A5009847146- Curcumin's Biomedical Applications
- Genomics, phytochemicals, and oxidative stress
- Pomegranate: compositions and health benefits
- Carcinogens and Genotoxicity Assessment
- Retinoids in leukemia and cellular processes
- Estrogen and related hormone effects
- Pharmacological Effects of Medicinal Plants
- Antioxidant Activity and Oxidative Stress
- Phytochemicals and Antioxidant Activities
- Bioactive Compounds and Antitumor Agents
- Phytochemicals and Medicinal Plants
- Nutrition, Genetics, and Disease
- Tea Polyphenols and Effects
- Free Radicals and Antioxidants
- DNA and Nucleic Acid Chemistry
- Alcohol Consumption and Health Effects
- MicroRNA in disease regulation
- Plant-Derived Bioactive Compounds
- Bioactive Compounds in Plants
- Glutathione Transferases and Polymorphisms
- DNA Repair Mechanisms
- Diet and metabolism studies
- Tannin, Tannase and Anticancer Activities
- Gastrointestinal motility and disorders
- Plant chemical constituents analysis
University of Louisville
2011-2023
James Graham Brown Foundation
2006-2017
Texas A&M University
2017
Institute of Molecular Biology and Biophysics
2011
GTx (United States)
2011
Baxter (United States)
2011
Fox Chase Cancer Center
2010
University of Kentucky
2003-2010
Universidade de São Paulo
2010
University of Louisville Hospital
2007-2008
Berries are gaining increasing importance lately for their chemopreventive and therapeutic potential against several cancers. In earlier studies, a blueberry-supplemented diet has shown protection 17β-estradiol (E2)-mediated mammary tumorigenesis. This study tested both preventive activities of supplemented with whole blueberry powder (50:50 blend Tifblue Rubel). Animals received 5% diet, either 2 weeks prior to or 12 after E2 treatment in groups, respectively. Both interventions delayed the...
Emerging research evidence has established the critical role of gut-liver axis in development alcohol-associated liver disease (ALD). The present study employed 16S rRNA gene and whole genome shotgun (WGS) metagenomic analysis combination with a revised microbial dataset to comprehensively detail butyrate-producing communities associated butyrate metabolic pathways affected by chronic ethanol feeding. Specifically, data demonstrated that decrease several bacterial genera belonging distinct...
Alcoholic liver disease (ALD) is a major cause of liver-related mortality. There still no US Food and Drug Administration-approved therapy for ALD, therefore, identifying therapeutic targets needed. Our previous work demonstrated that ethanol exposure leads to up-regulation cAMP-degrading phosphodiesterase 4 (PDE4) expression, which compromises normal cAMP signaling in monocytes/macrophages hepatocytes. This effect on contributes dysregulated inflammatory response altered lipid metabolism....
DNA damage is a pre-requisite for the initiation of cancer and agents that reduce this are useful in prevention. In study, we evaluated ability whole berries berry phytochemical, ellagic acid to endogenous oxidative damage. Ellagic was selected based on >95% inhibition 8-oxodeoxyguosine (8-oxodG) other unidentified adducts induced by 4-hydroxy-17ss-estradiol CuCl(2) vitro. Inhibition latter occurred at lower concentrations (10 microM) than 8-oxodG (100 microM). vivo female CD-1 mice (n=6)...
Abstract Lung cancer is the leading cause of deaths in United States, surpassing breast as primary cancer-related mortality women. The goal present study was to identify early molecular changes lung induced by exposure tobacco smoke and thus potential targets for chemoprevention. Female A/J mice were exposed either or HEPA-filtered air via a whole-body chamber (6 h/d, 5 d/wk 3, 8, 20 weeks). Gene expression profiles tissue from control smoke-exposed animals established using 15K cDNA...
We previously demonstrated the protective effects of blueberry (BB) and black raspberry (BRB) supplemented at 2.5% dose in an ACI rat mammary tumor model. Here, we assessed a dose-related alteration indices with diet 5% BB or BRB powder. The was well tolerated. Tumor palpation from 12 weeks revealed first appearance by 84 days control group, that delayed 24 39 diets, respectively (p = 0.04). Ellagic acid detected plasma rats fed range 96.6-294.2 ng/mL. While showed better efficacy reducing...
