A5026437668- Adipose Tissue and Metabolism
- Mitochondrial Function and Pathology
- Liver physiology and pathology
- Liver Disease Diagnosis and Treatment
- Metabolomics and Mass Spectrometry Studies
- Pancreatic function and diabetes
- Diet and metabolism studies
- Metabolism and Genetic Disorders
- Lipid metabolism and biosynthesis
- Cancer, Hypoxia, and Metabolism
- Autophagy in Disease and Therapy
- Epigenetics and DNA Methylation
- Sirtuins and Resveratrol in Medicine
- Metabolism, Diabetes, and Cancer
- Amino Acid Enzymes and Metabolism
- Muscle metabolism and nutrition
- Neuroendocrine Tumor Research Advances
- Pancreatitis Pathology and Treatment
- Adipokines, Inflammation, and Metabolic Diseases
- Cardiovascular Disease and Adiposity
- Reproductive Physiology in Livestock
- Lipid metabolism and disorders
- Genomics and Chromatin Dynamics
- Soft tissue tumor case studies
- Intraperitoneal and Appendiceal Malignancies
University of Lausanne
2019-2025
University Hospital Heidelberg
2024
Heidelberg University
2024
Medical University of Graz
2023-2024
Cancer Research Institute
2018-2021
University of Pennsylvania
2018-2021
UPMC Hillman Cancer Center
2018-2021
Graz University of Technology
2016-2021
Centre National de la Recherche Scientifique
2018
Délégation Paris 5
2018
Abstract Histone acetylation depends on the abundance of nucleo-cytoplasmic acetyl-CoA. Here, we present a novel route for cytoplasmic acetyl-CoA production in brown adipocytes. N-acetylaspartate (NAA) is highly abundant brain metabolite catabolized by aspartoacylase yielding aspartate and acetate. The latter can be further used production. Prior to this work, presence NAA has not been described show that accumulation decreases adipocyte phenotype. We increased intracellular concentrations...
The discovery of significant amounts metabolically active brown adipose tissue (BAT) in adult humans renders it a promising target for anti-obesity therapies by inducing weight loss through increased energy expenditure. components the N-acetylaspartate (NAA) pathway are highly abundant BAT. Aspartate N-acetyltransferase (Asp-NAT, encoded Nat8l) synthesizes NAA from acetyl-CoA and aspartate increases expenditure adipocytes. However, exact mechanism how contributes to accelerated mobilization...
Metabolic reprogramming is considered as a hallmark of cancer and clinically exploited novel target for therapy. The E2F transcription factor-1 (E2F1) regulates various cellular processes, including proliferative metabolic pathways, acts, depending on the molecular context, an oncogene or tumor suppressor. latter evident by observation that E2f1-knockout mice develop spontaneous tumors, uterine sarcomas. This dual role warrants detailed investigation how E2F1 loss impacts pathways related to...
Both cross-presentation of antigens by dendritic cells, a key pathway triggering T cell immunity and immune tolerance, survival several pathogens residing in intracellular vacuoles are intimately linked to delayed maturation vesicles containing internalized microbes. However, how early endosome or phagosome identity is maintained incompletely understood. We show that Toll-like receptor 4 (TLR4) Fc ligation induces interaction the GTPase Rab14 with kinesin KIF16b mediating plus-end-directed...
Lysosomal acid lipase (LAL) is the only known enzyme, which hydrolyzes cholesteryl esters and triacylglycerols in lysosomes of multiple cells tissues. Here, we explored role LAL brown adipose tissue (BAT). LAL-deficient (Lal-/-) mice exhibit markedly reduced UCP1 expression BAT, modified BAT morphology with accumulation lysosomes, mitochondrial dysfunction, consequently leading to regular hypothermic events kept at room temperature. Cold exposure resulted lipid uptake into thereby...
