A5114375876- Fungal and yeast genetics research
- Fermentation and Sensory Analysis
- Horticultural and Viticultural Research
- Microbial Metabolic Engineering and Bioproduction
- RNA Research and Splicing
- Tea Polyphenols and Effects
- Biofuel production and bioconversion
- Fungal Biology and Applications
- Redox biology and oxidative stress
- Phytochemicals and Antioxidant Activities
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- Genetics, Aging, and Longevity in Model Organisms
- Polyamine Metabolism and Applications
- Autophagy in Disease and Therapy
- Ubiquitin and proteasome pathways
- RNA Interference and Gene Delivery
- Endoplasmic Reticulum Stress and Disease
- Meat and Animal Product Quality
- Wine Industry and Tourism
- Yeasts and Rust Fungi Studies
- Plant responses to water stress
- Biochemical and biochemical processes
- Intensive Care Unit Cognitive Disorders
- Tree Root and Stability Studies
Universitat de València
2015-2025
Institute for Integrative Systems Biology
2016-2025
Instituto de Agroquímica y Tecnología de Alimentos
2006-2018
Consejo Superior de Investigaciones Científicas
2003-2012
Universidad Autónoma de Madrid
2007
Unidades Centrales Científico-Técnicas
2003
University of Oxford
1999-2002
Tufts University
1998
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, on this topic has continued to accelerate, and many new scientists have entered field. Our knowledge base relevant technologies also been expanding. Accordingly, it is important update these monitoring autophagy different organisms. Various reviews described range assays that used purpose. Nevertheless, there continues be confusion regarding acceptable methods measure autophagy, especially...
Abstract Background Yeast viability and vitality are essential for different industrial processes where the yeast Saccharomyces cerevisiae is used as a biotechnological tool. Therefore, decline of biological functions during aging may compromise their successful use. Life span controlled by variety molecular mechanisms, many which connected to stress tolerance genomic stability, although metabolic status cell has proven main factor affecting its longevity. Acetic acid ethanol accumulation...
The nucleolus is the site of ribosome synthesis in nucleus, whose integrity essential. Epigenetic mechanisms are thought to regulate activity ribosomal RNA (rRNA) gene copies, which part nucleolus. Here we show that human cells lacking DNA methyltransferase 1 (Dnmt1), but not Dnmt33b, have a loss methylation and an increase acetylation level lysine 16 histone H4 at rRNA genes. Interestingly, observed SirT1, NAD+-dependent deacetylase with preference for H4, interacts Dnmt1; SirT1 recruitment...
Abstract One of the stress conditions that yeast may encounter is presence acetaldehyde. In a previous study we identified that, in response to this stress, several HSP genes are induced also involved other forms stress. Aldehyde dehydrogenases (ALDH) play an important role acetaldehyde metabolism (e.g. when cells growing ethanol). work analyse expression encoding these enzymes ( ALD ) and corresponding enzymatic activities under growth conditions. We investigate three kinds strains:...
Abstract The bioprospection of indigenous microorganism strains with biotechnological potential represents a prominent trend. Metschnikowia yeasts exhibit diverse capabilities, such as ethanol reduction in winemaking, biocontrol potential, and lipid production. In this work, local were isolated from different fruits by their ability to produce pulcherrimic acid, molecule that has been linked activity binds iron giving colored colonies. Five selected, each one five distinct sources. All them...
Acetic acid is a key metabolite in yeast fermentation, influencing wine quality through its role volatile acidity. In Saccharomyces cerevisiae, acetic production involves aldehyde dehydrogenases, primarily Ald6p during fermentation and Ald4p under respiratory conditions. However, the regulatory mechanisms of these enzymes throughout how they differ commonly used strains remain partially unclear. This study explores cytosolic peroxiredoxin Tsa1p as novel regulator metabolism. TSA1 gene...
ABSTRACT Acetaldehyde is a toxic compound produced by Saccharomyces cerevisiae cells under several growth conditions. The adverse effects of this molecule are important, as significant amounts accumulate inside the cells. By means global gene expression analyses, we have detected acetaldehyde addition in about 400 genes. Repressed genes include many involved cell cycle control, polarity, and mitochondrial protein biosynthesis machinery. Increased displayed stress response genes, well other...
Transcriptional termination of the GAL10 gene in Saccharomyces cerevisiae depends on efficiency polyadenylation. Either cis mutations poly(A) signal or trans mRNA 3′ end cleavage factors result read-through transcripts into adjacent GAL7 and inactivation (occlusion) promoter. Herein, we present a molecular explanation this transcriptional interference phenomenon. In vivo footprinting data reveal that promoter occlusion is associated with displacement Gal4p transcription from Interestingly,...
Most grape juice fermentation takes place when yeast cells are in a nondividing state called the stationary phase. Under such circumstances, we aimed to identify genetic determinants controlling longevity, known as chronological life span. We identified commercial strains with both short (EC1118) and long (CSM) spans laboratory growth medium compared them under diverse conditions. Strain CSM shows better tolerance stresses, including oxidative stress, This is reflected during winemaking,...
Saccharomyces cerevisiae chronological life span (CLS) is determined by a wide variety of environmental and genetic factors. Nutrient limitation without malnutrition, i.e. dietary restriction, expands CLS through the control nutrient signaling pathways, which TOR/Sch9 has proven to be most relevant, particularly under nitrogen deprivation. The use prototrophic wine yeast allows better understanding role in longevity natural more demanding environments, such as grape juice fermentation. We...
The bulk of grape juice fermentation is carried out by the yeast Saccharomyces cerevisiae, but non-Saccharomyces yeasts can modulate many sensorial aspects final products in ways not well understood. In this study, some such non-conventional were screened as mixed starter cultures a defined growth medium both simultaneous and sequential inoculations. One strain Starmerella bacillaris another Zygosaccharomyces bailii chosen their distinct phenotypic footprint ability to reduce ethanol levels...
Flor yeasts are a particular kind of Saccharomyces cerevisiae strains involved in Sherry wine biological ageing. During this process, form film on the surface and use ethanol as carbon source, producing acetaldehyde by-product. Acetaldehyde induces BTN2 transcription laboratory strains. Btn2p is control subcellular localization different proteins. The gene shows complex expression pattern yeast, increasing its by acetaldehyde, but repressing it ethanol. A flor yeast strain transcribes more...
Viability in a non dividing state is referred to as chronological life span (CLS). Most grape juice fermentation happens when Saccharomyces cerevisiae yeast cells have stopped dividing; therefore, CLS an important factor toward winemaking success.We studied both the physical and chemical determinants influencing CLS. Low pH heat shorten maximum wine span, while hyperosmotic shock extends it. Ethanol plays negative role aging under conditions, but additional metabolites produced by...