A5014026116- Research Data Management Practices
- Scientific Computing and Data Management
- Endoplasmic Reticulum Stress and Disease
- Data Quality and Management
- Adipose Tissue and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Artificial Intelligence in Healthcare and Education
- Diet and metabolism studies
- Liver Disease Diagnosis and Treatment
- Autophagy in Disease and Therapy
- FOXO transcription factor regulation
- Sirtuins and Resveratrol in Medicine
- Dietary Effects on Health
- Cancer-related gene regulation
- Mitochondrial Function and Pathology
- Big Data and Business Intelligence
- Lipid metabolism and biosynthesis
- Genetics, Bioinformatics, and Biomedical Research
- Semantic Web and Ontologies
- Cell Image Analysis Techniques
- Liver Disease and Transplantation
- Metabolism, Diabetes, and Cancer
- Pancreatic function and diabetes
- Cancer, Hypoxia, and Metabolism
- Explainable Artificial Intelligence (XAI)
Graz University of Technology
2020-2024
Know Center Research GmbH (Austria)
2021-2024
Research Center Pharmaceutical Engineering (Austria)
2023-2024
Medical University of Graz
2016-2022
BioTechMed-Graz
2019
Changes in intra- and extracellular potassium ion (K+) concentrations control many important cellular processes related biological functions. However, our current understanding of the spatiotemporal patterns physiological pathological K+ changes is severely limited by lack practicable detection methods. We developed K+-sensitive genetically encoded, Förster resonance energy transfer-(FRET) based probes, called GEPIIs, which enable quantitative real-time imaging dynamics. GEPIIs as purified...
Distinct subcellular pH levels, especially in lysosomes and endosomes, are essential for the degradation, modification, sorting, accumulation, secretion of macromolecules. Here, we engineered a novel genetically encoded probe by fusing pH-stable cyan fluorescent protein (FP) variant, mTurquoise2, to highly pH-sensitive enhanced yellow protein, EYFP. This approach yielded ratiometric biosensor—referred as pH-Lemon—optimized live imaging distinct conditions within acidic cellular compartments....
Summary Background Probiotics may correct intestinal dysbiosis and proinflammatory conditions in patients with liver cirrhosis. Aim To test the effects of a multispecies probiotic on innate immune function, bacterial translocation gut permeability. Methods In randomised, double blind, placebo‐controlled study, stable cirrhotic out‐patients either received daily dose powder containing eight different strains (Ecologic Barrier, Winclove, Amsterdam, The Netherlands) ( n = 44) or placebo 36) for...
Cancer cells voraciously consume nutrients to support their growth, exposing metabolic vulnerabilities that can be therapeutically exploited. Here, we show in hepatocellular carcinoma (HCC) cells, xenografts, and patient-derived organoids fasting improves sorafenib efficacy acts synergistically sensitize sorafenib-resistant HCC. Mechanistically, noncanonically as an inhibitor of mitochondrial respiration, causing resistant depend on glycolysis for survival. Fasting, through reduction glucose...
CyVerse, the largest publicly-funded open-source research cyberinfrastructure for life sciences, has played a crucial role in advancing data-driven since 2010s. As technology landscape evolved with emergence of cloud computing platforms, machine learning and artificial intelligence (AI) applications, CyVerse enabled access by providing interfaces, Software as Service (SaaS), cloud-native Infrastructure Code (IaC) to leverage new technologies. services enable researchers integrate...
Abstract Recent studies revealed that mitochondrial Ca 2+ channels, which control energy flow, cell signalling and death, are macromolecular complexes basically consist of the pore-forming uniporter (MCU) protein, essential MCU regulator (EMRE), uptake 1 (MICU1). MICU1 is a regulatory subunit shields mitochondria from overload. Before identification these core elements, novel uncoupling proteins 2 3 (UCP2/3) have been shown to be fundamental for uptake. Here we clarify molecular mechanism...
Alcohol binge drinking is a dangerous habit, associated with neurological problems and inflammation. The impact of single alcohol on innate immunity, gut barrier microbiome was studied. In this cohort study 15 healthy volunteers received 2 ml vodka 40% v/v ethanol/kg body weight. Neutrophil function studied by flow cytometry; markers permeability inflammation (lactulose/mannitol/sucrose test, zonulin, calprotectin, diamino-oxidase) were NMR spectroscopy enzyme-linked immunosorbent assay in...
Senescence is characterized by a gradual decline in cellular functions, including changes energy homeostasis and decreased proliferation activity. As power plants, contributors to signal transduction, sources of reactive oxygen species (ROS) executors programmed cell death, mitochondria are unique position affect aging-associated processes decline. Notably, metabolic activation tightly linked Ca2+ due the -dependency several enzymes Krebs cycle, however, overload with triggers death...
