A5038996345- Immune Cell Function and Interaction
- Viral Infections and Vectors
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Vector-Borne Animal Diseases
- Viral Infections and Outbreaks Research
- Carbohydrate Chemistry and Synthesis
- Fire effects on ecosystems
- T-cell and Retrovirus Studies
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Animal Disease Management and Epidemiology
- Fluorine in Organic Chemistry
- Glycosylation and Glycoproteins Research
- Acute Myeloid Leukemia Research
- Mosquito-borne diseases and control
- Cytomegalovirus and herpesvirus research
- Synthesis and Reactions of Organic Compounds
- Synthesis of β-Lactam Compounds
- Autophagy in Disease and Therapy
- Amino Acid Enzymes and Metabolism
- RNA Interference and Gene Delivery
- PI3K/AKT/mTOR signaling in cancer
- Polyamine Metabolism and Applications
- Virus-based gene therapy research
Cincinnati Children's Hospital Medical Center
2022-2025
University of Cincinnati
2020-2025
University of Cincinnati Medical Center
2022-2025
Kyoto Sangyo University
2025
Emory University
2012-2023
Children’s Institute
2020-2023
Emory Healthcare
2010-2015
Emory and Henry College
2012
Oglethorpe University
2010-2011
Hokkaido University
1998-2009
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, on this topic has continued to accelerate, and many new scientists have entered field. Our knowledge base relevant technologies also been expanding. Accordingly, it is important update these monitoring autophagy different organisms. Various reviews described range assays that used purpose. Nevertheless, there continues be confusion regarding acceptable methods measure autophagy, especially...
Immunotherapeutic PD-1–targeted therapies require CD28 to promote cancer cell killing.
Inhibitory receptors play a crucial role in regulating CD8 T-cell function during chronic viral infection. Ig- and mucin-domain–containing molecule–3 (Tim-3) is well known to negatively regulate responses, but its exhaustion infection vivo remains unclear. In this study, we document coregulation of T cell by Tim-3 PD-1 lymphocytic choriomeningitis virus Whereas was only transiently expressed cells after acute infection, virus-specific retained high expression throughout The majority...
PD-1 (programmed cell death-1) is the central inhibitory receptor regulating CD8 T exhaustion during chronic viral infection and cancer. Interestingly, also expressed transiently by activated cells acute infection, but role of in modulating effector differentiation function not well defined. To address this question, we examined expression kinetics lymphocytic choriomeningitis virus (LCMV) mice. was rapidly up-regulated vivo upon activation naive virus-specific within 24 h after LCMV less...
T cell dysfunction is an important feature of many chronic viral infections. In particular, it was shown that programmed death-1 (PD-1) regulates during lymphocytic choriomeningitis virus infection in mice, and PD-1(hi) cells exhibit intense exhausted gene signature. These findings were extended to human infections such as HIV, hepatitis C virus, B virus. However, not known if healthy humans have the traits cells. this study, we provide a comprehensive description phenotype, function,...
Regulatory T (T reg) cells are critical for preventing autoimmunity mediated by self-reactive cells, but their role in modulating immune responses during chronic viral infection is not well defined. To address this question and to investigate a reg exhaustion of virus-specific CD8 we depleted mice chronically infected with lymphocytic choriomeningitis virus (LCMV). cell ablation resulted 10–100-fold expansion functional LCMV-specific cells. Rescue exhausted was dependent on cognate antigen,...
This phase II trial was performed to evaluate the efficacy of a new granulocyte colony‐stimulating factor (G‐CSF)‐supported multi‐agent chemotherapy protocol, LSG15, for aggressive adult T‐cell leukaemia‐lymphoma (ATL). Ninety‐six previously untreated patients with ATL were enrolled and grouped as: acute type (58), lymphoma (28) unfavourable chronic (10). Therapy consisted seven cycles VCAP (vincristine, cyclophosphamide, doxorubicin prednisone), AMP (doxorubicin, ranimustine prednisone)...
MicroRNAs are important regulators of various developmental and physiological processes. However, their roles in the CD8 + T-cell response not well understood. Using an acute viral infection model, we show that microRNAs miR-17-92 cluster strongly induced after activation, down-regulated clonal expansion, further silenced during memory development. promotes cell-cycle progression effector T cells, its expression is critical to rapid expansion these cells. excessive enhances mammalian target...
T cell exhaustion is a state of dysfunction associated with expression programmed death 1 (PD-1). Exhausted CD8+ cells are maintained by self-renewing stem-like that provide differentiated TIM3+ cells, part which possesses effector-like properties. PD-1-targeted therapies enhance response promoting differentiation toward but the role mTOR during remains elusive. Here, we showed inhibition has distinct outcomes beginning and after establishment chronic viral infection. Blocking expansion...
PD-1 + TCF-1 stem-like CD8 T cells act as critical resource for maintaining cell immunity in chronic viral infections and cancer. In addition, they provide the proliferative burst of effector after programmed death protein 1 (PD-1)–directed immunotherapy. However, it is not known whether checkpoint blockade diminishes number these progenitor differentiation increases. To investigate this, we used mouse model lymphocytic choriomeningitis virus (LCMV) infection. Treatment chronically infected...
Abstract Recent evidence demonstrating that exposure to rapamycin during viral infection increased the quantity and quality of Ag-specific T cells poses an intriguing paradox, because is used in transplantation dampen, rather than enhance, donor-reactive cell responses. In this report, we compared effects on response a bacterial versus transplant. Using transgenic system which Ag responding population were identical both cases, observed treatment with augmented pathogen, whereas it failed do...