A5037625247- Proteoglycans and glycosaminoglycans research
- Renal Diseases and Glomerulopathies
- Acute Lymphoblastic Leukemia research
- Fibroblast Growth Factor Research
- Cell Adhesion Molecules Research
- Biochemical and Molecular Research
- Platelet Disorders and Treatments
- Glycosylation and Glycoproteins Research
- Chronic Kidney Disease and Diabetes
- Advanced Glycation End Products research
- Clostridium difficile and Clostridium perfringens research
- Protein Tyrosine Phosphatases
- Complement system in diseases
- Diabetes and associated disorders
- Chronic Lymphocytic Leukemia Research
- T-cell and Retrovirus Studies
- Metabolism, Diabetes, and Cancer
- Adolescent and Pediatric Healthcare
- Cardiovascular Disease and Adiposity
- Glaucoma and retinal disorders
- Cytomegalovirus and herpesvirus research
- Antibiotic Resistance in Bacteria
- Systemic Sclerosis and Related Diseases
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Lymphatic System and Diseases
Radboud University Nijmegen
2000-2019
Radboud University Medical Center
1986-2019
Radboud Institute for Molecular Life Sciences
2009-2017
University Medical Center
2016
University Medical Center Groningen
2007
Howard Hughes Medical Institute
2005
University of Iowa
2005
Max Planck Institute for Developmental Biology
1998
Erasmus University Rotterdam
1981-1983
Objective Circulating chromatin‐containing apoptotic material and/or neutrophil extracellular traps (NETs) have been proposed to be an important driving force for the antichromatin autoimmune response in patients with systemic lupus erythematosus (SLE). The aim of this study was determine exact nature microparticles circulation SLE and assess effects on immune system. Methods We analyzed isolated from plasma SLE, rheumatoid arthritis (RA), sclerosis (SSc), as well healthy subjects....
Heparan sulfate, the polysaccharide side chain of heparan sulfate proteoglycan, is important for permselective properties glomerular basement membrane. In this report, we show a role hydroxyl radicals in degradation and an enhanced membrane permeability. First, enzyme-linked immunosorbent assay, exposure coated (proteoglycan) to reactive oxygen species resulted ±50% decrease binding monoclonal antibody against whereas core protein remained unaltered. Second, on polyacrylamide gel...
The effects of complete human intestinal flora and anaerobes on germ‐free mice were studied. gross composition the was similar to that with which animals had been contaminated appeared be stable provided kept isolated. reduced caecal weight normal values induced an antagonistic effect against Escherichia coli. In contrast flora, not invasive in immunosuppressed colonization resistance antagonism Pseudomonas aeruginosa.
Persistent exposure of the immune system to death cell debris leads autoantibodies against chromatin in patients with systemic lupus erythematosus (SLE). Deposition anti-chromatin/chromatin complexes can instigate inflammation multiple organs including kidney. Previously we identified specific death-associated histone modifications as targets SLE. In this study addressed, a large cohort SLE and controls, question whether plasma reactivities peptides associated serology clinical features....
We determined the specificity of two hamster monoclonal antibodies and a sheep polyclonal antiserum against heparan sulfate proteoglycan isolated from rat glomerular basement membrane. The were characterized by enzyme-linked immunosorbent assay on various membrane components immunoprecipitation with or without heparitinase pre-treatment. These experiments showed that specifically recognize approximately 150-, 105-, 70-kDa core proteins proteoglycan. Recently, we agrin is major in (Groffen,...
We raised monoclonal antibodies (MAb) against the core protein and heparan sulfate (HS) side chain of proteoglycan (HSPG) from glomerular basement membranes (GBM). Anti-HSPG-core MAb were obtained after immunization mice with HSPG purified human GBM anti-HS rat glomeruli, which crossreacted HS HSPG. The specificity was demonstrated by ELISA studies, Western blotting, inhibition experiments, indirect immunofluorescence (IF) on kidney cryostat sections pre-treated glycosaminoglycan...
Obese women have higher risk of bearing breast tumors that are highly aggressive and resistant to therapies. Tumor-promoting effects obesity occur locally via adipose inflammation related alterations the extracellular matrix (ECM) as well systemically circulating metabolic mediators (e.g., free fatty acids, FFA) associated with excess adiposity implicated in toll-like receptor-mediated activation macrophages-key cellular players obesity-related cancer progression. Although contribution...
In a time-study of active Heymann nephritis, the expression agrin, main heparan sulfate proteoglycan in glomerular basement membrane, was analyzed relation to deposition IgG and complement capillary wall development albuminuria. Binding autoantibodies could be detected from 2 wk onward, followed by activation after 6 wk. Progressive albuminuria developed onward level 274+/-68 mg/18 h at week 12. The staining intensity for agrin core protein decreased slightly, stubs that were still attached...