A5086866155- Optical Imaging and Spectroscopy Techniques
- Photoacoustic and Ultrasonic Imaging
- Advanced Fluorescence Microscopy Techniques
- Photodynamic Therapy Research Studies
- Optical Coherence Tomography Applications
- Nanoplatforms for cancer theranostics
- Optical Polarization and Ellipsometry
- Surgical Simulation and Training
- Spectroscopy Techniques in Biomedical and Chemical Research
- Robotics and Sensor-Based Localization
- Infrared Thermography in Medicine
- Advanced Vision and Imaging
- Leaf Properties and Growth Measurement
- Augmented Reality Applications
- Thermoregulation and physiological responses
- Esophageal Cancer Research and Treatment
- Radiomics and Machine Learning in Medical Imaging
- Anatomy and Medical Technology
- Advanced Optical Sensing Technologies
- Spectroscopy and Chemometric Analyses
- Gold and Silver Nanoparticles Synthesis and Applications
- Ultrasound Imaging and Elastography
- Soft Robotics and Applications
- Non-Invasive Vital Sign Monitoring
- Organ and Tissue Transplantation Research
Imperial College London
2015-2024
University of Bristol
2024
St Mary's Hospital
2013-2020
St. Mary's Hospital
2013-2020
University College London
2017
Paul Hamlyn Foundation
2017
University of Leeds
2017
University of Liverpool
2014
NIHR Imperial Biomedical Research Centre
2007-2013
Imperial Valley College
2012
We demonstrate for the first time application of an endoscopic fluorescence lifetime imaging microscopy (FLIM) system to intraoperative diagnosis glioblastoma multiforme (GBM). The clinically compatible FLIM prototype integrates a gated (down 0.2 ns) intensifier with fiber-bundle (fiber image guide 0.5 mm diameter, 10,000 fibers gradient index lens objective NA, and 4 field view) provide access surgical field. Experiments conducted in three patients undergoing craniotomy tumor resection that...
Fluorescence lifetime imaging (FLIM) is a novel technique that generates image contrast between different states of tissue due to differences in fluorescence decay rates.To establish whether FLIM skin autofluorescence can provide useful basal cell carcinomas (BCCs) and surrounding uninvolved skin.Unstained excision biopsies 25 BCCs were imaged en face with following excitation 355 nm pulsed ultraviolet laser.Using we able distinguish areas BCC from an ex vivo study. Significant reductions...
Polarization imaging has been recognized as a potentially powerful technique for probing the microstructural information and optical properties of complex biological specimens.Recently, we have reported Mueller matrix microscope by adding polarization state generator analyzer (PSG PSA) to commercial transmission-light microscope, applied it differentiate human liver cervical cancerous tissues with fibrosis.In this paper, apply quantitative detection breast ductal carcinoma samples at...
Intra-operative imaging techniques for obtaining the shape and morphology of soft-tissue surfaces in vivo are a key enabling technology advanced surgical systems. Different optical 3-D surface reconstruction laparoscopy have been proposed, however, so far no quantitative comparative validation has performed. Furthermore, robustness methods to clinically important factors like smoke or bleeding not yet assessed. To address these issues, we formed joint international initiative with aim...
Abstract The contrast mechanism of medical endoscopy is mainly based on metrics optical intensity and wavelength. As another fundamental property light, polarization can not only reveal tissue scattering absorption information from a different perspective, but also provide insight into directional birefringence properties to monitor pathological changes in collagen elastin. Here we demonstrate low cost wide field high definition Mueller polarimetric endoscope with minimal alterations rigid...
Because of the critical role large neutral amino acid transporter-1 (LAT-1) in promoting tumor growth and proliferation, it is fast emerging as a highly attractive biomarker for imaging treatment human malignancies, including breast cancer. While multibranched gold nanoparticles (AuNPs) have emerged promising modality photothermal therapy (PTT) cancers, some key challenges limiting their clinical translation lie need to develop reproducible cost-effective synthetic methods well selective...
