A5048433029- Antimicrobial Peptides and Activities
- Lipid Membrane Structure and Behavior
- RNA and protein synthesis mechanisms
- Biochemical and Structural Characterization
- Photodynamic Therapy Research Studies
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Nanoplatforms for cancer theranostics
- Chemical Synthesis and Analysis
- Calpain Protease Function and Regulation
- Diamond and Carbon-based Materials Research
- Connexins and lens biology
- Protein Structure and Dynamics
- Machine Learning in Bioinformatics
- Phenothiazines and Benzothiazines Synthesis and Activities
- Protein Hydrolysis and Bioactive Peptides
- Invertebrate Immune Response Mechanisms
- Glycosylation and Glycoproteins Research
- RNA Interference and Gene Delivery
- Advanced Drug Delivery Systems
- Nanoparticle-Based Drug Delivery
- Genomics and Phylogenetic Studies
- Inhalation and Respiratory Drug Delivery
- Polydiacetylene-based materials and applications
London South Bank University
2014-2023
National Institute of Academic Anaesthesia
2019
Roswell Park Comprehensive Cancer Center
2017-2019
University of Central Lancashire
2007-2018
Institute of Nanotechnology
2012-2014
Dean College
2004
Clemson University
1997
Utrecht University
1993
University of Liverpool
1990
The photodynamic antibacterial properties of a closely related series phenothiazinium dyes were tested against several pathogenic strains Staphylococcus aureus, four which methicillin-resistant. Illumination the photosensitisers at fluence rate 1.75 mW cm-2 generally resulted in enhancement activity liquid culture and greater efficacy than methicillin analogue flucloxacillin. For methylene blue, dimethyl blue new illumination led to increases bactericidal < or = 16-fold, typically 4-fold. In...
Aurein 2.6-COOH and aurein 3.1-COOH were studied along with their naturally occurring C-terminally amidated analogues. Circular dichroism (CD) molecular dynamic (MD) simulations used to study the effects of amidation on interaction antimicrobial peptides (AMPs) lipid bilayers. CD measurements MD analysis suggested that both peptide analogues predominantly random coil adopted low levels α-helical structure in solution (<30%) presence a bilayer formed stable structure. In general, have greater...
The photodynamic antibacterial properties of a closely related series commercially available phenothiazinium dyes were tested against range pathogenic strains Gram-positive (Staphylococcus aureus, Enterococcus faecalis, Bacillus cereus) and Gram-negative organisms (Escherichia coli, Pseudomonas aeruginosa). photosensitisers illuminated using non-laser light source at fluence 1.75 mW cm-2 this resulted in the enhancement activity liquid culture. In several cases, illumination considerable...
Understanding which peptides and proteins have the potential to undergo amyloid formation what driving forces are responsible for amyloid-like fiber stabilization remains limited. This is mainly because that can structural changes, lead formation, quite diverse share no obvious sequence or homology, despite similarity found in fibrils. To address these issues, a novel approach based on recursive feature selection feed-forward neural networks was undertaken identify key features highly...
The graphene oxide (GO) sheet has been considered one of the most promising carbon derivatives in field material science for past few years and shown excellent tumor-targeting ability, biocompatibility low toxicity. We have endeavored to conjugate paclitaxel (PTX) GO molecule investigate its anticancer efficacy.We conjugated drug PTX aminated PEG chains on sheets through covalent bonds get GO-PEG-PTX complexes. tissue distribution efficacy were then investigated using a B16 melanoma...
To gain insight into the effects of amidation on mechanism membrane interaction, we studied two peptides modelin-5-COOH and modelin-5-CONH(2) found they exhibit high surface activities (23.2 27.1 mN/m, respectively). When were tested against Escherichia coli, was seen to increase efficacy approximately 10-fold. Our results demonstrated that both adopted low levels α-helix in solution (<20%); however, presence E. coli lipid extract, had a greater propensity (69%) than (32%) generate α-helical...
The toxicities and phototoxicities of methylene blue its two methylated derivatives were measured against one standard three vancomycin-resistant pathogenic strains Enterococcus spp. Each the compounds was bactericidal exhibited photobactericidal activity on illumination at a 'light' dose 6.3 J/cm(2) or more strains. Increased in is thought to be due their higher hydrophobicities allowing greater interaction with bacterial cell wall. In addition, inherent photosensitizing efficacies.
Phenothiazinium dyes, and derivatives, were tested for toxicity to Escherichia coli Staphylococcus aureus. The dyes generally lipophilic (log P>1) showed inherent dark (minimum lethal concentrations: 3.1-1000 microM). Dye illumination (total light dose of 3.15 J cm(-1) over 30 min) led up eight-fold reductions in minimum concentrations. Most the illuminated significant relative singlet oxygen yields (phi'delta: 0.18-1.35) suggesting a type II mechanism generating phototoxic response....