A5014254750- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Fungal and yeast genetics research
- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- RNA Research and Splicing
- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Plant Gene Expression Analysis
- Polyamine Metabolism and Applications
- RNA modifications and cancer
- Genetics and Neurodevelopmental Disorders
- Plant Reproductive Biology
- Protein Degradation and Inhibitors
- Yeasts and Rust Fungi Studies
- Plant Disease Resistance and Genetics
- Food Allergy and Anaphylaxis Research
- Biomarkers in Disease Mechanisms
- Chromatin Remodeling and Cancer
- Allergic Rhinitis and Sensitization
- Postharvest Quality and Shelf Life Management
- Entomological Studies and Ecology
- Inflammatory Bowel Disease
- Connexins and lens biology
Cardiff University
2009-2025
University College London
2023
Leiden University
2023
Vetenskap I Skolan
2023
Imperial College London
2007-2023
University of Minnesota System
2017
University of Exeter
2010
University of Oxford
1994-2007
LASALLE College of the Arts
2007
University of East Anglia
1988-1990
Microarray and next-generation sequencing techniques which allow whole genome analysis of chromatin structure sequence-specific protein binding are revolutionizing our view chromosome architecture function. However, many current methods in this field rely on biochemical purification highly specific fractions DNA prepared from digested with either micrococcal nuclease or DNaseI restricted the parameters they can measure. Here, we show that a broad size-range genomic species, produced by...
Isw1p and Isw2p are budding yeast homologs of the Drosophila ISWI chromatin-remodeling ATPase. Using indirect-end-label chromatin immunoprecipitation analysis, we show both independent cooperative Isw1p- Isw2p-mediated positioning short nucleosome arrays in gene-regulatory elements at a variety transcription units vivo. We present evidence that complexes regulate developmental responses to starvation for Isw2p, recruitment by different DNA-binding proteins controls meiosis haploid invasive growth.
The INO80 chromatin remodeling complex functions in transcriptional regulation, DNA repair, and replication. Here we uncover a novel role for regulating chromosome segregation. First, show that the conserved Ies6 subunit is critical function vivo. Strikingly, found loss of either or Ino80 catalytic results rapid increase ploidy. One route to polyploidy through missegregation due aberrant centromere structure, leads defective Importantly, structure flanking centromeres altered cells lacking...
Abstract In eukaryotes, histone paralogues form obligate heterodimers such as H3/H4 and H2A/H2B that assemble into octameric nucleosome particles. Archaeal histones are dimeric on DNA ‘hypernucleosome’ particles of varying sizes with each dimer wrapping 30 bp DNA. These composed canonical variant paralogues, but the function these variants is poorly understood. Here, we characterise structure paralogue MJ1647 from Methanocaldococcus jannaschii has a unique C-terminal extension enabling...
Abstract The Arabidopsis Knotted1 -like homeobox ( KNOX ) gene SHOOT MERISTEMLESS STM encodes a homeodomain transcription factor that operates as central component of the regulatory network (GRN) controlling shoot apical meristem formation and maintenance. It regulates expression target genes include transcriptional regulators associated with function, particularly those involved in pluripotency cellular differentiation, well hormone metabolism signaling. Previous studies have identified...
Glutathione (γ-l-glutamyl-l-cysteinylglycine) is an important antioxidant molecule, helping to buffer the cell against free radicals and toxic electrophiles. Expression of yeast<i>GSH1</i> gene, encoding first enzyme involved in glutathione biosynthesis, γ-glutamylcysteine synthetase, regulated by oxidants heavy metal cadmium at level transcription. We present evidence that transcription factors controlling network sulfur amino acid metabolism genes are also responsible for regulating...
DNA double strand breaks (DSBs) are potentially serious chromosomal lesions. However, cells sometimes deliberately cleave their own to facilitate certain processes, and there is much interest in how such self-inflicted effectively managed. Eukaryotic DSBs occur the context of chromatin RSC chromatin-remodeling ATPase complex has been shown promote DSB repair at budding yeast MAT locus DSB, created by HO endonuclease during mating type switching. We show that role highly specialized. The...
Budding yeast centromeres are sequence-defined point and are, unlike in many other organisms, not embedded heterochromatin. Here we show that Fun30, a poorly understood SWI/SNF-like chromatin remodeling factor conserved humans, promotes centromere function through the formation of correct architecture at centromeres. Our determination genome-wide binding nucleosome positioning properties Fun30 shows this enzyme is consistently enriched over majority CENs Fun30-dependent changes flanking...
