A5102020212- 3D Printing in Biomedical Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Pancreatic function and diabetes
- Electrohydrodynamics and Fluid Dynamics
- Liver physiology and pathology
- Pluripotent Stem Cells Research
- Microfluidic and Bio-sensing Technologies
- Diet and metabolism studies
- Neuroscience and Neural Engineering
- Cancer-related molecular mechanisms research
- Tissue Engineering and Regenerative Medicine
- Diabetes and associated disorders
- Additive Manufacturing and 3D Printing Technologies
- Ubiquitin and proteasome pathways
- Cancer-related Molecular Pathways
- Gastrointestinal Tumor Research and Treatment
- Cancer Cells and Metastasis
- ATP Synthase and ATPases Research
- Medical Research and Treatments
- Single-cell and spatial transcriptomics
- Computational Drug Discovery Methods
- Regional Development and Environment
- Pharmacological Effects and Toxicity Studies
- MicroRNA in disease regulation
- Metabolism, Diabetes, and Cancer
Dalian Institute of Chemical Physics
2017-2025
Chinese Academy of Sciences
2017-2025
Taixing People's Hospital
2024
Zhejiang University
2018-2022
First Affiliated Hospital Zhejiang University
2022
University of Chinese Academy of Sciences
2020-2021
Sir Run Run Shaw Hospital
2018
Dalian University
2017-2018
Anhui Medical University
2016
Jiangyin People's Hospital
2014
Liver organoids derived from human pluripotent stem cells (PSCs) represent a new type of in vitro liver model for understanding organ development, disease mechanism and drug testing. However, engineering with favorable functions controlled cellular microenvironment remains challenging. In this work, we present strategy induced PSCs (hiPSCs) 3D perfusable chip system by combining cell biology microengineering technology. This approach enabled formation hiPSC-based embryoid bodies (EBs), situ...
A biomimetic glomerulus-on-a-chip microdevice was created to recapitulate a disease model of diabetic nephropathy.
Organoids derived from self-organizing stem cells represent a major technological breakthrough with the potential to revolutionize biomedical research. However, building high-fidelity organoids in reproducible and high-throughput manner remains challenging. Here, droplet microfluidic system is developed for controllable fabrication of hybrid hydrogel capsules, which allows massive 3D culture formation functional uniform islet human-induced pluripotent (hiPSCs). In this all-in-water system,...
Abstract Type 2 diabetes mellitus (T2DM) is a systematic multi‐organ metabolic disease, which characterized by the dynamic interplay among different organs. The increasing incidence of T2DM reflects an urgent need for development in vitro human‐relevant models disease study and drug therapy. Here, new microfluidic multi‐organoid system developed that recapitulates human liver‐pancreatic islet axis normal states. contains two compartmentalized regions connected microchannel network, enabling...
The poor predictive power of existing preclinical models has spurred efforts to develop human-relevant for accurate assessment drug safety. In this work, we developed a multi-organoids-on-a-chip system derived from human induced pluripotent stem cells (hiPSCs), which allows the cardiac safety an antidepressant drug, following liver metabolism in vitro. This liver-heart organoids-on-chip device contains compartmentalized chambers separated by porous membrane, permits co-culture 3D organoids...
Nonalcoholic fatty liver disease (NAFLD) is a common metabolic and progressive disease, which has emerged as major cause of chronic worldwide. It characterized by the process ranging from simple steatosis to nonalcoholic steatohepatitis. However, deep understanding NAFLD progression remains challenging due lack proper in vitro human models. In this work, we proposed new strategy establish model based on human-induced pluripotent stem cell (hiPSC)-derived organoids-on-a-chip system. This...
Metabolic syndrome (MetS) is characterized by a cluster of metabolic disorders including obesity, dyslipidemia, hyperglycemia, and hypertension. Here, we report that 27 microRNAs were found to be expressed differently in serum urine samples MetS patients compared control subjects on microarray analysis. Further qualitative real time- polymerase chain reaction analyses confirmed circulating levels miR-143-3p significantly elevated with controls, both samples. After accounting for confounding...
Prenatal exposure to environmental insults can increase the risk of developing neurodevelopmental disorders. Administration antiepileptic drug valproic acid (VPA) during pregnancy is tightly associated with a high neurological disorders in offspring. However, lack an ideal human model hinders our comprehensive understanding impact VPA on fetal brain development, especially early gestation. Herein, we present first report indicating effects development at stages using engineered cortical...
