A5091673111- Nitric Oxide and Endothelin Effects
- Genomics, phytochemicals, and oxidative stress
- Hormonal Regulation and Hypertension
- Cardiac Ischemia and Reperfusion
- Glutathione Transferases and Polymorphisms
- Cardiovascular Health and Disease Prevention
- Cardiovascular Disease and Adiposity
- Transplantation: Methods and Outcomes
- Cardiac electrophysiology and arrhythmias
- Mechanical Circulatory Support Devices
- Receptor Mechanisms and Signaling
- Renin-Angiotensin System Studies
- Cardiovascular Function and Risk Factors
- Blood Pressure and Hypertension Studies
- Atherosclerosis and Cardiovascular Diseases
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Eicosanoids and Hypertension Pharmacology
- Acute Kidney Injury Research
- Electrolyte and hormonal disorders
- Thermoregulation and physiological responses
- Apelin-related biomedical research
- Histone Deacetylase Inhibitors Research
- Ion channel regulation and function
- Arsenic contamination and mitigation
- Selenium in Biological Systems
Servier (France)
2007-2019
Inserm
1998-2016
Sorbonne Université
2016
Hôpital Paris Saint-Joseph
2016
Université Paris Cité
2016
Institut Cardiovasculaire Paris Sud
2003-2014
University of Arizona
2008-2013
Institut Universitaire de Cardiologie et de Pneumologie de Québec
2013
Université Laval
2013
D'Youville College
2011
AbstractIn response to stress, cells can utilize several cellular processes, such as autophagy, which is a bulk-lysosomal degradation pathway, mitigate damages and increase the chances of cell survival. Deregulation autophagy causes upregulation p62 formation p62-containing aggregates, are associated with neurodegenerative diseases cancer. The Nrf2-Keap1 pathway functions critical regulator cell's defense mechanism against oxidative stress by controlling expression many protective proteins....
Drug resistance during chemotherapy is the major obstacle to successful treatment of many cancers. Here, we report that inhibition NF-E2-related factor 2 (Nrf2) may be a promising strategy combat chemoresistance. Nrf2 critical transcription regulating cellular protective response defends cells against toxic insults from broad spectrum chemicals. Under normal conditions, low constitutive amount protein maintained by Kelch-like ECH-associated protein1 (Keap1)-mediated ubiquitination and...
The major obstacle in cancer treatment is the resistance of cells to therapies. Nrf2 a transcription factor that regulates cellular defense response and ubiquitously expressed at low basal levels normal tissues due Keap1-dependent ubiquitination proteasomal degradation. Recently, has emerged as an important contributor chemoresistance. High constitutive expression was found many types cancers, creating environment conducive for cell survival. Here, we report identification brusatol unique...
Abstract The discoveries made in the 1980s that NO could be synthesized by mammalian cells and act as physiological messenger cytotoxic agent had elevated importance of its detection. numerous properties NO, enable it to carry out diverse functions, also present considerable problems when attempting detection quantification biological systems. Indeed, total free concentration conditions has been established nanomolar range. Thus, nitric oxide remains a challenge, pointing difficult dual...
Colorectal cancer (CRC) is a major cause of tumor-related morbidity and mortality worldwide. Recent research suggests that pharmacological intervention using dietary factors activate the redox sensitive Nrf2/Keap1-ARE signaling pathway may represent promising strategy for chemoprevention human including CRC. In our search Nrf2 activators with potential chemopreventive activity targeting CRC, we have focused studies on trans-cinnamic aldehyde (cinnamaldeyde, CA), key flavor compound in...
Ivabradine, a specific and use-dependent I(f) inhibitor, exerts anti-ischaemic activity purely by reducing heart rate. The aim of this work was to characterize its effect on the predominant HCN channel isoform expressed in human sino-atrial nodes (hSAN), determine kinetics channels from multicellular preparations rate-dependency action.RT-PCR analysis four isoforms carried out RNAs hSAN. Patch-clamp intracellular recordings were obtained CHO cells stably expressing hHCN4 isolated SAN,...
