A5100669611- Sarcoma Diagnosis and Treatment
- Cancer-related Molecular Pathways
- Cancer Cells and Metastasis
- Cancer-related gene regulation
- CAR-T cell therapy research
- RNA modifications and cancer
- Beetle Biology and Toxicology Studies
- Head and Neck Cancer Studies
- Ethics and bioethics in healthcare
- Estrogen and related hormone effects
- Philosophical Thought and Analysis
- Epigenetics and DNA Methylation
- Genetic factors in colorectal cancer
- S100 Proteins and Annexins
- Cancer Diagnosis and Treatment
- Protease and Inhibitor Mechanisms
- Lung Cancer Treatments and Mutations
- Wnt/β-catenin signaling in development and cancer
- Diverse academic and cultural studies
- Cancer Genomics and Diagnostics
- Philosophical and Cultural Analysis
- RNA Interference and Gene Delivery
- Corneal Surgery and Treatments
- Educational and Social Studies
- PI3K/AKT/mTOR signaling in cancer
Hospital Universitario de San Vicente Fundación
2024
Instituto de Investigación Sanitaria del Principado de Asturias
2007-2023
Universidad de Oviedo
2001-2023
Centro de Investigación Biomédica en Red de Cáncer
2017-2023
Ministry of Health
2023
Government of Catalonia
2023
Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública
2023
Instituto de Salud Carlos III
2023
Pontificia Universidad Católica del Ecuador
2020
Institut Català de Traumatologia i Medicina de l'Esport
2020
Abstract We investigated the status of PI 3‐kinase/AKT/PTEN signaling pathway in a series 117 head and neck squamous cell carcinomas (HNSCC) search for molecular alterations genes/proteins with potential prognostic value. For this purpose, PIK3CA AKT2 gene amplification was assessed by multiplex Quantitative Real‐Time PCR. Protein expression AKT, p‐AKT, p110α PTEN determined Western blot. allelic loss evaluated microsatellite analysis. ‐exon 5 screened point mutations PCR‐SSCP. Homozygous...
Glaucoma is a progressive ocular syndrome characterized by degeneration of the optic nerve and irreversible visual field loss. Elevated intraocular pressure (IOP) main risk factor for glaucoma. Increased IOP result an imbalance between synthesis outflow aqueous humor (AH). Blocking β2 adrenergic receptor (ADRB2) has shown to reduce decreasing production AH at ciliary body (CB). SYL040012 siRNA designed specifically silence ADRB2 currently under development glaucoma treatment. Here, we show...
Purpose: To evaluate the efficacy and safety of SYL1001, a short interfering (si) RNA targeting transient receptor potential cation channel subfamily V member 1 (TRPV1), for treatment dry eye disease (DED). Methods: This study combines phase I two II clinical trials to test different doses SYL1001 in total 156 healthy subjects patients with DED. After 10 days treatment, primary endpoints were effect on (1) scoring Visual Analogue Scale (VAS) Ocular Surface Disease Index (OSDI)...
The objective of this study was to evaluate ocular tolerance, safety, and effect on intraocular pressure (IOP) a topically administered small interfering RNA; SYL040012, healthy volunteers. an open-label, controlled, single-center comprised two intervals that enrolled 30 subjects having IOP below 21 mmHg. SYL040012 one eye as single dose six during interval 1. During 2 different doses were daily basis separate groups 12 each, over period 7 days. contralateral evaluated but not served control...
The PI3K/AKT/mTOR signaling pathway has emerged as one of the most frequently deregulated in head and neck squamous cell carcinomas (HNSCC). Numerous alterations various upstream downstream components have been described; however, their prognostic significance impact on HNSCC patient survival remains to be established. This was addressed using an unbiased cohort 93 consecutive homogeneous surgically treated patients results confirmed 432 patients. Our findings reveal high prevalence S6...
The immunosuppressive and antiinflammatory actions of glucocorticoid hormones are mediated by their transrepression activating protein-1 (AP-1) nuclear factor-kappa B (NFκB) transcription factors. Inhibition the c-Jun NH2-terminal kinase (JNK) signaling pathway, main mediator AP-1 activation, has been described in extracts hormone-treated cells. Here, we show confocal laser microscopy, enzymatic assays, immunoblotting that synthetic dexamethasone inhibited tumor necrosis factor α...
To study the loss of heterozygosity and presence mutations at p53, p16/CDKN2, APC genes in Barrett's oesophagus, low grade dysplastic oesophageal epithelium, adenocarcinoma oesophagus; to relate alterations these with progression from oesophagus adenocarcinoma.DNA was extracted paraffin blocks containing tissue (12 samples), dysplasia (15 cases), (14 cases). Loss (LOH) p16, determined by comparing autoradiographic patterns several microsatellite markers between normal malignant counterpart....
AIMS: To analyse the allele frequencies of DNA polymorphisms at genes for cytochromes P450IIE1 and P450IID6, N-acetyltransferase-2, glutathione S-transferase-M1 in patients with head neck squamous cell carcinoma, an attempt to define genetic factors involved susceptibility this cancer, which is strongly associated tobacco consumption. METHODS: Determination restriction fragment length polymorphism (RFLP) P450IIE1/P450IID6 NAT2 genes, presence homozygous deletion GSTM1 gene, 200 controls 75...
To determine if whole body vibration training (WBVT) improves muscular force and modifies functional capacity parameters in patients with severe chronic obstructive pulmonary disease (COPD).We performed a randomized controlled trial the outpatient Physical Medicine Rehabilitation Department of general hospital. Sixty stable male COPD mean forced expiratory volume 1 s (FEV1) 34.3% (predicted) were enrolled 51 completing study. Participants into two groups: Whole Body Vibration Training Group...
