A5051964998- Erythrocyte Function and Pathophysiology
- Autophagy in Disease and Therapy
- Biochemical effects in animals
- Heavy Metal Exposure and Toxicity
- Blood properties and coagulation
- Neurological Disease Mechanisms and Treatments
- Free Radicals and Antioxidants
- Natural product bioactivities and synthesis
- Barrier Structure and Function Studies
- Phagocytosis and Immune Regulation
- Ginseng Biological Effects and Applications
- Angiogenesis and VEGF in Cancer
- Neuroscience and Neuropharmacology Research
- Phytochemistry and Biological Activities
- Traditional Chinese Medicine Analysis
- Trace Elements in Health
- Antioxidant Activity and Oxidative Stress
- Heme Oxygenase-1 and Carbon Monoxide
- Arsenic contamination and mitigation
- Cannabis and Cannabinoid Research
- Pharmacological Effects of Natural Compounds
- Platelet Disorders and Treatments
- Antiplatelet Therapy and Cardiovascular Diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced Glycation End Products research
Hanyang University
2016-2025
Organ Technologies (Japan)
2024
Konkuk University
2024
Anyang University
2017-2024
BAE Systems (Sweden)
2015
Ajou University
2006-2014
Michigan State University
2008-2014
Daegu Gyeongbuk Institute of Science and Technology
2014
University of Sheffield
2014
University of Pittsburgh
2013
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, on this topic has continued to accelerate, and many new scientists have entered field. Our knowledge base relevant technologies also been expanding. Accordingly, it is important update these monitoring autophagy different organisms. Various reviews described range assays that used purpose. Nevertheless, there continues be confusion regarding acceptable methods measure autophagy, especially...
Although erythrocytes have been suggested to play a role in blood clotting, mediated through phosphatidylserine (PS) exposure and/or PS-bearing microvesicle generation, an endogenous substance that triggers the membrane alterations leading procoagulant activity has not reported. We now demonstrated lysophosphatidic acid (LPA), important lipid mediator various pathophysiological processes, induces PS and generation erythrocytes, which represent biological significance resulting induction of...
Despite the rapidly increasing global burden of ischemic stroke, no therapeutic options for neuroprotection against stroke currently exist. Recent studies have shown that autophagy plays a key role in neuronal death, and treatments target may represent novel strategy neuroprotection. We investigated whether is regulated by carnosine, an endogenous pleiotropic dipeptide has robust neuroprotective activity brain damage.We examined effect carnosine on mitochondrial dysfunction autophagic...
Despite the wide use of silver nanoparticles (nano Ag), its toxicity still remains poorly understood. In this report, nano Ag induced an increase in platelet aggregation and procoagulant activation which are key contributors to thrombotic diseases. freshly isolated human platelets, evident by increased phosphatidylserine exposure thrombin generation. Interestingly, sub-threshold level enhanced Ag-induced significantly indicating that prothrombotic effects might be further potentiated...
The blood-brain barrier (BBB) maintains homeostasis of the brain environment by tightly regulating entry substances from systemic circulation. A breach in BBB results increased permeability to potentially toxic and is an important contributor amplification ischemic damage. precise molecular pathways that result impairment integrity remain be elucidated. Autophagy a degradation pathway clears damaged or unnecessary proteins cells. However, excessive autophagy can lead cellular dysfunction...
Background: The purpose of this study was to develop a novel silymarin-loaded solid nanoparticle system with enhanced oral bioavailability and an ability provide excellent hepatic protection for poorly water-soluble drugs using Shirasu porous glass (SPG) membrane emulsification spray-drying technique. Methods: A liquid nanoemulsion formulated by applying the SPG This further converted into state nanosized particles physicochemical characteristics these nanoparticles were determined scanning...
Background and Purpose— An urgent need exists to develop therapies for stroke that have high efficacy, long therapeutic time windows, acceptable toxicity. We undertook preclinical investigations of a novel approach involving supplementation with carnosine, an endogenous pleiotropic dipeptide. Methods— Efficacy safety carnosine treatment was evaluated in rat models permanent or transient middle cerebral artery occlusion. Mechanistic studies used primary neuronal/astrocytic cultures ex vivo...
Cardiovascular complications are the major causes of death in patients with diabetes mellitus. Several studies have demonstrated that endothelial progenitor cells (EPCs), adult stem contributing to regeneration vascular endothelium, dysfunctional under diabetic condition resulting impaired peripheral circulation and delayed wound healing. In this study, we investigated cellular alteration EPCs high glucose condition, elucidate mechanisms underlying diabetes-associated EPC dysfunction. were...
Abstract Background Microplastics (MPs) and nanoplastics (NPs) formed from decomposed plastic are increasing environmental threats. Although MPs NPs exposed through various routes enter the systemic circulation, potential toxicity of those is largely unknown. We investigated whether polystyrene (PS-NPs) promote coagulation activity red blood cells (RBCs). Results tested several types PS-NPs using human RBCs found that amine-modified 100 nm were most potent. measured uptake flow cytometry...
The gut microbiota affects hepatic drug metabolism. However, microbial factors modulating metabolism are largely unknown. In this study, using a mouse model of acetaminophen (APAP)-induced hepatotoxicity, we identified bacterial metabolite that controls the expression CYP2E1 catalyzes conversion APAP to reactive, toxic metabolite. By comparing C57BL/6 substrain mice from two different vendors, Jackson (6J) and Taconic (6N), which genetically similar but harbor microbiotas, established...
BackgroundAssociations between cardiovascular diseases and mercury have been frequently described, but underlying mechanisms are poorly understood.ObjectivesWe investigate the procoagulant activation of erythrocytes, an important contributor to thrombosis, by low-level explore roles erythrocytes in mercury-related diseases.MethodsWe used freshly isolated human ex vivo thrombosis models rats mercury-induced activity.ResultsProlonged exposure low-dose mercuric ion (Hg2+; 0.25–5 μM for 1–48 hr)...
Silver nanoparticles (AgNP) are widely used in medical practices owing to their distinct antibacterial, antiviral and anticancer activities. However, with increasing use of AgNP, concerns over its potential toxicity also escalating. Here, we demonstrated the thrombotic effect AgNP which was mediated by procoagulant activity red blood cells (RBCs). In freshly isolated human RBCs, but not silver microparticles (AgMP), elicited morphological changes, phosphatidylserine (PS) exposure...