suggested exert a renoprotective role during this pathology. We aimed elucidate the molecular mechanisms of hepcidin synthesis and its protection against hemoglobin-induced AKI. In contrast hepatic induction, incubation mouse cortical collecting duct (mCCDcl1) cells with IL-6 or LPS did not induce Hamp1 mRNA expression, whereas (FeS) hemin significantly induced (p <...
Syndecan-1 (Sdc-1) is a heparan sulfate proteoglycan that can bind cytokines and chemokines via its side chains, has immunomodulatory properties in experimental models. Sdc-1 expression been reported on dendritic cells (DC) T cells. The potential role of DC—T cell interaction not investigated yet. We postulate involved DC–T may influence graft survival an allogeneic transplant model. bone marrow-derived DC was analyzed by flow cytometry. Unstimulated LPS stimulated deficient were evaluated...
Peptide hormone hepcidin regulates systemic iron metabolism and has been described to be partially bound α2-macroglobulin albumin in blood. However, the reported degree of protein binding varies between <3% ≈89%. Since protein-binding may influence function quantification, better insight into is essential fully understand biological behavior interpretation its measurement patients. Here, we used peritoneal dialysis assess human a functional setting for first time. We measured freely...
Erythroferrone (ERFE), the erythroid regulator of iron metabolism, inhibits hepcidin to increase availability for erythropoiesis. ERFE plays a pathological role during ineffective erythropoiesis as occurs in X-linked sideroblastic anemia (XLSA) and β-thalassemia. Its measurement might serve an indicator severity these diseases. However, reliable quantification analytical characterization is indispensable determine assay's limitations define proper methodology. We developed sandwich ELISA...
Abstract Syndecan-1 (Sdc-1) is a heparan sulfate proteoglycan that can bind cytokines and chemokines via its side chains, has immunomodulatory properties in experimental models. Sdc-1 expression been reported on dendritic cells (DC) T cells. The potential role of DC - cell interaction not investigated yet. We postulate involved – may influence graft survival an allogeneic transplant model. bone marrow-derived was analyzed by flow cytometry. Unstimulated LPS stimulated deficient were...
The dendritic cell (DC)-derived cytokine profile contributes to naive T differentiation, thereby directing the immune response. IL-37 is a with anti-inflammatory characteristics that has been demonstrated induce tolerogenic properties in DC. In this study we aimed evaluate influence of on DC-T interaction, special focus role chemokine CXCL1. DC were cultured from bone marrow human transgenic (hIL-37Tg) or WT mice. phenotype unstimulated and LPS-stimulated was analyzed (co-stimulatory...
Plasma metanephrines and prospective prediction of tumor location, size