A5081025221- Monoclonal and Polyclonal Antibodies Research
- Cancer-related gene regulation
- Immunotherapy and Immune Responses
- Head and Neck Cancer Studies
- Advanced biosensing and bioanalysis techniques
- Cancer Immunotherapy and Biomarkers
- S100 Proteins and Annexins
- Estrogen and related hormone effects
- Advanced Biosensing Techniques and Applications
- RNA modifications and cancer
- Cancer-related Molecular Pathways
- Cell Adhesion Molecules Research
- Cancer Cells and Metastasis
- Wnt/β-catenin signaling in development and cancer
- Lung Cancer Treatments and Mutations
- Cancer-related molecular mechanisms research
- Protease and Inhibitor Mechanisms
- Radiopharmaceutical Chemistry and Applications
- Immune Cell Function and Interaction
- Voice and Speech Disorders
- Ion channel regulation and function
- Hippo pathway signaling and YAP/TAZ
- Nanoparticle-Based Drug Delivery
- HER2/EGFR in Cancer Research
- RNA Interference and Gene Delivery
Instituto de Investigación Sanitaria del Principado de Asturias
2015-2024
Universidad de Oviedo
2015-2024
Centro de Investigación Biomédica en Red de Cáncer
2017-2024
Instituto de Salud Carlos III
2018-2024
Central University Hospital of Asturias
2011-2023
Universidad de Salamanca
2020
Centre for Biomedical Network Research on Rare Diseases
2020
Imperial College London
2006
Abstract We investigated the status of PI 3‐kinase/AKT/PTEN signaling pathway in a series 117 head and neck squamous cell carcinomas (HNSCC) search for molecular alterations genes/proteins with potential prognostic value. For this purpose, PIK3CA AKT2 gene amplification was assessed by multiplex Quantitative Real‐Time PCR. Protein expression AKT, p‐AKT, p110α PTEN determined Western blot. allelic loss evaluated microsatellite analysis. ‐exon 5 screened point mutations PCR‐SSCP. Homozygous...
Abstract The TINCR ( T erminal differentiation- I nduced N on- C oding R NA) gene is selectively expressed in epithelium tissues and involved the control of human epidermal differentiation wound healing. Despite its initial report as a long non-coding RNA, locus codes for highly conserved ubiquitin-like microprotein associated with keratinocyte differentiation. Here we identification tumor suppressor squamous cell carcinoma (SCC). upregulated by UV-induced DNA damage TP53-dependent manner...
Abstract Background Tumor mutational burden (TMB) has been demonstrated to predict the response immune checkpoint inhibitors (ICIs) in various cancers. However, role of TMB head and neck squamous cell carcinoma (HNSCC) not yet specifically addressed. Since HNSCC patients exhibit a rather limited ICIs, there is an unmet need develop predictive biomarkers improve patient selection criteria clinical benefit ICI treatment. Methods We conducted systematic review meta-analysis according Preferred...
Melatonin is an indole hormone produced mainly by the pineal gland. We have previously demonstrated that melatonin interferes with estrogen (E2) signaling in MCF7 cells impairing receptor (ER) pathways. Here we present characterization of its mechanism action showing a specific inhibitor E2-induced ERα-mediated transcription both response element- and AP1-containing promoters, whereas ERβ-mediated transactivation not inhibited or even activated at certain promoters. show sensitivity MCF-7 to...
Hey1 is a member of the basic helix-loop-helix-Orange family transcriptional repressors that mediate Notch signaling. Here we show transcription from androgen-dependent target genes inhibited by and expression constitutively active form capable repressing transactivation endogenous androgen receptor (AR). Our results indicate functions as corepressor for AF1 in AR, providing mechanism cross talk between androgen-signaling pathways. colocalizes with AR epithelia patients benign prostatic...
Recent studies support an important role for human papillomavirus (HPV) in oropharyngeal squamous cell carcinomas (OPSCC), although the incidence varies widely depending on geographic location and time period studied. The aim of this study was to determine proportion HPV a large cohort OPSCC northern Spain years 1990-2009. Clinical records paraffin embedded tumor specimens 248 consecutive patients surgically treated (140 tonsillar 108 base tongue) between 1990 2009 were retrieved. cases...
The PI3K/AKT/mTOR signaling pathway has emerged as one of the most frequently deregulated in head and neck squamous cell carcinomas (HNSCC). Numerous alterations various upstream downstream components have been described; however, their prognostic significance impact on HNSCC patient survival remains to be established. This was addressed using an unbiased cohort 93 consecutive homogeneous surgically treated patients results confirmed 432 patients. Our findings reveal high prevalence S6...
The immune checkpoint PD-1 and its ligand PD-L1 are involved in the induction of immunological tolerance solid tumors including oral squamous cell carcinoma (OSCC). aim study was to establish clinical prognostic significance OSCC.Tissue microarrays 125 resected OSCC were stained with two different commercially available antibodies (clones E1L3N 22C3), alongside immunostaining.PD-L1 expression more than 10% tumor cells associated poorer survival, established as a clinically relevant cut-off...