Groundwater contaminated with arsenic imposes a big challenge to human health worldwide. Using natural compounds subvert the detrimental effects of represents an attractive strategy. The transcription factor nuclear erythroid 2-related 2 (Nrf2) is critical regulator cellular antioxidant response and xenobiotic metabolism. Recently, activation Nrf2 signaling pathway has been reported confer protection against arsenic-induced toxicity in cell culture model.The goal present work was identify...
Senescence is characterized by a gradual decline in cellular functions, including changes energy homeostasis and decreased proliferation activity. As power plants, contributors to signal transduction, sources of reactive oxygen species (ROS) executors programmed cell death, mitochondria are unique position affect aging-associated processes decline. Notably, metabolic activation tightly linked Ca2+ due the -dependency several enzymes Krebs cycle, however, overload with triggers death...
Lipocalin-2 is a proinflammatory adipokine upregulated in obese humans and animals. A pathogenic role of lipocalin-2 hypertension has been suggested. Mice lacking are protected from dietary obesity-induced cardiovascular dysfunctions. Administration causes abnormal vasodilator responses mice on high-fat diet (HFD).Wild-type knockout were fed with standard chow or HFD. Immunoassays performed for evaluating the circulating tissue contents lipocalin-2. The relaxation contraction arteries...
Generous donors: The dithioperoxyanhydrides (CH3COS)2, (PhCOS)2, CH3COSSCO2Me and PhCOSSCO2Me act as thiol-activated hydrogen sulfide donors in aqueous buffer solution. most efficient donor (CH3COS)2 can induce a biological response cells, advantageously replace ex vivo vascular studies. As service to our authors readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed may be re-organized for online delivery, but not copy-edited or...
1. The purpose of these experiments was to determine whether or not the endothelium-dependent hyperpolarizations vascular smooth muscle cells (observed in presence inhibitors nitric oxide synthase and cyclo-oxygenase) can be attributed production an endogenous cannabinoid. 2. Membrane potential recorded guinea-pig carotid, rat mesenteric porcine coronary arteries by intracellular microelectrodes. 3. In artery, cannabinoid receptor antagonist, SR 141716 (1 microM), did modify either resting...
Age and hypertension are major causes of large artery remodeling stiffening, a cardiovascular risk factor for heart kidney damage. The aged spontaneously hypertensive rat (SHR) model is recognized human pathology, but discrepancies appeared in studies arterial stiffness. We performed experiments using robust analysis via echo tracking 20-week adult (n = 8) 80-week-old SHR 7), with age-matched normotensive Wistar Kyoto rats (WKY, n 6;6) at basal matched levels blood pressure (BP). After...
Lipocalin-2 is not only a sensitive biomarker, but it also contributes to the pathogenesis of renal injuries. The present study demonstrates that adipose tissue–derived lipocalin-2 plays critical role in causing both chronic and acute Four-week treatment with aldosterone high salt after uninephrectomy (ANS) significantly increased circulating urinary lipocalin-2, induced glomerular tubular injuries kidneys WT mice. Despite expression lcn2 excretion mice selective deletion alleles tissue...
Hyperlipidemia may increase endothelial damage and promote accelerated atherogenesis in graft coronary vasculopathy. To study the effects of hypercholesterolemia on dysfunction, intimal hyperplasia, lipid content, a porcine model heterotopic heart transplantation, allowing nonacute rejection without immunosuppressive drugs, was used. A high cholesterol diet fed to donor recipient swine 1 month before after transplantation. The function arteries native transplanted hearts from cholesterol-fed...
The present study was designed to test the ability of regenerated endothelium evoke endothelium-dependent hyperpolarizations. Hyperpolarizations induced by serotonin and bradykinin were compared in isolated porcine coronary arteries with native or endothelium, 4 weeks after balloon endothelial denudation. experiments performed presence inhibitors nitric oxide synthase (Nomega-nitro-L-arginine) cyclooxygenase (indomethacin). transmembrane potential measured using conventional glass...
Experiments were designed to investigate whether the pertussis toxin‐dependent endothelial dysfunction following balloon injury is due a reduced expression or an insufficient function of G‐proteins. Endothelium‐dependent responses porcine coronary arteries examined in vitro by use conventional organ chambers. Morphological analysis was performed isolating and culturing cells from these arteries. The Gi‐proteins regenerated measured Western blots immunolabelling. G‐proteins assessed measuring...