Chromatin is the template for replication and transcription in eukaryotic nucleus, which needs to be defined composition structure before these processes can fully understood.We report an isolation protocol targeted purification of specific genomic regions their native chromatin context from Saccharomyces cerevisiae.Subdomains multicopy ribosomal DNA locus containing units RNA polymerases I, II or III autonomous sequence were independently purified sufficient amounts purity analyze protein...
The dynamic regulation of metabolic pathways can be monitored by stable isotope tracing. Yet, many metabolites are part distinct processes within different subcellular compartments. Standard tracing experiments relying on analyses in whole cells may not accurately reflect compartmentalized processes. Analysis metabolism and the interplay between compartments potentially achieved followed fractionation. Although it is recognized that take place during biochemical fractionation cells, a clear...
Abstract Purpose To evaluate the diagnostic yield of contrast-enhanced ultrasound (CEUS)-guided biopsy retroperitoneal masses (RMs). Materials and Methods Between 2006 2023, 87 patients presented at our US center for an RM. In all biopsies, CEUS was performed prior to intervention. The technical success rate biopsy, presence tissue in solid tumor samples, accuracy occurrence post-interventional complications were evaluated. Results A US-guided could be conducted 84/87 cases (96.6%). 3/87...
Adipocyte plasma membrane-associated protein (APMAP) has been described as an adipogenic factor in 3T3-L1 cells with unknown biochemical function; we therefore aimed to investigate the physiologic function of APMAP vivo We generated Apmap-knockout mice and challenged them obesogenic diet their metabolic phenotype. identified a novel truncated adipocyte-specific isoform that is produced by alternative transcription. Mice lacking full-length protein, only expressed humans, have improved...
ObjectivesTo evaluate the value of contrast-enhanced ultrasound (CEUS) perfusion patterns in differentiation benign and malignant retroperitoneal masses (RMs).MethodsBetween 2006 2023, 122 consecutive patients with an RM visualizable by B-mode US were investigated additionally CEUS. On CEUS, extent enhancement (classified as marked, reduced, or absent) homogeneity (HE; classified homogeneous inhomogeneous) evaluated. Subsequently, malignancy rate according to CEUS was determined.ResultsOn...
Article12 July 2020Open Access Source DataTransparent process Hypothalamic CDK4 regulates thermogenesis by modulating sympathetic innervation of adipose tissues Judit Castillo-Armengol orcid.org/0000-0001-7833-0569 Center for Integrative Genomics, University Lausanne, Switzerland Search more papers this author Valentin Barquissau Sarah Geller Honglei Ji Ilenia Severi Department Experimental and Clinical Medicine, Marche Polytechnic University, Ancona, Italy Wiebe Venema Eric Aria Fenandez...
Abstract Purpose Upper tract urothelial carcinoma (UTUC) represents an often aggressive malignancy associated with poor prognosis. Therefore, finding reliable prognostic biomarkers in patients undergoing curative surgery for improved risk stratification is crucial. We evaluated the value of Fibrinogen/C-reactive protein (FC)-score a cohort surgically treated UTUC patients. Methods 170 radiologically and histologically verified who underwent radical between 1990 2020, were included. The...
-acetylaspartate (NAA) is synthesized by aspartate
Summary Quantitative sub-cellular metabolomic measurements can yield crucial insights into the roles of metabolites in cellular processes, but are subject to multiple confounding factors. We developed S table I sotope L abeling E ssential nutrients cell C ulture – ub-cellular F ractionation (SILEC-SF), which uses isotope labeled internal standard controls that present throughout fractionation and processing quantify acyl-Coenzyme A thioesters compartments by liquid chromatography-mass...
During severe or chronic hepatic injury, biliary epithelial cells (BECs) undergo rapid activation into proliferating progenitors, a crucial step required to establish regenerative process known as ductular reaction (DR). While DR is hallmark of liver diseases, including advanced stages non-alcoholic fatty disease (NAFLD), the early events underlying BEC are largely unknown. Here, we demonstrate that BECs readily accumulate lipids during high-fat diet feeding in mice and upon acid treatment...