A5009745090- Plant Stress Responses and Tolerance
- Polyamine Metabolism and Applications
- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Advanced Proteomics Techniques and Applications
- Legume Nitrogen Fixing Symbiosis
- Mass Spectrometry Techniques and Applications
- Prostate Cancer Treatment and Research
- Biosensors and Analytical Detection
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Phytochemical compounds biological activities
- Light effects on plants
- Moringa oleifera research and applications
- Machine Learning in Bioinformatics
- Electrochemical Analysis and Applications
- GABA and Rice Research
- Epigenetics and DNA Methylation
- Plant Reproductive Biology
- Phytochemistry Medicinal Plant Applications
- Biological Activity of Diterpenoids and Biflavonoids
- Plant and soil sciences
- Plant responses to water stress
Codexis (United States)
2024
Universitat de Barcelona
2004-2011
Instituto de Fisiología Vegetal
2009-2011
Center for Research in Agricultural Genomics
2009-2010
Consejo Superior de Investigaciones Científicas
2009
Centro de Investigaciones Biológicas Margarita Salas
2008
Universitat de València
2008
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2008
Consejo Nacional de Investigaciones Científicas y Técnicas
2004
National University of General San Martín
2004
The levels of endogenous polyamines have been shown to increase in plant cells challenged with low temperature; however, the functions regulation cold stress responses are unknown. Here, we show that accumulation putrescine under is essential for proper acclimation and survival at freezing temperatures because Arabidopsis (Arabidopsis thaliana) mutants defective biosynthesis (adc1, adc2) display reduced tolerance compared wild-type plants. Genes ADC1 ADC2 different transcriptional profiles...
Limited information is available concerning plant signal transduction pathways regulating polyamine (PA) metabolism in response to various abiotic stress conditions. To gain a new insight into this question, we have studied the possible role of abscisic acid (ABA) modulation PA under water conditions model Arabidopsis thaliana . Transcriptome profiling PA‐biosynthetic gene family by real‐time quantitative reverse transcriptase polymerase chain reaction revealed that arginine decarboxylase 2...
SignificanceDeep profiling of the plasma proteome at scale has been a challenge for traditional approaches. We achieve superior performance across dimensions precision, depth, and throughput using panel surface-functionalized superparamagnetic nanoparticles in comparison to conventional workflows deep proteomics interrogation. Our automated workflow leverages competitive nanoparticle-protein binding equilibria that quantitatively compress large dynamic range proteomes an accessible scale....
Summary We have obtained Arabidopsis thaliana transgenic plants constitutively overexpressing ADC2 , one of the two genes encoding arginine decarboxylase (ADC) in Arabidopsis. These contained very high levels putrescine (Put) but no changes were observed spermidine and spermine contents. The results from quantification free conjugated polyamines suggest that conjugation may be a limiting step for control Put homeostasis within non‐toxic range plant survival. Transgenic with increased...
Polyamines have been globally associated to plant responses abiotic stress. Particularly, putrescine has related a better response cold and dehydration stresses. It is known that this polyamine involved in tolerance, since Arabidopsis thaliana plants mutated the key enzyme responsible for synthesis (arginine decarboxilase, ADC; EC 4.1.1.19) are more sensitive than wild type Although it speculated over-expression of ADC genes may confer hampered by pleiotropic effects arising from...
There is a significant unmet need for clinical reflex tests that increase the specificity of prostate-specific antigen blood testing, longstanding but imperfect tool prostate cancer diagnosis. Towards this endpoint, we present results from discovery study identifies new markers in large-scale patient serum cohort using differentiating technologies deep proteomic interrogation. We detect known as well novel candidates. Through bioinformatic pathway enrichment and network analysis, reveal...
In recent years, there has been a growing interest in NADPH-oxidases which are involved the active generation of reactive oxygen species (ROS), owing to their role oxidative burst, signalling and damage derived from biotic abiotic stresses. NADPH oxidase activity is enhanced by some environmental cues, such as zinc deficiency chilling stress, where polyamines have suggested be modulation ROS generation. order further characterise oxidation during stress we exposed Lotus glaber Mill....
Salt stress has been frequently studied in its first osmotic phase. Very often, data regarding the second ionic phase is missing. It also suggested that Putrescine or/and Spermine could be responsible for salt resistance. In order to test this hypothesis under long-term stress, we obtained Arabidopsis thaliana transgenic plants harboring pRD29A::oatADC or pRD29A::GUS construction. Although was only polyamine significantly increased after acclimation lines, rendered no advantage kind of...
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Abstract Introduction: Our ~20,000 genes encode over ~1 million protein variants because of alternative splice forms, allelic variation and modification. Large-scale genomics studies the last decade have markedly increased our understanding cancer biology through analysis both tissue biofluids. However, similarly scaled deep unbiased proteomics has been impractical due to complex workflows required in We previously described Proteograph, a novel platform that leverages nano-bio interactions...