A5101768016- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 detection and testing
- Respiratory viral infections research
- Polyomavirus and related diseases
- Metabolomics and Mass Spectrometry Studies
- Herpesvirus Infections and Treatments
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Advanced Biosensing Techniques and Applications
- Parvovirus B19 Infection Studies
- Toxoplasma gondii Research Studies
- Traditional Chinese Medicine Analysis
- Atherosclerosis and Cardiovascular Diseases
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- Bacterial Identification and Susceptibility Testing
- RNA modifications and cancer
- Cytomegalovirus and herpesvirus research
- Eicosanoids and Hypertension Pharmacology
- Intensive Care Unit Cognitive Disorders
- Pharmacological Effects of Natural Compounds
- Rabies epidemiology and control
Shanghai Public Health Clinical Center
2021-2025
Yangzhou University
2024-2025
Union Hospital
2020-2024
Jilin University
2019-2024
Union Hospital
2019-2024
Shanghai Clinical Research Center
2021-2022
Huazhong Agricultural University
2021
Second Affiliated Hospital of Dalian Medical University
2021
Dalian Medical University
2021
First Hospital of Jilin University
2020
Viral evolution impacts diagnostic test performance through the emergence of variants with sequences affecting efficiency primer binding. Such that evade detection by nucleic acid-based tests are subject to selective pressure, enabling them spread more efficiently. Here, we report a variant-tolerant for SARS-CoV-2 using loop-mediated isothermal amplification (LAMP) assay containing high-fidelity DNA polymerase and polymerase-medicated probe (HFman probe). In addition demonstrating high...
ABSTRACT Staphylococcus aureus ( S. ) is a leading cause of bacteremia and blood stream infections. Methicillin-resistant (MRSA) that first appeared in 1961 often caused hospital-acquired infections (HAIs) community-acquired (CAIs) was associated with high mortality rate. Accurate rapid point-of-care testing (POCT) MRSA crucial for clinical management treatment infections, as well the prevention control HAIs CAIs. Here, we reported novel extraction-free dual HiFi-LAMP assay discriminative...
Long non-coding RNA maternally expressed gene 3 (MEG3) is related to the occurrence and development of non-small cell lung cancer (NSCLC). However, function underlying molecular mechanisms MEG3 in stem cells (LCSCs) are still unclear. LCSCs were determined using fluorescence-activated sorting (FACS). qRT-PCR western blot performed examine expressions MEG3, miR-650, solute carrier family 34 member 2 (SLC34A2), octamer-binding transcription factor 4 (Oct4), CD133. Sphere assay was employed...
Highlights•A HiFi-LAMP assay for M. leprea has a LOD of 43 copies/25 μL reaction•A higher detection rate leprae in nasal swabs than oral swabs•The sensitivity the qPCR assay•The extraction-free short sample-to-answer time (<30 mins)AbstractBackgroundTimely and accurate Mycobacterium (M. leprae) is crucial efficient treatment early intervention leprosy, which requires simple rapid assay.MethodsAn was developed leprae. The performance assessed by comparing with nested PCR assays using clinical...
Hantaviruses are zoonotic pathogens that widely distributed worldwide. Hantaan virus (HTNV) and Seoul (SEOV) two most common hantaviruses infect humans cause hemorrhagic fever with renal syndrome (HFRS). Rapid sensitive detection of HTNV SEOV crucial for surveillance, clinical treatment management HFRS. This study aimed to develop a rapid HFman probe-based mulstiplex reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay simultaneously detect SEOV. A novel multiplex...
Human immunodeficiency virus (HIV) rapid variant mutations challenges diagnostic assays. Our variant-tolerant isothermal nucleic acid based amplification technique enables reliable detection at the point-of-care.
Background BK virus (BKV)-associated nephropathy (BKVN) is one of the leading causes renal dysfunction and graft loss in transplant recipients. Early monitoring BKV urine crucial to minimize deleterious effects caused by this on preservation function. Methods We report a simple, rapid, sensitive loop-mediated isothermal amplification (LAMP) assay using an HFman probe for detecting urine. To evaluate performance assay, comparison probe-based LAMP (HF-LAMP) with two qPCR assays was performed...
Since China abandoned the zero-COVID policy at end of 2022, a wave severe Flu pandemic emerged in China. Rapid and accurate diagnosis Influenza A virus (IAV) is critical for clinical management therapeutic decision-making patients with fever. Here, we reported novel IAV HF-LAMP assay, which can be performed purified RNA or directly using samples. The assays samples have high sensitivity limit detection (LOD) 9.6 copies/reaction, 9900 copies/mL, short sample-to-answer times 36 50 min,...
Abstract Background This study aimed to identify potential circular ribonucleic acid (circRNA) signatures involved in the pathogenesis of early-stage lung adenocarcinoma (LAC). Methods The circRNA sequencing dataset LAC was downloaded from Gene Expression Omnibus database. First, differentially expressed circRNAs (DEcircRNAs) between tumour and non-tumour tissues were screened. Then, corresponding miRNAs their target genes predicted. In addition, prognosis-related identified using survival...
Toxoplasmosis caused by Toxoplasma gondii is a serious disease threatening human and animal health. People can be infected with T. ingesting raw pork contaminated cysts or oocysts. Serological test sensitive specific method usually used for large-scale diagnosis of infection in humans animals (such as pigs). Commercial pig antibody ELISA diagnostic kits are expensive, which limits their use; moreover, the wide antigen composition these still unclear difficult to standardize. The multiepitope...
Introduction High metabolic plasticity, as one of the hallmarks multiple cancers, usually promotes cancer growth and metastasis. Therefore, enzymes could be selective targets in suppressing invasion cells. It is generally believed that hexokinase 2 (HK2) catalysing glucose into glucose-6-phosphate first step metabolism. unclear, however, if HK2 involved lung (LC) proliferation Material methods Tumours adjacent tissue from LC patients were used to study expression by RT-PCR western blot...
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