A5055317056- Neurobiology and Insect Physiology Research
- Circadian rhythm and melatonin
- Insect and Arachnid Ecology and Behavior
- Plant and animal studies
- Insect symbiosis and bacterial influences
- Sleep and Wakefulness Research
- Invertebrate Immune Response Mechanisms
- Plant Molecular Biology Research
- Animal Behavior and Reproduction
- Photoreceptor and optogenetics research
- Mosquito-borne diseases and control
- Plant and Biological Electrophysiology Studies
- Neuroscience and Neuropharmacology Research
- Species Distribution and Climate Change
- Diptera species taxonomy and behavior
- Dietary Effects on Health
- Plant Parasitism and Resistance
- Genetics, Aging, and Longevity in Model Organisms
- Physiological and biochemical adaptations
- Aquaculture disease management and microbiota
- Spaceflight effects on biology
- Insect Utilization and Effects
- Neuroscience of respiration and sleep
- Genetic diversity and population structure
- Neurological diseases and metabolism
Consejo Nacional de Investigaciones Científicas y Técnicas
2008-2024
University of Buenos Aires
2023-2024
Imperial College London
2015-2024
Centro Científico Tecnológico - San Juan
2024
Instituto de Neurología de Buenos Aires
2021-2022
Fundación Instituto Leloir
2008-2017
The recent development of automatised methods to score various behaviours on a large number animals provides biologists with an unprecedented set tools decipher these complex phenotypes. Analysing such data comes several challenges that are largely shared across acquisition platform and paradigms. Here, we present rethomics, R packages unifies the analysis behavioural datasets in efficient flexible manner. rethomics offers computational solution storing, manipulating visualising amounts...
Here, we present the use of ethoscopes, which are machines for high-throughput analysis behavior in Drosophila and other animals. Ethoscopes provide a software hardware solution that is reproducible easily scalable. They perform, real-time, tracking profiling by using supervised machine learning algorithm, able to deliver behaviorally triggered stimuli flies feedback-loop mode, highly customizable open source. can be built 3D printing technology rely on Raspberry Pi microcomputers Arduino...
In all animals, sleep pressure is under continuous tight regulation. It universally accepted that this regulation arises from a two-process model, integrating both circadian and homeostatic controller. Here we explore the role of environmental social signals as third, parallel controller homeostasis pressure. We show that, in Drosophila melanogaster males, after deprivation can be counteracted by raising their sexual arousal, either engaging flies with prolonged courtship activity or merely...
Sleepless fruit flies live, an unexpected finding that provides a new perspective on sleep for this and perhaps other species.
Rapid eye movement (REM) sleep has been hypothesized to promote emotional resilience, but any neuronal circuits mediating this have not identified. We find that in mice, somatostatin (Som) neurons the entopeduncular nucleus (EP
Living organisms use biological clocks to maintain their internal temporal order and anticipate daily environmental changes. In Drosophila, circadian regulation of locomotor behavior is controlled by ∼150 neurons; among them, neurons expressing the PIGMENT DISPERSING FACTOR (PDF) set period under free-running conditions. To date, it remains unclear how individual clusters integrate activity assemble a distinctive behavioral output. Here we show that BONE MORPHOGENETIC PROTEIN (BMP) signaling...
Circadian rhythms are conserved across kingdoms and coordinate physiology behavior for appropriate time-keeping. The neuronal populations that govern circadian described in many animal models, the current challenge is to understand how they interact control overt rhythms, remaining plastic enough respond adapt a changing environment. In Drosophila melanogaster, network comprises about 150 neurons, main synchronizer neuropeptide pigment-dispersing factor (PDF), released by well-characterized...
Drosophila is a well-established model to study the molecular basis of neurodegenerative diseases. We carried out misexpression screen identify genes involved in neurodegeneration examining locomotor behavior young and aged flies. hypothesized that progressive loss rhythmic activity could reveal novel mechanisms. One interesting candidates showing arrhythmicity has reduced enabled (ena) levels. ena down-regulation gave rise vacuolization specific regions adult brain. Abnormal staining...