Rationale: The characterization of new genetic alterations is essential to assign effective personalized therapies in non-small cell lung cancer (NSCLC). Furthermore, finding stratification biomarkers for successful therapies. Molecular YES1, a member the SRC (proto-oncogene tyrosine-protein kinase Src) family kinases (SFKs), can be found significant subset patients with cancer.Objectives: To evaluate YES1 (v-YES-1 Yamaguchi sarcoma viral oncogene homolog 1) alteration as therapeutic target...
The use of immune-checkpoint inhibitors has drastically improved the management patients with non-small cell lung cancer (NSCLC), but innate and acquired resistances are hurdles needed to be solved. Immunomodulatory drugs that can reinvigorate immune cytotoxic activity, in combination antiprogrammed death 1 (PD-1) antibody, a great promise overcome resistance. We evaluated impact SRC family kinases (SFKs) on NSCLC prognosis, immunomodulatory effect SFK inhibitor dasatinib, anti-PD-1,...
The present study shows that melatonin prevents, within the first cell cycle, estradiol-induced growth of synchronized MCF7 breast cancer cells. By using nuclear extracts these cells, we examined binding estradiol-estrogen receptor complexes to estrogen-responsive elements and found addition estradiol whole cells activates estrogen DNA whereas blocks this interaction. contrast, neither affects its nor localization. Moreover, also show stimulates DNA, but activation is prevented by melatonin....
Estrogen receptors (ERs) play critical roles in both normal mammary gland development and the formation progression of breast tumors, constituting a major therapeutic target for cancer treatment. We have previously described that ER transcriptional activity is potentiated by BAF57, core subunit mammalian SWI/SNF chromatin remodeling complex. Here we provide evidence demonstrating an important role BAF57 as regulator functions cells. Different experimental manipulations leading to abrogation...
This study investigates for the first time crosstalk between stromal fibroblasts and cancer stem cell (CSC) biology in head neck squamous carcinomas (HNSCC), with ultimate goal of identifying effective therapeutic targets. The effects conditioned media from cancer-associated (CAFs) normal (NFs) on CSC phenotype were assessed by combining functional expression analyses HNSCC-derived lines. Further characterization CAFs NFs secretomes mass spectrometry was followed pharmacologic target...
Melatonin mitigates cancer initiation, progression and metastasis through inhibition of both the synthesis estrogens transcriptional activity estradiol-ER (Estrogen receptor) complex in estrogen-dependent breast cell line MCF-7. Moreover, melatonin improves sensitivity MCF-7 to chemotherapeutic agents protects against their side effects. It has been described that potentiates anti-proliferative effects doxorubicin; however, molecular changes involving gene expression activation/inhibition...
Tumor-associated macrophages (TAMs) can be polarized into antitumoral M1 and protumoral immunosuppressive M2 macrophages. This study investigated the clinical relevance of TAM infiltration in oral squamous cell carcinoma (OSCC), evaluating CD68 (M1 macrophage marker) CD163 expression (M2 tumor nests surrounding stroma. Immunohistochemical analysis both stromal/tumoral CD68+ CD163+ TAMs was performed paraffin-embedded tissue specimens from 125 OSCC patients, correlated with data. Potential...
Purpose The aim of this study was to investigate the prognostic significance preoperative inflammatory markers in peripheral blood patients with oral squamous cell carcinoma (OSCC), and establish correlations infiltrate macrophages lymphocytes local immune tumor microenvironment (TME). Materials Methods Neutrophil-lymphocyte ratio (NLR), platelet-lymphocyte (PLR), lymphocyte-monocyte (LMR), systemic immune-inflammation index (SII) were retrospectively evaluated a cohort 348 OSCC patients,...
Introduction: Upregulated microtubule-associated serine/threonine kinase like (MASTL), a cell cycle required for progression through mitosis, expression has been associated to poor prognosis. This study is aimed investigate the clinical relevance of MASTL in oral squamous carcinoma (OSCC), and possible mechanistic link with epithelial-mesenchymal transition (EMT). Methods: Immunohistochemical analysis MASTL, E-cadherin, Vimentin, Smad7 was performed paraffin-embedded tissue specimens from...
Head and neck squamous cell carcinoma (HNSCC) is a highly aggressive cancer, with limited therapeutic options high mortality rate, primarily due to metastasis recurrence. Tumor-stroma interactions, namely cancer-associated fibroblasts (CAFs), are pivotal in shaping HNSCC progression. CAFs remodel the extracellular matrix (ECM) secrete factors vesicles that promote tumor growth metastasis. The interplay between autophagy endosomal/exosomal pathways has been suggested regulate cellular...