A5080887613- interferon and immune responses
- HIV Research and Treatment
- RNA Research and Splicing
- Viral Infections and Immunology Research
- RNA regulation and disease
- HIV/AIDS drug development and treatment
- SARS-CoV-2 and COVID-19 Research
- Cytomegalovirus and herpesvirus research
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Hepatitis C virus research
- CRISPR and Genetic Engineering
- Viral-associated cancers and disorders
- Virus-based gene therapy research
- Proteoglycans and glycosaminoglycans research
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- Fibroblast Growth Factor Research
- Immune Cell Function and Interaction
- Herpesvirus Infections and Treatments
- Mosquito-borne diseases and control
- Immunotherapy and Immune Responses
- Parvovirus B19 Infection Studies
- Cancer Research and Treatments
- Biochemical and Molecular Research
Institute of Biophysics
2012-2024
University of Chinese Academy of Sciences
2016-2024
Chinese Academy of Sciences
2012-2023
Czech Academy of Sciences, Institute of Biophysics
2022
Columbia University
1994-2008
Moffitt Cancer Center
2000-2006
Institute of Microbiology
2002-2006
Tufts University
2006
Tsinghua University
2005
Howard Hughes Medical Institute
1997-2005
Fibroblast growth factors (FGFs) are essential molecules for mammalian development. The nine known FGF ligands and the four signaling receptors (and their alternatively spliced variants) expressed in specific spatial temporal patterns. activity of this pathway is regulated by ligand binding specificity, heparan sulfate proteoglycans, differential capacity individual receptors. To determine potentially relevant ligand-receptor pairs we have engineered mitogenically responsive cell lines...
The genus Coronavirus contains about 25 species of coronaviruses (CoVs), which are important pathogens causing highly prevalent diseases and often severe or fatal in humans animals. No licensed specific drugs available to prevent their infection. Different host receptors for cellular entry, poorly conserved structural proteins (antigens), the high mutation recombination rates CoVs pose a significant problem development wide-spectrum anti-CoV vaccines. CoV main proteases (M(pro)s), key...
Cells have evolved multiple mechanisms to inhibit viral replication. To identify previously unknown antiviral activities, we screened mammalian complementary DNA (cDNA) libraries for genes that prevent infection by a genetically marked retrovirus. Virus-resistant cells were selected from pools of transduced clones, and an active cDNA was recovered. The gene encodes CCCH-type zinc finger protein designated ZAP. Expression the caused profound specific loss messenger RNAs (mRNAs) cytoplasm...
ABSTRACT The rat zinc-finger antiviral protein (ZAP) was recently identified as a host conferring resistance to retroviral infection. We analyzed ZAP's ability inhibit viruses from other families and found that ZAP potently inhibits the replication of multiple members Alphavirus genus within Togaviridae , including Sindbis virus, Semliki Forest Ross River Venezuelan equine encephalitis virus. However, expression did not induce broad-spectrum state some viruses, vesicular stomatitis...
The zinc-finger antiviral protein (ZAP) was originally identified as a host factor that inhibits the replication of Moloney murine leukemia virus. Here we report ZAP HIV-1 infection by promoting degradation specific viral mRNAs. Overexpression rendered cells resistant to in expression level-dependent manner, whereas depletion endogenous enhanced infection. Both human and rat inhibited propagation replication-competent HIV-1. specifically targeted multiply spliced but not unspliced or singly...
Abstract Liver cancer stem cells (CSCs) may contribute to the high rate of recurrence and heterogeneity hepatocellular carcinoma (HCC). However, biology hepatic CSCs remains largely undefined. Through analysis transcriptome microarray data, we identify a long noncoding RNA (lncRNA) called lncBRM , which is highly expressed in liver HCC tumours. LncBRM required for self-renewal maintenance tumour initiation. In CSCs, associates with BRM initiate BRG1/BRM switch BRG1-embedded BAF complex...
Zinc-finger antiviral protein (ZAP) is a host factor that specifically inhibits the replication of Moloney murine leukemia virus (MLV) and Sindbis (SIN) by preventing accumulation viral mRNA in cytoplasm. In previous studies, we demonstrated ZAP directly binds to its specific target mRNAs. this article, provide evidence indicating recruits RNA processing exosome degrade RNA. comigrated with sucrose or glycerol velocity gradient centrifugation. Immunoprecipitation coprecipitated components....