Ethanol-mediated down-regulation of carnitine palmitoyltransferase-1 (CPT-1A) gene expression plays a major role in the development hepatic steatosis; however, underlying mechanisms are not completely elucidated. Tributyrin, butyrate prodrug that can inhibit histone deacetylase (HDAC) activity, attenuates steatosis and injury. The present study examined beneficial effect tributyrin/butyrate attenuating ethanol-induced pathogenic epigenetic affecting CPT-1A promoter–histone modifications...
Cumulative kidney toxicity associated with cisplatin is severe and there no clear consensus on the therapeutic management of same. The pathogenesis involves activation inflammatory apoptotic pathways; therefore, regulating these pathways offers protection. Given anti-inflammatory antioxidant effects urolithin A, a gut microbial metabolite ellagic acid, our aim was to explore potential use A in prevention cisplatin-induced nephrotoxicity an experimental rat model. For this purpose, animals...
Many chemopreventive agents have encountered bioavailability issues in pre-clinical/clinical studies despite high oral doses. We report here a new concept utilizing polycaprolactone implants embedded with test compounds to obtain controlled systemic delivery, circumventing and reducing the total administered dose. Compounds were released from vitro dose dependently for long durations (months), which correlated vivo release. Polymeric of curcumin significantly inhibited tissue DNA adducts...
Dietary polyphenols may contribute to the prevention of several degenerative diseases, including cancer. Anthocyanins have been shown possess potential anticancer activity. The aim this study was determine anthocyanin bioavailability in lung tissue mice fed a blueberry diet (5% w/w) for 10 days or bolus dose (10 mg/mouse; po) native mixture bilberry anthocyanidins. All five anthocyanidins present were detected using improved methods. effect various solvents on stability anthocyanins and...
There are literally thousands of known agents with potential chemopreventive and antioxidant activity; however, the expanding list natural synthetic compounds makes it difficult to test every agent in widely accepted 2-year animal bioassay human clinical trials. Therefore, short-term screening assays needed sort out most efficacious for long-term studies. In present study, identification was explored a Cu2+-mediated Fenton-type reaction, coupled oxidative DNA lesion detection by...
Animal models play a major role in understanding the etiology, molecular mechanisms, strategizing intervention and treatment of human diseases. ACI, an inbred line derived from August Copenhagen strains, is unique for its susceptibility to estrogen-induced mammary tumors. Histologically many aspects, tumors formed these rats are similar breast cancers. Previous studies have shown high mortality significant weight loss this model associated with pituitary gland abnormality. We hypothesized...
Androgen ablation therapy is the primary therapeutic option for locally advanced and metastatic castration‐resistant prostate cancer (CRPC). We investigated effect of a dietary metabolite Urolithin A (UroA) dissected molecular mechanism in CRPC cells. Treatment with UroA inhibited cell proliferation both androgen receptor‐positive (AR + ) (C4‐2B) receptor‐negative − (PC‐3) cells however, AR CaP were more sensitive to treatment as compared Inhibition signaling was responsible on Ectopic...
Purpose: Chemokine-driven leukocyte infiltration and sustained inflammation contribute to alcohol-associated liver disease (ALD). Elevated hepatic CCL2 expression, seen in ALD, is associated with severity. However, mechanisms of regulation are not completely elucidated. Post-translational modifications (PTMs) proteins, particularly acetylation, modulate gene expression. This study examined the acetylation changes promoter-associated histone-H3 key transcription factor-NFκB regulating...
Experimental and epidemiological data associate the exposure of estrogens to cancer development in several tissues, particularly, breast, endometrium, liver, kidney. One plausible mechanism estrogen-mediated carcinogenicity is DNA damage by redox cycling estrogen catechols. Reports have shown that metabolism results 2- 4-hydroxylation catechol metabolites which can then cycle. We examined capacity endogenous estrogen, 17β-estradiol, two equine formulate a significant proportion hormone...