A flexible microfluidic strategy to generate grooved microfibers relies on <italic>in situ</italic> gelling of different concentrations alginate as anisotropic scaffolds for guiding cell alignment.
The cell encapsulation technology is promising for generation of functional carriers with well-tailored structures efficient transplantation and immunoprotection cells/tissues. Stem organoids are highly potential recapitulating the intricate architectures functionalities native organs also providing an unlimited source cellular replacement therapy. However, it remains challenging loading hundreds micrometers size by current existing carriers. Herein, a simple facile coextrusion strategy...
Abstract Porous GelMA microgels are promising scaffolds for biomedical research and regenerative medicine due to their special topology, cell‐loading capacity, high biocompatibility. However, the traditional preparation of porous is often limited by some complex postprocessing processes such as freeze‐drying swelling equilibrium. Herein, we present a simple strategy in situ generation cell carriers integrating placeholder polyethylene oxide (PEO) chaotic advection effect droplets...
Abstract Objective To investigate the predictive value of blood urea nitrogen to serum albumin ratio for in-hospital and out-of-hospital mortality in critically ill patients with diabetic ketoacidosis. Methods Data were obtained from Medical Information Mart Intensive Care III (MIMIC III) database, all eligible participants categorized into two groups based on BAR cutoff value. Multiple logistic regression analysis was conducted determine association between mortality. The Kaplan–Meier (K–M)...
Microgels are advanced scaffolds for tissue engineering due to their proper biodegradability, good biocompatibility, and high specific surface area effective oxygen nutrient transfer. However, most of the current monodispersed microgel fabrication systems rely heavily on various precision pumps, which highly increase cost complexity downstream application. In this work, we developed a simple facile system controllable generation uniform alginate microgels by integrating gas-shearing strategy...
C23 is an abundant and multi-functional protein, which plays important role in various biological processes, including ribosome biogenesis maturation, cell cycle checkpoints transcriptional regulation [1, 2]. However, the of controlling tumorigenesis has not been well defined. Here we report that highly expressed cancer cells elevated expression facilitates proliferation vitro tumor xenograft growth vivo. Notably, binds to p53 through its GAR domain suppresses activity under DNA damage...
Microengineered Multi-Organoid System In article number 2103495 by Jianhua Qin and co-workers, a novel microfluidic multi-organoid system derived from hiPSCs is proposed to recapitulate human liver-pancreatic islet axis under both physiological pathological conditions. It allows investigate the dynamic interplay between liver in type 2 diabetes, providing unique platform for study of complex pathogenesis metabolic diseases development new therapy.
<p indent="0mm">Organoids-on-a-chip is a newly emerging and frontier field with marriage of organoids microfluidic organ-on-a-chip technology. The synergistic engineering strategies can be adopted to build organ model systems in high fidelity, hold great potential studies development, disease modeling, drug discovery regenerative medicine. In this review, we introduce the origin, key features recent progress organoids-on-chips. future opportunities major challenges advance...
Objective To explore the protective effect and probable mechanism of JNK inhibitor SP600125 on hippocampal neurons in rats with status epilepsy following lithium-pilocarpine. Methods 48 Wistar rats, accordance random number table, were divided into control, epilepticus (SE) group (SP). HE staining fluorescent TUNEL method used to observe pathological changes neuronal apoptosis area each group. Western blot was applied detect phosphorylation expression its downstream effector molecule...
Objective: Calcific aortic valve disease (CAVD) is a prevalent type of valvular heart disease, its association with dyslipidemia remains controversial. Methods: Of 449 CAVD patients who underwent replacement, 228 formed the calcification (AVC) group, and 221 were non-calcification group. We retrospectively reviewed preoperative one-year postoperative plasma lipoprotein levels both performed logistic regression to evaluate factors associated AVC. Results: Preoperatively, AVC had significantly...
Abstract Background Immunoinflammatory and nutritional markers such as peripheral blood neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte (PLR) onodera’s prognostic index (OPNI) have gained considerable attention revealed preliminaryly in gastrointestinal stromal tumor (GIST). Methods In this study, we firstly investigated the value of OPNI GIST treated with or without TKIs based on propensity score matching (PSM) method. All patients had received surgical resection for primary...