Abstract Wnt-induced β-catenin-mediated transcription is a driving force for stem cell self-renewal during adult tissue homeostasis. Enhanced Wnt receptor expression due to mutational inactivation of the ubiquitin ligases RNF43 / ZNRF3 recently emerged as leading cause cancer development. Consequently, targeting canonical receptors such LRP5/6 holds great promise treatment subsets. Here, we employ CIS display technology identify single-domain antibody fragments (VHH) that bind LRP6 P3E3P4E4...
The SHARC Interest Group of the Research Data Alliance was established to improve research crediting and rewarding mechanisms for scientists who wish organise their data (and material resources) community sharing. This requires that are findable accessible on Web, comply with shared standards making them interoperable reusable in alignment FAIR principles. It takes considerable time, energy, expertise motivation. is imperative facilitate processes encourage share data. To aim, supporting...
Rationale : Patients with end-stage renal disease are characterized by increased cardiovascular and all-cause mortality because of advanced remodeling the macrovascular microvascular beds. Objective The aim this study was to determine whether retinal function can predict in patients disease. Methods Results In multicenter prospective observational ISAR (Risk Stratification End-Stage Renal Disease), data on dynamic vessel analysis were available a subcohort 214 dialysis (mean age, 62.6±15.0;...
Lipotoxic cardiomyopathy in diabetic and obese patients typically encompasses increased cardiac fatty acid (FA) uptake eventually surpassing the mitochondrial oxidative capacity. Lowering FA utilization via inhibition of lipolysis represents a strategy to counteract development lipotoxic heart dysfunction. However, defective triacylglycerol (TAG) catabolism oxidation humans (and mice) carrying mutated ATGL alleles provokes dysfunction questioning therapeutic approach decrease lipolysis....
The discovery of significant amounts metabolically active brown adipose tissue (BAT) in adult humans renders it a promising target for anti-obesity therapies by inducing weight loss through increased energy expenditure. components the N-acetylaspartate (NAA) pathway are highly abundant BAT. Aspartate N-acetyltransferase (Asp-NAT, encoded Nat8l) synthesizes NAA from acetyl-CoA and aspartate increases expenditure adipocytes. However, exact mechanism how contributes to accelerated mobilization...
Abstract Considering the already established relationship between extent of metabolic dysfunction and severity heart failure (HF), it is conceivable that metabolomic profile serum may have a prognostic capacity for 3-month mortality in acute (AHF). Out 152 recruited patients, 130 samples were subjected to analyses. The rate was 24.6% (32 patients). Metabolomic profiling by nuclear magnetic resonance spectroscopy found levels 2-hydroxybutyrate (2-HB), 3-hydoxybutyrate (3-HB), lactate,...
Human life expectancy continues to grow globally, and so does the prevalence of age-related chronic diseases, causing a huge medical economic burden on society. Effective therapeutic options for these disorders are scarce, even if available, typically limited single comorbidity in multifaceted dysfunction that inevitably affects all organ systems. Thus, novel therapies target fundamental processes aging itself desperately needed. In this article, we summarize current strategies successfully...
A novel in vitro test (T50 test) assesses ex vivo serum calcification propensity and predicts mortality chronic kidney disease haemodialysis (HD) patients. For the latter, a time-dependent decline of T50 was shown to relate mortality. Here we assessed whether 3-month switch acetate-free, citrate-acidified, standard bicarbonate HD (CiaHD) sustainably improves propensity.T50 values were paired midweek pre-dialysis sera collected before 3 months after CiaHD 78 prevalent European In all, 44 then...
In pancreatic β-cells, the intracellular Ca²⁺ homeostasis is an essential regulator of cells major functions. The endoplasmic reticulum (ER) as interactive store balances cellular Ca²⁺. this study basal ER was evaluated in order to reveal potential β-cell-specificity handling and its consequences for mitochondrial Ca²⁺, ATP respiration.The two cell lines INS-1 MIN-6, freshly isolated islets, non-pancreatic HeLA EA.hy926 were used. Cytosolic, measurements performed using single fluorescence...
Research and development are facilitated by sharing knowledge bases, the innovation process benefits from collaborative efforts that involve collective utilization of data. Until now, most companies organizations have produced collected various types data, stored them in data silos still to be integrated with one another order enable creation. For this happen, both public private actors must adopt a flexible approach achieve necessary transition break create between producers users. In...
Abstract CyVerse, the largest publicly-funded open-source research cyberinfrastructure for life sciences, has played a crucial role in advancing data-driven since 2010s. As technology landscape evolved with emergence of cloud computing platforms, machine learning and artificial intelligence (AI) applications, CyVerse enabled access by providing interfaces, Software as Service (SaaS), cloud-native Infrastructure Code (IaC) to leverage new technologies. services enable researchers integrate...
Increased intestinal or gastric permeability is one of the major hallmarks liver cirrhosis. The current gold standard for diagnosis aberrant gut due to disease triple-sugar test, where carbohydrates are orally administered and urinary excretion measured. Hereby, elevated lactulose levels indicate permeability, whereas increased sucrose reveal permeability. However, reliable detection quantification these sugars in a complex biological fluid still remains challenging interfering substances....