Intertidal species have both circadian and circatidal clocks. Although the behavioral evidence for these oscillators is more than 5 decades old, virtually nothing known about their molecular clockwork. Pigment-dispersing hormones (PDHs) were originally described in crustaceans. Their insect homologs, pigment-dispersing factors (PDFs), a prominent role as clock output synchronizing signals released from neurons. We show that gene duplication crabs has led to two PDH genes (β-pdh-I β-pdh-II)....
The activation of the immune system upon infection exerts a huge energetic demand on an individual, likely decreasing available resources for other vital processes, like reproduction. factors that determine trade-off between defensive and reproductive traits remain poorly understood. Here, we exploit experimental tractability fruit fly Drosophila melanogaster to systematically assess impact pre-copulatory behaviour. Contrary expectations, found male flies undergoing continue display high...
Host behavioural changes are among the most apparent effects of infection. 'Sickness behaviour' can involve a variety symptoms, including anorexia, depression, and changed activity levels. Here, using real-time tracking profiling platform, we show that in Drosophila melanogaster, several systemic bacterial infections cause significant increases physical activity, extent this increase is predictor survival time some lethal infections. Using multiple bacteria D. melanogaster immune mutants,...
Animals adapt to the daily changes in their environmental conditions by means of genetically encoded circadian clocks. These clocks, found throughout tree life, regulate diverse biological functions, and allow periodical physiology behaviour. The molecular underpinnings these clocks have been extensively studied across taxa, revealing a brain-based system that coordinates rhythmic activities through neuronal networks signalling pathways. Entrainment, alignment internal rhythms with external...
Abstract In modern human societies, social isolation acts as a negative factor for health and life quality. On the other hand, interaction also has profound effects on animal human, impacting aggressiveness, feeding sleep, among many behaviors. Here, we observe that in fly Drosophila melanogaster these behavioral changes long-last even after ceased, suggesting socialization experience triggers plasticity. These modified behaviors maintain similar levels 24 h persist up to 72 h, although...
Abstract Animals have evolved neural circuits that allow them to generate adaptive behaviors their natural environment. Specific neuronal clusters depend on..... Circadian clocks organize the metabolism, physiology, and behavior of organisms throughout day–night cycle by controlling daily rhythms in gene expression at transcriptional post-transcriptional levels. While many transcription factors underlying circadian oscillations are known, splicing modulate these remain largely unexplored. A...
Abstract The recent development of automatised methods to score various behaviours on a large number animals provides biologists with an unprecedented set tools decipher these complex phenotypes. Analysing such data comes several challenges that are largely shared across acquisition platform and paradigms. Here, we present rethomics , R packages unifies the analysis behavioural datasets in efficient flexible manner. offers computational solution storing, manipulating visualising amounts...
Abstract Great efforts have been directed to the dissection of cell‐autonomous circadian oscillator in Drosophila . However, less information is available regarding how this controls rhythmic rest–activity cycles. We identified a viable allele roundabout , robo hy where period locomotor activity shortened. From its role axon‐pathfinding, we anticipated developmental defects clock‐relevant structures. produced minor architecture circuits essential for behaviour. ROBO's presence within circuit...
Abstract We present ethoscopes, machines for high-throughput analysis of behaviour in Drosophila and other animals. Ethoscopes provide a software hardware solution that is reproducible easily scalable; they perform, real-time, tracking profiling using supervised machine learning algorithm; can deliver behaviourally-triggered stimuli to flies feedback-loop mode; are highly customisable open source. be built 3D printing technology rely on Raspberry Pi microcomputers Arduino boards affordable...
Abstract Sleep appears to be a universally conserved phenomenon among the animal kingdom but whether this striking evolutionary conservation underlies basic vital function is still an open question. Using novel technologies, we conducted unprecedentedly detailed high-throughput analysis of sleep in fruit fly Drosophila melanogaster , coupled with life-long chronic and specific restriction. Our results show that some wild-type flies are virtually sleepless baseline conditions complete, forced...