A5042954849- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Prostate Cancer Treatment and Research
- Clinical Laboratory Practices and Quality Control
- Virus-based gene therapy research
- Oral microbiology and periodontitis research
- Cancer Genomics and Diagnostics
- Biosimilars and Bioanalytical Methods
- Prostate Cancer Diagnosis and Treatment
- Toxoplasma gondii Research Studies
- Statistical Methods in Clinical Trials
- Molecular Biology Techniques and Applications
- RNA regulation and disease
- Lung Cancer Research Studies
- Oral and gingival health research
- COVID-19 Clinical Research Studies
- Lung Cancer Diagnosis and Treatment
- Salivary Gland Disorders and Functions
- Hormonal and reproductive studies
- Biosensors and Analytical Detection
- Environmental DNA in Biodiversity Studies
- Parvovirus B19 Infection Studies
- Cancer Cells and Metastasis
- PARP inhibition in cancer therapy
- NF-κB Signaling Pathways
AC2T Research (Austria)
2021
Azienda Ospedaliera di Padova
2021
University of Padua
2021
Walter de Gruyter (Germany)
2021
Comenius University Bratislava
2016-2019
Alpha Medical (Slovakia)
2016
MRC University of Glasgow Centre for Virus Research
2011
Medical Research Council
2011
University of Glasgow
2009-2010
University of Birmingham
2008
Human cytomegalovirus (HCMV) in clinical material cannot replicate efficiently vitro until it has adapted by mutation. Consequently, wild-type HCMV differ fundamentally from the passaged strains used for research. To generate a genetically intact source of HCMV, we cloned strain Merlin into self-excising BAC. The BAC clone had mutations RL13 gene and UL128 locus that were acquired during limited replication prior to cloning. complete complement was reconstructed reference original sample....
Deep sequencing was used to bring high resolution the human cytomegalovirus (HCMV) transcriptome at stage when infectious virion production is under way, and major findings were confirmed by extensive experimentation using conventional techniques. The majority (65.1%) of polyadenylated viral RNA transcription committed producing four noncoding transcripts (RNA2.7, RNA1.2, RNA4.9, RNA5.0) that do not substantially overlap designated protein-coding regions. Additional RNAs are transcribed...
Mutations that occurred during adaptation of human cytomegalovirus to cell culture were monitored by isolating four strains from clinical samples, passaging them in various types and sequencing ten complete virus genomes the final passages. Mutational dynamics assessed targeted intermediate passages original samples. Gene RL13 UL128 locus (UL128L, consisting genes UL128, UL130 UL131A) mutated all strains. fibroblast, epithelial endothelial cells, whereas those UL128L limited fibroblasts...
We have assessed two approaches to sequencing complete human cytomegalovirus (HCMV) genomes (236 kbp) in DNA extracted from infected cell cultures (strains 3157, HAN13, HAN20 and HAN38) or clinical specimens JP 3301). The first approach involved amplifying the samples as overlapping PCR products, these by Sanger method, acquiring reads a capillary instrument assembling using Staden programs. second generating sequence data an Illumina Genome Analyzer (IGA), processing filtered...
The genomes of commonly used variants human cytomegalovirus (HCMV) strains Towne and AD169 each contain a substantial mutation in which region (U(L)/b') at the right end long unique has been replaced by an inverted duplication from left genome. Using high-throughput technology, we have sequenced HCMV strain (ATCC VR-977) confirmed presence two variants, one exhibiting replacement U(L)/b' other intact this region. Both are mutated genes RL13, UL1, UL40, UL130, US1 US9. We also novel variant...
Factors which increase the risk of severe adult periodontitis (AP) may also contribute to success dental implants. To determine cytokines be relevant, levels interleukin-1alpha (IL-1alpha), interleukin-1beta (IL-1beta), interleukin-1 receptor antagonist (IL-1ra), interleukin-6 (IL-6) and interferon-gamma (IFN-gamma) mRNA were quantitated in gingival tissue from patients healthy controls. Periodontitis significantly increased IL-1alpha, IL-1beta, IL-6 IFN-gamma relative tissues. IL-1 was...
At initial diagnosis, most patients with small-cell lung cancer (SCLC) present metastatic disease a high number of tumor cells (CTCs) circulating in the blood. We analyzed RNA transcripts specific for neuroendocrine and epithelial cell lineages, Notch pathway delta-like 3 ligand (DLL3), actionable target rovalpituzumab tesirine (Rova-T) CTC samples. Peripheral blood samples from 48 SCLC were processed using microfluidic Parsortix™ technology to enrich CTCs. Blood 26 healthy donors same way...
Abstract Objectives Quality management for clinical laboratories requires the establishment of internal procedures including standard operating (SOPs), quality control (QC), validation test results and assessment. External assessment (EQA) alternativeassessment (AAPs) are part hierarchy required diagnostic testing. The International Organization Standardization (ISO) document with requirements conformance ISO 15189 Clinical Laboratory Standards Institute (CLSI) QMS24 require participation in...
Periodontal attachment loss is mediated by overproduction of tumour necrosis factor (TNF) and interleukin (IL)‐1, appears to have a genetic component. The 8.1 major histocompatibility complex (MHC) ancestral haplotype (HLA‐A1,B8,TNFA‐308(2),DR3) associated with elevated TNF production predisposes carriers several autoimmune/immunopathological disorders, including rapid progression HIV disease, but not early onset periodontal disease in healthy individuals. Rather high proportion subjects...
The International Organization for Standardization (ISO) 15189 standard provides recommendations the postexamination reporting phase to enhance quality in clinical laboratories. purpose of this study was encourage a broad discussion on current practices molecular diagnostic tests by conducting global survey such practices.The Federation Clinical Chemistry and Laboratory Medicine's Committee Molecular Diagnostics (IFCC C-MD) surveyed laboratories selected ISO topics. addressed following...
The study aimed to assess long-term outcomes in patients with very high-risk prostate cancer (PCa) - pT3b-T4 N0-1 using the definitive histopathology following radical retropubic prostatectomy (RRP).We have analyzed 114 PCa who underwent RRP between 1995 and 2012. Biochemical clinical progression-free survival (BPFS, CPFS), cancer-specific overall (CSS, OS) curves were constructed according Kaplan-Meier method. Univariate multivariate Cox regression analysis was utilized determine...
Background/Aim: The aim of this study was to analyse the genetic profiles metastatic castration-resistant prostate cancer (mCRPC) by using next generation sequencing identify variants with pathogenic potential in nine DNA repair genes – BRCA1, BRCA2, RAD50, RAD51, RAD51C/D, ATM and ATR. Materials Methods: Isolated genomic from peripheral blood 50 patients mCRPC used for 111 associated hereditary on an Illumina platform. Identified were tested Integrative Genomic Viewer, their clinical...
Abstract A viable virus could not be recovered from a mutant murine cytomegalovirus (MCMV) BAC in which the M34 ORF had been deleted (BACΔM34). In contrast, an (RcM34), was interrupted by transposon insertion at nt 44,827 of Smith MCMV BAC, attenuated replication both tissue culture and SCID mice. Similarly, Rc3′ΔM34, 3′‐end 44,724 to 45,647, produced similar kinetics RcM34 while BAC5′ΔM34, 5′‐end 43,083 up 44,896 deleted, non‐viable like BACΔM34. transcript analysis wt virus‐infected cells...
Plasma metanephrines and prospective prediction of tumor location, size