A5027263129- Plant tissue culture and regeneration
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Seed Germination and Physiology
- Cellular transport and secretion
- Plant Taxonomy and Phylogenetics
- Plant Micronutrient Interactions and Effects
- Light effects on plants
- Plant Pathogens and Fungal Diseases
- Plant Virus Research Studies
- Transgenic Plants and Applications
- Legume Nitrogen Fixing Symbiosis
- Calcium signaling and nucleotide metabolism
- Horticultural and Viticultural Research
- Plant-Microbe Interactions and Immunity
- Plant Physiology and Cultivation Studies
- Chromatin Remodeling and Cancer
- Flowering Plant Growth and Cultivation
- Hippo pathway signaling and YAP/TAZ
- Cancer Mechanisms and Therapy
- Polysaccharides and Plant Cell Walls
- Aluminum toxicity and tolerance in plants and animals
Institute of Botany of the Slovak Academy of Sciences
2016-2024
Slovak Academy of Sciences
1992-2024
Comenius University Bratislava
1997-2020
Max Planck Institute for Plant Breeding Research
2002-2016
University of Bonn
2007-2013
Leibniz Institute of Plant Genetics and Crop Plant Research
2011-2013
Bayer (Belgium)
2011
Wageningen University & Research
2008
Max Planck Society
2001-2005
Pharmac
1992
Summary Δ‐1‐pyrroline‐5‐carboxylate synthetase enzymes, which catalyse the rate‐limiting step of proline biosynthesis, are encoded by two closely related P5CS genes in Arabidopsis. Transcription is differentially regulated drought, salinity and abscisic acid, suggesting that these play specific roles control biosynthesis. Here we describe genetic characterization p5cs insertion mutants, indicates P5CS1 required for accumulation under osmotic stress. Knockout mutations result reduction...
Plasma membrane repair in animal cells uses synaptotagmin 7, a Ca(2+)-activated fusion protein that mediates delivery of intracellular membranes to wound sites by mechanism resembling neuronal Ca(2+)-regulated exocytosis. Here, we show loss function the homologous Arabidopsis thaliana Synaptotagmin 1 (SYT1) reduces viability as consequence decrease integrity plasma membrane. This reduced is enhanced syt1-2 null mutant conditions osmotic stress likely caused defective repair. Consistent with...
Under blue light (BL) illumination, Arabidopsis thaliana roots grow away from the source, showing a negative phototropic response. However, mechanism of root phototropism is still unclear. Using noninvasive microelectrode system, we showed that BL sensor phototropin1 (phot1), signal transducer NONPHOTOTROPIC HYPOCOTYL3 (NPH3), and auxin efflux transporter PIN2 were essential for BL-induced flux in apex transition zone. We also found PIN2-green fluorescent protein (GFP) localized to...
Plants, as sessile organisms, are subjected to diverse abiotic stresses, including salinity, desiccation, metal toxicity, thermal fluctuations, and hypoxia at different phases of plant growth. Plants can activate messenger molecules initiate a signaling cascade response toward environmental stresses that results in either cell death or acclimation. Nitric oxide (NO) is small gaseous redox-active molecule exhibits plethora physiological functions growth, development, flowering, senescence,...
Abstract SWITCH/SUCROSE NONFERMENTING (SWI/SNF) chromatin-remodeling complexes mediate ATP-dependent alterations of DNA–histone contacts. The minimal functional core conserved SWI/SNF consists a SWI2/SNF2 ATPase, SNF5, SWP73, and pair SWI3 subunits. Because early duplication the gene family in plants, Arabidopsis thaliana encodes four SWI3-like proteins that show remarkable diversification. Whereas ATSWI3A ATSWI3B form homodimers heterodimers interact with BSH/SNF5, ATSWI3C, flowering...
Summary To assist in the analysis of plant gene functions we have generated a new Arabidopsis insertion mutant collection 90 000 lines that carry T‐DNA Agrobacterium fusion vector pPCV6NFHyg. Segregation indicates average frequency sites is 1.29 per line, predicting about 116 100 independent tagged loci collection. The copy number estimated by Southern DNA hybridization 2.4, as over 50% contain tandem copies. pooled two arrays providing 40 PCR templates, each containing from either 4000 or...
By using the photoconvertible fluorescence protein Dendra2 as a tag we demonstrated that neither naturally occurring auxins indole-3-acetic acid and indole-3-butyric acid, nor synthetic auxin analogs 1-naphthaleneacetic 2,4-dichlorophenoxyacetic compounds inhibiting polar transport such 2,3,5-triiodobenzoic 1-N-naphthylphthalamic were able to inhibit endocytosis of putative transporter PIN-FORMED2 (PIN2) in Arabidopsis (Arabidopsis thaliana) root epidermis cells. All compounds, except...
The mechanistic basis by which boron (B) deprivation inhibits root growth via the mediation of apical auxin transport and distribution remains elusive. This study showed that B repressed wild-type Arabidopsis seedlings, was related to higher accumulation (observed with DII-VENUS DR5-GFP lines) in B-deprived roots. Boron elevated content apex, coinciding upregulation expression levels biosynthesis-related genes (TAA1, YUC3, YUC9, NIT1) shoots, but not apices. Phenotyping experiments using...
Summary Epitope tagging provides a useful tool for immunological detection and cellular localization of proteins in vivo . Using T‐DNA‐mediated transformation, the epitope‐tagged planta is currently feasible only transgenic plants, because an artificial expression cDNA gene constructs driven by plant promoters bacteria obscures early Agrobacterium ‐infected cells. We have developed method labelling coding sequences with intron‐tagged epitope‐coding domains that are not processed Here we show...
Adventitious root formation in vitro 1-mm stem slices cut from microshoots of apple cv. Jork 9 was studied using light and electron microscopy. When indole-3-butyric acid (IBA) had been added to the medium, starch grains accumulated during first 24 h culture cells cambial region vicinity vascular tissue primary rays. This accumulation occurred only basal part explants. After that, nuclei these were activated, density cytoplasm number cell organelles increased, whereas broken down. Cambium...
The steady state level of integral membrane proteins is dependent on a strictly controlled delivery and removal. Here we show that Dendra2, green-to-red photoconvertible fluorescent protein, suitable tool to study protein turnover in plants. We characterized the fluorescence properties Dendra2 expressed either as free or tag Arabidopsis thaliana roots optimized photoconversion settings turnover. was fused PIN2 an auxin transporter root tip, by time-lapse imaging assessment red green signal...
We have examined the effect of medium-pH on rooting using 1-mm slices cut from stems apple microshoots. Before autoclaving, pH medium was set at various values between 4.5 and 8.0. During drifted in particular alkaline region. Additional changes occurred during culture range 4.5–8.0 had shifted to 5.2–6.0 after autoclaving 3 weeks culture. When 10 mM 2-(N-morpholino)ethanesulfonic acid (MES) been added as buffering agent, stable when 5.0–6.5. Highest achieved ~5.3 with without MES (pH...
ABSTRACT We examined the role of cytokinins in rooting 1-mm stem slices cut from microcuttings apple rootstock 'Jork 9˚s. Various types inhibited to different extents. Highest inhibition was obtained with thidiazuron and benzylaminopurine. Remarkably, isopentenyladenine isopentenyladenosine enhanced at low concentration (at optimal 0.1 μM by 53 19%, respectively). also effect lovastatin simvastatin. These drugs are putative cytokinin-synthesis inhibitors. Both partially reversed simultaneous...