ABSTRACT The zinc finger antiviral protein (ZAP) is a recently isolated host factor. It specifically inhibits the replication of Moloney murine leukemia virus (MLV) and Sindbis (SIN) by preventing accumulation viral RNA in cytoplasm. For this report, we mapped sequences that are sensitive to ZAP inhibition. were cloned into luciferase reporter analyzed for ability mediate ZAP-dependent destabilization reporter. sequence MLV was 3′ long terminal repeat; SIN multiple fragments. fragment...
Fibroblast growth factor (FGF) receptors (FGFRs) are structurally related receptor protein tyrosine kinases encoded by four distinct genes. Activation of FGFR-1, -2, and -3 FGFs induces mitogenic responses in various cell types, but the potential FGFR-4 has not been previously explored. We have compared properties BaF3 murine lymphoid cells L6 rat myoblast engineered to express FGFR-1 or FGFR-4. Acidic FGF binds with high affinity elicits phosphorylation displayed on cells, only activation...
ABSTRACT Zinc finger antiviral protein (ZAP) is a host factor that specifically inhibits the replication of certain viruses by binding to viral mRNAs and repressing translation and/or promoting degradation target mRNA. In addition, ZAP regulates expression cellular genes. Here, we report tripartite motif-containing 25 (TRIM25), ubiquitin E3 ligase, required for activity ZAP. Downregulation endogenous TRIM25 abolished ZAP's activity. The ligase this regulation. mediated ubiquitination, but...
Zinc-finger antiviral protein (ZAP) is a host factor that specifically restricts wide range of viruses. ZAP selectively binds to CG-dinucleotide-enriched RNA sequences and recruits multiple degradation machines degrade target viral RNA. However, the molecular mechanism structural basis for recognition specific are not clear. Here, we report crystal structure N-terminal domain bound CG-rich single-stranded RNA, providing its CG dinucleotide additional guanine cytosine. The four zinc fingers...
Zinc finger antiviral protein (ZAP) is a host factor that specifically inhibits the replication of certain viruses. There are two ZAP isoforms arising from alternative splicing, which differ only at C termini. It was recently reported long isoform (ZAPL) promotes proteasomal degradation influenza A virus (IAV) proteins PA and PB2 through C-terminal poly(ADP-ribose) polymerase (PARP) domain, missing in short form (ZAPS), this activity antagonized by viral PB1. Here, we report IAV expression...
The traditional classification of nucleic acid polymerases as either DNA or RNA is based, in large part, on their fundamental preference for the incorporation deoxyribonucleotides ribonucleotides during chain elongation. refined structure determination Moloney murine leukemia virus reverse transcriptase, a strict polymerase, recently allowed prediction that single amino residue at active site might be responsible discrimination against 2′OH group an incoming ribonucleotide. Mutation this...
The zinc-finger antiviral protein (ZAP) specifically inhibits the replication of many viruses by preventing accumulation viral mRNAs in cytoplasm. ZAP directly binds to and recruits RNA exosome degrade target RNA. In present study, we identified p72 DEAD box helicase, but not highly similar helicase p68, as a ZAP-interacting protein. binding domain was mapped its N-terminal portion, whereas both N- C-terminal domains bound ZAP. Overexpression reduced ZAP's activity, overexpression...
The zinc-finger antiviral protein (ZAP) is a host factor that inhibits the replication of many viruses by preventing accumulation viral mRNAs in cytoplasm. ZAP specifically binds to mRNA and recruits cellular RNA degradation machinery degrade target RNA. In this article, we will review work date understanding mechanisms which promotes decay discuss future research directions further investigate function underlying ZAP.
Tristetraprolin (TTP) regulates the expression of AU-rich element-containing mRNAs through promoting degradation and repressing translation target mRNA. While mechanism for mRNA has been extensively studied, underlying translational repression is not well established. Here, we show that TTP recruits eukaryotic initiation factor 4E2 (eIF4E2) to repress translation. interacted with eIF4E2 but eIF4E. Overexpression enhanced TTP-mediated repression, downregulation endogenous or overexpression a...