Advances in vectorial polarization-resolved imaging are bringing new capabilities to applications ranging from fundamental physics through clinical diagnosis. Imaging polarimetry requires determination of the Mueller matrix (MM) at every point, providing a complete description an object's properties. Despite forming comprehensive representation, MM does not usually provide easily interpretable information about internal structure. Certain simpler metrics derived subsets elements. These...
The standard-of-care for the detection of laryngeal pathologies involves distinguishing suspicious lesions from surrounding healthy tissue via contrasts in colour and texture captured by white-light endoscopy. However, technique is insufficiently sensitive thus leads to unsatisfactory rates false negatives. Here we show that can be better detected real time taking advantage differences light-polarization properties cancer tissues. By measuring polarized-light retardance depolarization,...
A compact clinically compatible fluorescence lifetime imaging microscopy (FLIM) system was designed and built for intraoperative disease diagnosis validated in vivo a hamster oral carcinogenesis model. This apparatus allows the remote image collection via flexible probe consisting of gradient index objective lens fiber bundle. Tissue autofluorescence (337 nm excitation) imaged using an intensified CCD with gate width down to 0.2 ns. We demonstrate significant contrast between tumor...
Optical imaging of tissue autofluorescence has the potential to provide rapid label-free screening and detection surface tumors for clinical applications, including when combined with endoscopy. Quantitative intensity-based contrast is notoriously difficult spectrally resolved does not always sufficient contrast. We demonstrate that fluorescence lifetime (FLIM) applied intrinsic can directly a range tumors, in gastrointestinal tissues, using compact, clinically deployable instrumentation...
Abstract A clinically compatible fluorescence lifetime imaging microscopy (FLIM) system was developed. The applied to intraoperative in vivo of head and neck squamous cell carcinoma (HNSCC). endoscopic FLIM prototype integrates a gated (down 0.2 ns) intensifier fiber-bundle endoscope (0.5-mm-diameter, 10,000 fibers with gradient index lens objective 0.5 NA, 4-mm field view), which provides access the surgical field. Tissue autofluorescence induced by pulsed laser (337 nm, 700 ps pulse width)...
The application of time-resolved fluorescence spectroscopy (TRFS) to in vivo tissue diagnosis requires a method for fast acquisition decay profiles multiple spectral bands. This study focusses on development clinically compatible fiber-optic based multispectral TRFS (ms-TRFS) system together with validation its accuracy and precision lifetime measurements. It also presents the expansion this technique into an imaging method. A tandem array dichroic beamsplitters filters was used record at...
Tissue polarimetric imaging measures Mueller matrices of tissues or Stokes vectors the emergent light from (normally using incidence with a fixed polarization state) over field view, and has demonstrated utility in number surgical diagnostic applications.Here we introduce compact complete imager that can work for multiple wavelength bands frame-rate suitable real-time applications.The was validated standard polarizing components, then employed as state analyzer polarimeter standalone...
Mueller matrix polarimetric imaging has shown potential in tissue diagnosis but is challenging to implement endoscopically. In this work, a narrow band 3 × endoscope was designed by rotating the generate 0°, 45° and 90° linearly polarized illumination positioning filter wheel front of camera containing three polarisers permit polarization state analysis for backscattered light. The system validated with linear polarizer diffuse reflection target. Initial measurements matrices on rat are...
Intraoperative monitoring of tissue oxygen saturation (StO2 ) has potentially important applications in procedures such as organ transplantation or colorectal surgery, where successful reperfusion affects the viability and integrity repaired tissues. In this paper a liquid crystal tuneable filter-based multispectral imaging (MSI) laparoscope is described. Motion-induced image misalignments are reduced, using feature-based registration, before regression reflectance spectra to calculate...
BACKGROUND : Artificial intelligence (AI) research in colonoscopy is progressing rapidly but widespread clinical implementation not yet a reality. We aimed to identify the top priorities. METHODS An established modified Delphi approach for priority setting was used. Fifteen international experts, including endoscopists and translational computer scientists/engineers, from nine countries participated an online survey over 9 months. Questions related AI were generated as long-list first round,...