A5065148464- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Fungal and yeast genetics research
- RNA modifications and cancer
- Enzyme Catalysis and Immobilization
- Legume Nitrogen Fixing Symbiosis
University of Oxford
2007-2012
University of Parma
2003-2008
Both RNA polymerase I and II (Pol Pol II) in budding yeast employ a functionally homologous “torpedo-like” mechanism to promote transcriptional termination. For two well-defined II-transcribed genes, CYC1 PMA1 , we demonstrate that both Rat1p exonuclease Sen1p helicase are required for efficient termination by promoting degradation of the nascent transcript associated with II, following mRNA 3′ end processing. Similarly, relies on prior Rnt1p cleavage at pre-rRNA 35S transcript. This is...
Eukaryotic RNA polymerase (Pol) III terminates transcription at short runs of T residues in the coding DNA strand. By genomic analysis, we found that T(5) and T(4) are shortest Pol termination signals yeasts mammals, respectively, that, variance with yeast, oligo(dT) terminators longer than very rare mammals. In Saccharomyces cerevisiae, strength as a terminator was to be largely influenced by both upstream downstream sequence context. particular, CT sequence, which is naturally present...
A key event in tRNA gene (tDNA) transcription by RNA polymerase (Pol) III is the TFIIIC-dependent assembly of TFIIIB upstream start site. Different tDNA sequences bind with different affinities, thereby modulating transcription. We found that absence Nhp6 proteins, influence 5′-flanking region on dramatically enhanced Saccharomyces cerevisiae. Expression a bearing suboptimal binding site, but not preceded strong region, was strongly dependent vivo. Upstream sequence-dependent stimulation...
Ribosomal RNA, transcribed by RNA polymerase (Pol) I, accounts for most cellular RNA. Since Pol I transcribes rDNA repeats with high processivity and density, transcription termination is a critical process. Early in vitro studies proposed pausing Reb1 transcript release at the T-rich element T1 determined termination. However recent vivo revealed 'torpedo' mechanism termination: co-transcriptional cleavage Rnt1 provides an entry site 5′–3′ exonuclease Rat1 that degrades I-associated...