A5069206173- Spine and Intervertebral Disc Pathology
- Spinal Fractures and Fixation Techniques
- Musculoskeletal pain and rehabilitation
- Medical Imaging and Analysis
- Anesthesia and Pain Management
- DNA Repair Mechanisms
- Cervical and Thoracic Myelopathy
- DNA and Nucleic Acid Chemistry
- CRISPR and Genetic Engineering
- Spinal Hematomas and Complications
- Genetic factors in colorectal cancer
- Advanced Proteomics Techniques and Applications
- Pelvic and Acetabular Injuries
- Plant Genetic and Mutation Studies
- Genomics and Chromatin Dynamics
- Molecular Biology Techniques and Applications
- Spinal Dysraphism and Malformations
- Scoliosis diagnosis and treatment
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- Advanced biosensing and bioanalysis techniques
- Shoulder Injury and Treatment
- Mass Spectrometry Techniques and Applications
- Chromosomal and Genetic Variations
University of British Columbia
2011-2025
Creighton University
2010-2025
Icahn School of Medicine at Mount Sinai
2025
Valley Health System
2025
Desert Institute For Spine Care
2015-2024
Imperial College London
2024
University of New Mexico
2016-2023
Liverpool Hospital
2023
Southern Methodist University
2022
Centre for Addiction and Mental Health
2022
A retrospective review involving 307 consecutive cases of lumbar disc herniation managed by posterolateral endoscopic discectomy was conducted.To describe a contemporary decompression technique for radiculopathy secondary to herniation; evaluate the efficacy as it is applied including primary herniation, reherniation, intracanal and extracanal report outcome complications.The concept percutaneous nucleotomy introduced in 1973. The development related equipment had witnessed slow lengthy...
With the completion of genome sequencing projects, emphasis in genomics has shifted from analyzing sequences to understanding gene function, and effective reverse genetic strategies are increasingly demand. Here we report adaptations targeting induced local lesions genomes (TILLING)
Objectives Social media platforms are increasingly being used to disseminate mental health information online. User-generated content about attention-deficit/hyperactivity disorder (ADHD) is one of the most popular topics on video-sharing social platform TikTok. We sought investigate quality TikTok videos ADHD. Method The top 100 ADHD uploaded by video creators were classified as misleading, useful, or personal experience. Descriptive and quantitative characteristics obtained. Patient...
We have discovered a useful new reagent for mutation detection, novel nuclease CEL I from celery. It is specific DNA distortions and mismatches pH 6 to 9. Incision on the 3′-side of mismatch site in one two strands heteroduplex. I-like nucleases are found many plants. report here that simple method enzyme detection using can efficiently identify mutations polymorphisms. To illustrate efficacy this approach, exons BRCA1 gene were amplified by PCR primers 5′-labeled with fluorescent dyes...
The DNA mismatch repair (MMR) is a specialized system, highly conserved throughout evolution, involved in the maintenance of genomic integrity. To identify novel human genes that may function MMR, we employed yeast interaction trap. Using MMR protein MLH1 as bait, cloned MED1. MED1 forms complex with MLH1, binds to methyl-CpG-containing DNA, has homology bacterial glycosylases/lyases, and displays endonuclease activity. Transfection mutant lacking methyl-CpG-binding domain (MBD) associated...
The cloned uvrA and uvrB genes of Escherichia coli K-12 were amplified by linkage to the PL promoter plasmid pKC30. uvrC gene was in high-copy-number pRLM 24. three products (purified each case greater than 95% purity) ATP are required effectively incise UV-damaged DNAs. uvrABC proteins bind tightly damaged sites DNA, requiring initial attachment protein presence before productive binding proteins. Using a tandem double insert lac p-o region as DNA substrate for complex analyzing incision...
Abstract Background Language impairment is an important marker of neurodegenerative disorders. Despite this, there no universal system terminology used to describe these impairments and large inter-rater variability can exist between clinicians assessing language. The use natural language processing (NLP) automated speech analysis (ASA) emerging as a novel potentially more objective method assess in individuals with mild cognitive (MCI) Alzheimer’s dementia (AD). No studies have analyzed how...
The androgen receptor (AR) is one of the most studied drug targets for treatment prostate cancer. However, all current anti-androgens directly interact with AR at binding site, which prone to resistant mutations, calling new strategies inhibition. study represents first attempt use virtual screening identify inhibitors activation function-2 (AF2) human AR. By combining large-scale docking experimental approaches, we were able several small molecules that AF2 and effectively prevent...
The most common causes of failed back surgery are residual or recurrent herniation, foraminal fibrosis and stenosis that is ignored, untreated, undertreated. Residual ache may also be from facetal denervation scarring the paraspinal muscles.(1-6) original surgeon advise his patient nothing more can done on basis opinion nerve was visually decompressed by surgery, supported improved post-op imaging follow-up studies such as EMG conduction velocity studies. Post-op electrophysiological...
To analyze long-term clinical outcomes of endoscopic transforaminal foraminoplasty for foraminal stenosis.Long-term 5-year MacNab outcomes, visual analogue scale (VAS) scores, complications, and unintended aftercare were analyzed in a series 86 patients who underwent stenosis.At minimum follow-up, excellent results according to the criteria obtained 32 (37.2%), Good 40 (46.5%), fair 11 (12.8%), poor 3 (3.5%), respectively. The mean preoperative VAS was 6.15. postoperative last follow-up...
Personalized care models are dominating modern medicine. These rooted in teaching future physicians the skill set to keep up with innovation. In orthopedic surgery and neurosurgery, education is increasingly influenced by augmented reality, simulation, navigation, robotics, some cases, artificial intelligence. The postpandemic learning environment has also changed, emphasizing online skill- competency-based incorporating clinical bench-top research. Attempts improve work–life balance...
CEL I, isolated from celery, is the first eukaryotic nuclease known that cleaves DNA with high specificity at sites of base-substitution mismatch and distortion. The enzyme requires Mg2+ Zn2+ for activity, a pH optimum neutral pH. We have purified I 33 000-fold to apparent homogeneity. A key improvement use α-methyl-mannoside in purification buffers overcome aggregation glycoproteins endogenous lectins. SDS gel electrophoresis band homogeneous without removal its carbohydrate moieties, was...
Objectives: There are currently no diagnostic indicators that consistently reliable, obtainable, and conclusive for diagnosing risk-stratifying pancreatic cysts. Proteomic analyses were performed to explore cyst fluids yield effective biomarkers. Methods: We have prospectively recruited 20 research participants prepared their specifically proteomic analyses. approaches applied as follows: (1) matrix-assisted laser-desorption-ionization time-of-flight mass spectrometry peptidomics with...
Objectives. Test a miniaturized neurostimulator transforaminally placed at the dorsal root ganglion (DRG) and evaluate device's safety efficacy in treating failed back surgery syndrome (FBSS) low pain. Design. Pilot, two-phase study. Subjects. Eleven subjects with chronic intractable neuropathic trunk and/or lower limbs pain were included. Methods. The system consisted of an implantable, stimulator, provided by Stimwave Technologies (Freedom-4) external transmitter. Only one stimulator per...