All eukaryotic genomes are packaged as chromatin, with DNA interlaced both regularly patterned nucleosomes and sub-nucleosomal-sized protein structures such mobile labile transcription factors (TF) initiation complexes, together forming a dynamic chromatin landscape. Whilst details of nucleosome position in Arabidopsis have been previously analysed, there is less understanding their relationship to more sub-nucleosomal particles (subNSPs) defined protected regions shorter than the ~150bp...
Strawberry fruit are highly valued for their aroma which develops during ripening. However, they have a short shelf-life. Low temperature storage is routinely used to extend shelf-life transport and in the supply chain, however cold can also affect aroma. Some continue ripen chilled storage; however, strawberries non-climacteric hence ripening postharvest limited. Although most strawberry sold whole, halved ready eat fresh salads of increasing consumer demand pose additional challenges storage.
Cbf1p is a basic-helix-loop-helix-zipper protein of Saccharomyces cerevisiae required for the function centromeres and MET gene promoters, where it binds DNA via consensus core motif CACRTG (R = A or G). At genes appears to in both activator recruitment chromatin-remodeling. has been implicated regulation other genes, motifs are common potential regulatory DNA. recent genome-wide location analysis showed that majority intergenic CACGTG palindromes bound by Cbf1p. Here we tested whether all...
Using the polymerase chain reaction (PCR) we have amplified and cloned genomic DNA encoding secreted group I allergen proteins from house dust mite species Euroglyphus maynei, Dermatophagoides pteronyssinus D. farinae. Affinity chromatography using a monoclonal antibody to Der p was used purify protein E. maynei. We present deduced amino acid sequence of new member family Eur m I. The three show high level primary structure similarity: 85% identity, sequences taken together 78% identity. A...
The RSC chromatin remodeling complex has been implicated in contributing to DNA double-strand break (DSB) repair a number of studies. Both survival and levels H2A phosphorylation response damage are reduced the absence RSC. Importantly, there is evidence for two isoforms this complex, defined by presence either Rsc1 or Rsc2. Here, we investigated whether provide distinct contributions responses. First, established that differ Rsc2 but otherwise have same subunit composition. We found both...
Journal Article Chromatin analysis in yeast using NP-40 permeabilised sphaeroplasts Get access Nicholas A. Kent, Kent Microbiology Unit, Department of Biochemistry, University OxfordSouth Parks Road, Oxford 0X1 3QU, UK Search for other works by this author on: Academic PubMed Google Scholar Louise E. Bird, Bird Jane Mellor Nucleic Acids Research, Volume 21, Issue 19, 25 September 1993, Pages 4653–4654, https://doi.org/10.1093/nar/21.19.4653 Published: 1993 history Received: 22 June Revision...
Forkhead (Fkh) transcription factors influence cell death, proliferation, and differentiation the cycle. In Saccharomyces cerevisiae, Fkh2 both activates represses of CLB2, encoding a B-type cyclin. CLB2 is expressed during G(2)/M phase repressed G(1). recruits coactivator Ndd1, an interaction which promoted by Clb2/Cdk1-dependent phosphorylation suggesting that autoregulated. Ndd1 proposed to function antagonizing Fkh2-mediated repression, but nothing known about mechanism. Here we ask how...
In Saccharomyces cerevisiae genes that are located close to a telomere can become transcriptionally repressed by an epigenetic process known as position effect. There is large variation in the level of effect among telomeres, with many native ends exhibiting little repression.Chromatin analysis, using microccocal nuclease and indirect end labelling, reveals distinct patterns for different silencing states. Differences were observed promoter accessibility subtelomeric reporter gene...
Article18 November 2015Open Access Source Data Abo1, a conserved bromodomain AAA-ATPase, maintains global nucleosome occupancy and organisation Csenge Gal Institute for Cell & Molecular Biosciences, Newcastle University, Newcastle, UK Search more papers by this author Heather E Murton Lakxmi Subramanian Wellcome Trust Centre Biology of Biology, University Edinburgh, Alex J Whale Karen M Moore College Life Environmental Sciences, Exeter, Konrad Paszkiewicz Sandra Codlin Department Genetics,...
CPF1 is an abundant basic-helix-loop-helix-ZIP protein that binds to the CDEI motif in Saccharomyces cerevisiae centromeres and promoters of numerous genes, including those encoding enzymes methionine biosynthetic pathway. Strains lacking are auxotrophs, it has been proposed might positively influence transcription at MET25 MET16 genes by modulating promoter chromatin structure. We test this hypothesis show regions surrounding motifs maintained a nucleosome-free state requires entire...