Deregulated SRC/FAK signaling leads to enhanced migration and invasion in many types of tumors. In myxoid round cell liposarcoma (MRCLS), an adipocytic tumor characterized by the expression fusion oncogene FUS-CHOP, SRC have been found as one most activated kinases. Here we used a cell-of-origin model MRCLS line thoroughly characterize mechanisms induced FUS-CHOP using vitro (3D spheroid assays) vivo (chicken chorioallantoic membrane model) approaches. SRC-FAK increased invasive ability...
To elucidate the role of epithelial-mesenchymal transition markers in gastroenteropancreatic neuroendocrine tumors (GEP NETs) and potential usefulness their clinical management. One hundred ten GEP NET paraffin-embedded samples were immunohistochemically analyzed for E-cadherin, N-cadherin, β-catenin, vimentin, Snail1, Snail2, Twist, Foxc2 protein expression. The 5-year survival rate was reduced those patients showing high Snail1 levels, a cytoplasmic E-cadherin pattern, N-cadherin...
Pulmonary neuroendocrine tumors (Pulmonary NETs) include a wide spectrum of tumors, from the low-grade typical carcinoid (TC) and intermediate-grade atypical (AC), to high-grade large-cell carcinoma (LCNEC) small-cell (SCLC). Epithelial Mesenchymal Transition (EMT) is process initially recognised during several critical stages embryonic development, which has more recently been implicated in promoting invasion metastasis. The initial stage EMT begins with deregulation adhesion molecules,...
Stemness in sarcomas is coordinated by the expression of pluripotency factors, like SOX2, cancer stem cells (CSC). The role SOX2 tumor initiation and progression has been well characterized osteosarcoma. However, pro-tumorigenic features have scarcely investigated other sarcoma subtypes. Here, we show that depletion dramatically reduced ability undifferentiated pleomorphic (UPS) to form tumorspheres initiate growth. Conversely, overexpression resulted increased vivo tumorigenicity. Moreover,...
To describe the characteristics of infections occurring in immediate postoperative period orthotopic liver transplantation (OLT) children a pediatric intensive care unit (PICU) and associated risk factors.Retrospective cohort study.Multidisciplinary 16-bed tertiary university hospital.One hundred thirty-three patients (range 6M to 22 yr) who underwent 176 transplantations were admitted for at least 48 h.A total 180 infectious episodes (IE) occurred 78 (59%) 96 (54.5%) (1.35 IE/patient; 1.02...
<h3>Objective</h3> Describe the extent to which tobacco industry marketing tactics incorporated American Indian culture and traditional tobacco. <h3>Methods</h3> A keyword search of documents was conducted using document archives from Truth Tobacco Documents Library. (n=76) were analysed for themes. <h3>Results</h3> have since at least 1930s, with these prominently highlighted during 1990s Natural Spirit cigarettes. revealed use imagery such as headdresses other cultural symbols in product...
Glucocorticoid hormones, retinoids, and vitamin D3 display anti‐angiogenic activity in tumor‐bearing animals. However, despite their vivo effect on the tumor vasculature little is known about mechanism of action. Here we show that synthetic glucocorticoid dexamethasone (Dex) retinoic acid (RA) inhibit activation c‐Jun N‐terminal kinase (JNK) extracellular‐regulated (ERK) signalling pathways by pro‐angiogenic agents necrosis factor vascular endothelial growth cells. In contrast, Dex RA failed...
Abstract Objective Tumors arising from different sites of the head and neck area have clinical behavior. However, most studies on genetic alterations in squamous cell carcinomas do not make a distinction between within this area. The objective study is to compare three (larynx, oropharynx, hypopharynx). Study Design Prospective study. Methods Thirty‐eight laryngeal, 29 oropharyngeal, 37 hypopharyngeal were studied. DNA tumor healthy tissue was evaluated for amplification oncogenes at 11q13...
Abstract Sarcomas are mesenchymal cancers with poor prognosis, representing about 20% of all solid malignancies in children, adolescents, and young adults. Radio- chemoresistance common features sarcomas warranting the search for novel prognostic predictive markers. GARP/LRRC32 is a TGF-β-activating protein that promotes immune escape dissemination various cancers. However, if GARP affects tumorigenicity treatment resistance not known. We show expressed by human osteo-, chondro-,...
Sarcomas comprise a group of aggressive malignancies with very little treatment options beyond standard chemotherapy. Reposition approved drugs represents an attractive approach to identify effective therapeutic compounds. One example is mithramycin (MTM), natural antibiotic which has demonstrated strong antitumour activity in several tumour types, including sarcomas. However, its widespread use the clinic was limited by poor toxicity profile.In order improve index MTM, we have loaded MTM...
AIMS: To study the loss of heterozygosity at short arm chromosome 3 in primary tumours from patients with squamous cell carcinoma head and neck; to determine whether FHIT gene, mapped 3p14.2 CTNNB1 (beta-cat) 3p21, are deleted or mutated these tumours. METHODS: DNA was extracted fresh Loss assessed by microsatellite analysis following markers: D3S1283 D3S1286 (3p24), D3S966 (3p21), D3S1300 (3P14.2). Homozygous deletion determined radioactive multiplex polymerase chain reaction exons 5 6...
CD44 splice variants are assumed to have a critical role in the malignant progression of many human tumors. However, clinical significance expression is not yet understood. The aim this study was investigate prognostic CD44s and CD44v6 isoforms squamous cell carcinomas supraglottic larynx. determined by immunohistochemical analysis paraffin-embedded tissue specimens from 101 patients. There significant correlation between decreased or poorer histologic differentiation. No relationship...