A5080020086- Renal Diseases and Glomerulopathies
- Proteoglycans and glycosaminoglycans research
- Systemic Lupus Erythematosus Research
- Platelet Disorders and Treatments
- Chronic Kidney Disease and Diabetes
- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- Glycosylation and Glycoproteins Research
- Complement system in diseases
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Fibroblast Growth Factor Research
- Phagocytosis and Immune Regulation
- Epigenetics and DNA Methylation
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Renal and related cancers
- Genomics and Chromatin Dynamics
- Immune responses and vaccinations
- Immune cells in cancer
- Cancer-related gene regulation
- Diabetes and associated disorders
- Vasculitis and related conditions
- Nitric Oxide and Endothelin Effects
Radboud University Nijmegen
2015-2024
Radboud University Medical Center
2015-2024
Radboud Institute for Molecular Life Sciences
2015-2024
University Medical Center
2014-2023
RELX Group (United States)
2018
University of Bristol
2017
Friedrich-Alexander-Universität Erlangen-Nürnberg
2017
Pediatric Nephrology of Alabama
2005-2016
Bipar
2013
University Medical Center Groningen
2006-2007
Regeneration of injured tubular cells occurs after acute necrosis primarily from intrinsic renal cells. This may occur a pre-existing intratubular stem/progenitor cell population or any surviving proximal cell. In this study, we characterize CD24-, CD133-, and vimentin-positive subpopulation scattered throughout the tubule in normal human kidney. Compared to adjacent 'normal' cells, these CD24-positive contained less cytoplasm, fewer mitochondria, no brush border. addition, 49 marker...
Abstract Sialic acid sugars on the surface of cancer cells have emerged as potent immune modulators that contribute to immunosuppressive microenvironment and tumor evasion. However, mechanisms by which these modulate antitumor immunity well therapeutic strategies directed against them are limited. Here we report intratumoral injections with a sialic mimetic Ac53FaxNeu5Ac block expression in vivo suppress growth multiple models. blockade had major impact cell composition tumor, enhancing...
An excessive release and impaired degradation of neutrophil extracellular traps (NETs) leads to the continuous exposure NETs endothelium in a variety hematologic autoimmune disorders, including lupus nephritis. This study aims unravel mechanisms through which jeopardize vascular integrity.Microvascular macrovascular endothelial cells were exposed NETs, subsequent effects on integrity function determined vitro vivo. We found that have limited capacity internalize via receptor for advanced...
The release of neutrophil extracellular traps (NETs), either during "suicidal" or "vital" NETosis, represents an important strategy neutrophils to combat Gram-negative bacteria. Lipopolysaccharide (LPS), a major component the outer membrane bacteria, is reported stimulus for NET formation. Although it widely acknowledged that structural diversity in LPS structures can elicit heterogeneous immune responses, species- and serotype-specific differences capacity trigger formation have not yet...
ABSTRACTIn Drosophila melanogaster, the Polycomb-group (PcG) and trithorax-group (trxG) genes have been identified as repressors activators, respectively, of gene expression. Both groups are required for stable transmission expression patterns to progeny cells throughout development. Several lines evidence suggest a functional interaction between PcG trxG proteins. For example, genetic indicates that enhancer zeste [E(z)] can be considered both trxGgene. To better understand molecular...
Polycomb (Pc) is part of a Pc group (PcG) protein complex that involved in repression gene activity during Drosophilaand vertebrate development. To identify proteins interact with homologs, we performed two-hybrid screens withXenopus (XPc) and human 2 (HPC2). We find the C-terminal binding (CtBP) interacts XPc HPC2, CtBP HPC2 coimmunoprecipitate, partially colocalize large PcG domains interphase nuclei. unknown function binds to conserved 6-amino-acid motif C terminus adenovirus E1A protein....
AbstractThe Polycomb (Pc) protein is a component of multimeric, chromatin-associated group (PcG) complex, which involved in stable repression gene activity. The identities components the PcG complex are largely unknown. In two-hybrid screen with vertebrate Pc homolog as target, we identify human RING1 interacting Pc. that contains RING finger motif, specific zinc-binding domain, found many regulatory proteins. So far, function has remained enigmatic. Here, show coimmunoprecipitates homolog,...
Changes in chromatin structure are a key aspect the epigenetic regulation of gene expression. We have used lac operator array system to visualize by light microscopy effect heterochromatin protein 1 (HP1) α (HP1α) and HP1β on large-scale living mammalian cells. The HP1, containing chromodomain, chromoshadow domain, hinge allows it bind variety proteins. In vivo targeting an enhanced green fluorescent protein-tagged HP1-lac repressor fusion operator-containing, gene-amplified chromosome...
about 15% to 30% of the DNA in human sperm is packed nucleosomes and transmission this fraction embryo potentially serves as a mechanism facilitate paternal epigenetic programs during embryonic development. However, hitherto it has not been established whether these are removed like protamines or indeed contribute zygotic chromatin, thereby contributing epigenome embryo.to clarify fate sperm-derived we have used deposition characteristics histone H3 variants from which follows that...
Diabetic nephropathy (DN) is the major life-threatening complication of diabetes. Abnormal permselectivity glomerular basement membrane (GBM) plays an important role in DN pathogenesis. Heparanase predominant enzyme that degrades heparan sulfate (HS), main polysaccharide GBM. Loss GBM HS diabetic kidney was associated with increased expression heparanase; however, causal involvement heparanase pathogenesis has not been demonstrated. We report for first time essential DN. With use Hpse-KO...
Changes in endothelial glycocalyx are one of the earliest changes development cardiovascular disease. The is both an important biological modifier interactions between flowing blood and vessel wall, a determinant organ perfusion. We hypothesize that deeper penetration erythrocytes into associated with reduced microvascular population-based prospective cohort study (the Netherlands Epidemiology Obesity [NEO] study) includes 6,673 middle-aged individuals (oversampling overweight obese...
Objective Circulating chromatin‐containing apoptotic material and/or neutrophil extracellular traps (NETs) have been proposed to be an important driving force for the antichromatin autoimmune response in patients with systemic lupus erythematosus (SLE). The aim of this study was determine exact nature microparticles circulation SLE and assess effects on immune system. Methods We analyzed isolated from plasma SLE, rheumatoid arthritis (RA), sclerosis (SSc), as well healthy subjects....