A5022569332- Glutathione Transferases and Polymorphisms
- Phosphodiesterase function and regulation
- Genomics, phytochemicals, and oxidative stress
- Lung Cancer Treatments and Mutations
- Cancer, Lipids, and Metabolism
- Inflammatory mediators and NSAID effects
- Metabolism, Diabetes, and Cancer
- Peroxisome Proliferator-Activated Receptors
- Estrogen and related hormone effects
- Head and Neck Cancer Studies
- Cancer Immunotherapy and Biomarkers
- Genetic factors in colorectal cancer
- Carcinogens and Genotoxicity Assessment
- Cancer, Hypoxia, and Metabolism
- Peptidase Inhibition and Analysis
- Lung Cancer Research Studies
- Helicobacter pylori-related gastroenterology studies
- Nutrition, Genetics, and Disease
- Gene expression and cancer classification
- Cancer-related molecular mechanisms research
- Colorectal Cancer Treatments and Studies
- Cancer-related Molecular Pathways
- Colorectal Cancer Screening and Detection
- CRISPR and Genetic Engineering
- Synthesis and biological activity
Fox Chase Cancer Center
2015-2024
The Wistar Institute
2016
USA Mitchell Cancer Institute
2013
University of Alabama at Birmingham
2013
University of Montevallo
2013
Southern Research Institute
2013
University of South Alabama
2013
German University in Cairo
2013
National Cancer Institute
2004-2010
University of Louisville
2006-2010
Aflatoxin B1 (AFB1) has been postulated to be a hepatocarcinogen in humans, possibly by causing p53 mutations at codon 249. AFB1 is metabolized via the phase I and II detoxification pathways; hence, genetic variation those loci may predict susceptibility effects of AFB1. To test this hypothesis, two genes, epoxide hydrolase (EPHX) glutathione S-transferase M1 (GSTM1), was contrasted with presence serum AFB1-albumin adducts, hepatocellular carcinoma (HCC), 249 mutations. Mutant alleles both...
Susceptibility to lung cancer may in part be attributable inter-individual variability metabolic activation or detoxification of tobacco carcinogens. The glutathione S-transferase M1 (GSTM1) genetic polymorphism has been extensively studied this context; two recent meta-analyses case-control studies suggested an association between GSTM1 deletion and cancer. At least 15 have published after these overviews. We undertook a new meta-analysis summarize the results 43 including >18 000...
Tumor cell resistance to alkylating agents was studied by examining Walker 256 rat mammary carcinoma cells differentially sensitive nitrogen mustards. A resistant subpopulation (WR) selected exposure chlorambucil. WR showed approximately a 15-fold the cytotoxic effects of mustards and elevated glutathione S-transferase (GST) activity when compared parent line (WS). To extend these findings, GSTs from WS were purified affinity chromatography on S-hexylglutathione coupled epoxy-activated...
Abstract Nonsteroidal anti-inflammatory drugs (NSAID) display promising antineoplastic activity for colorectal and other cancers, but toxicity from COX inhibition limits their long-term use chemoprevention. Previous studies have concluded that the basis tumor cell growth inhibitory does not require inhibition, although underlying mechanism is poorly understood. Here, we report NSAID sulindac sulfide inhibits cyclic guanosine 3′,5′-monophosphate phosphodiesterase (cGMP PDE) to increase...
Abstract CYP1A1 is involved in the metabolism of benzopyrene, a suspected lung carcinogen; it therefore conceivable that genetically determined variations its activity modify individual susceptibility to cancer. The role MspI polymorphism cancer has been widely studied but not fully clarified. We have included 2,451 cases and 3,358 controls pooled analysis 22 case‐control studies on risk. found clear association between homozygous restriction fragment length (RFLP) risk Caucasians (age‐...
This study evaluated the possible protective effects of lyophilized açaí pulp (AP) in a colitis-associated carcinogenesis (CAC) rat model and modifying effect cyanidin 3-rutinoside (C3R) on motility RKO colon adenocarcinoma cells, using wound healing assay. Male Wistar rats were induced to develop CAC 1,2-dimethylhydrazine (DMH) 2,4,6-trinitrobenzene acid (TNBS). Animals randomly assigned different groups that received basal diet or supplemented with 5.0% 7.5% AP. The findings indicate: 1)...
Prolonged exposure to mutagenic substances is strongly associated with an individual's risk of developing colorectal cancer. Clinical investigation oltipraz as a chemopreventive agent supported by its induction the expression detoxication enzymes in various tissues, and protective activity against formation chemically induced tumors animals. The goals present study were: determine if could induce detoxicating gene human tissues; identify effective non-toxic doses for more extensive clinical...
DNA damage is a pre-requisite for the initiation of cancer and agents that reduce this are useful in prevention. In study, we evaluated ability whole berries berry phytochemical, ellagic acid to endogenous oxidative damage. Ellagic was selected based on >95% inhibition 8-oxodeoxyguosine (8-oxodG) other unidentified adducts induced by 4-hydroxy-17ss-estradiol CuCl(2) vitro. Inhibition latter occurred at lower concentrations (10 microM) than 8-oxodG (100 microM). vivo female CD-1 mice (n=6)...
BackgroundThe impact of the antiinflammatory agent 5-aminosalicylic acid (5-ASA) on risk for colitis-associated colorectal cancer remains controversial. The chemopreventive activity 5-ASA was evaluated in Swiss Webster model azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced neoplasia.
Abstract Lung cancer is the leading cause of deaths in United States, surpassing breast as primary cancer-related mortality women. The goal present study was to identify early molecular changes lung induced by exposure tobacco smoke and thus potential targets for chemoprevention. Female A/J mice were exposed either or HEPA-filtered air via a whole-body chamber (6 h/d, 5 d/wk 3, 8, 20 weeks). Gene expression profiles tissue from control smoke-exposed animals established using 15K cDNA...
Abstract We hypothesized that cells bearing a single inherited “hit” in tumor suppressor gene express an altered mRNA repertoire may identify targets for measures could delay or even prevent progression to carcinoma. report here on the transcriptomes of primary breast and ovarian epithelial cultured from BRCA1 BRCA2 mutation carriers controls. Our comparison analyses identified multiple changes expression, both tissues mutations, which were validated independently by real-time reverse...
Squamous cell carcinoma of the head and neck (HNSCC) is sixth most common type cancer in United States. The goal this study was to evaluate contribution estrogens development HNSCCs. Various lines derived from early- late-stage lesions were used characterize expression estrogen synthesis metabolism genes, including cytochrome P450 (CYP) 1B1, examine effect on gene expression, role CYP1B1 and/or motility, proliferation, apoptosis. Estrogen genes (CYP1B1, CYP1A1, catechol-o-methyltransferase,...