A5101680922- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- SARS-CoV-2 detection and testing
- Viral gastroenteritis research and epidemiology
- COVID-19 Clinical Research Studies
- Mosquito-borne diseases and control
- Dental Research and COVID-19
- Computational Drug Discovery Methods
- Infection Control and Ventilation
- Neonatal Respiratory Health Research
- Insect symbiosis and bacterial influences
- COVID-19 epidemiological studies
- Blood groups and transfusion
- vaccines and immunoinformatics approaches
- Virus-based gene therapy research
- Hepatitis Viruses Studies and Epidemiology
- Diverse Scientific Research Studies
- Long-Term Effects of COVID-19
- Adipokines, Inflammation, and Metabolic Diseases
- Plant-based Medicinal Research
- Neonatal and Maternal Infections
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Plant tissue culture and regeneration
- Chemical synthesis and alkaloids
Lanzhou Veterinary Research Institute
2024
Chinese Academy of Agricultural Sciences
2024
Lanzhou University
2024
Gansu Provincial Center for Disease Control and Prevention
2024
Southwest University
2024
Chinese Academy of Sciences
2020-2023
Wuhan Institute of Virology
2020-2022
University of Chinese Academy of Sciences
2021
Northwest A&F University
2014
Sun Yat-sen University
2008-2012
The emerging SARS-CoV-2 infection associated with the outbreak of viral pneumonia in China is ongoing worldwide. There are no approved antiviral therapies to treat this disease. Here we examined abilities three broad-spectrum compounds gemcitabine, lycorine and oxysophoridine against cell culture. We found that all tested inhibited replication Vero-E6 cells at noncytotoxic concentrations. effect gemcitabine was suppressed efficiently by cytidine nucleosides. Additionally, combination had an...
Abstract The coronavirus disease 2019 (COVID-19) pandemic is caused by infection with the severe acute respiratory syndrome 2 (SARS-CoV-2), which spread primary via droplets and infects lungs. Currently widely used cell lines animals are unable to accurately mimic human physiological conditions because of abnormal status (transformed or cancer cells) species differences between humans. Organoids stem cell-derived self-organized three-dimensional culture in vitro model natural organs. Here we...
Wastewater-based epidemiology (WBE) has emerged as an effective environmental surveillance tool in monitoring fecal-oral pathogen infections within a community. Congruently, SARS-CoV-2, the etiologic agent of COVID-19, been demonstrated to infect gastrointestinal tissues, and be shed feces. In present study, SARS-CoV-2 RNA was concentrated from wastewater, sludge, surface water, ground sediment, soil samples municipal hospital wastewater systems related environments Wuhan during COVID-19...
We utilized archived data from the Five-hundred-meter Aperture Spherical Radio Telescope (FAST) to analyze single-pulse profile morphology of PSR J1935$+$1616 (B1933$+$16). The results show that exhibits significant micropulses as well various changes in morphology. In FAST data, a total 969 single pulses with microstructure were identified, accounting for 9.69$\%$ pulse sample, characteristic widths $127.63^{+70.74}_{-46.25}$ $\mu$s. About half these display quasiperiodic micropulses,...
BackgroundThe recent COVID-19 pandemic highlights the need for efficacious virucidal products to limit spread of SARS-CoV-2. Several studies have suggested that alcohol-based sanitizers and some disinfectants are effective. While activity data low-level lacking conclusions not clear yet.MethodsWe evaluated 2 quaternary ammonium compounds (QAC) (MICRO-CHEM PLUS FWD), W30 (an amphoteric surfactant), Medical EtOH against Suspension tests covering different concentration contact time were...
The global coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome 2 (SARS-CoV-2) has become greatest worldwide public health threat of this century, which may predispose multi-organ failure (especially lung) and death despite numerous mild moderate symptoms. Recent studies have unraveled molecular clinical characteristics infectivity, pathogenicity, immune evasion SARS-CoV-2 thus improved development many different therapeutic strategies to combat...
Non-structural protein 2 (NSP2) of PRRSV is highly variable and plays crucial roles in the virus’s life cycle. To elucidate function NSP2 during infection, we identified SH3KBP1 as an NSP2-interacting host using mass spectrometry. Exogenous expression significantly inhibited replication by enhancing IFN-I related ISGs production. Conversely, knockdown promoted viral downregulating levels. In vivo experiments revealed that Sh3kbp1 -/- mice were more susceptible to VSV exhibiting reduced serum...
Objective: Mosquitoes are medically important arthropod vectors that harbor a variety of viruses. Geography and climate known to be associated with variations in mosquito density, species viromes. Our study investigated the dynamic changes populations, compositions viromes regularly disinfected environment Wuhan, China, during 2020. Methods: Traps were set different habitats, including an urban residential area, two hospitals, scenic area pig farm rural region between April October The...
Ebola virus is among the most dangerous viral pathogens, with a case fatality rate of up to 90%. Since 2013, two largest and complex outbreaks in West Africa have revealed lack investigation on this notorious virus.
Abstract Modern human activity is profoundly changing our relationship with microorganisms the startling rise in rate of emerging infectious diseases. Nipah virus together Ebola and SARS-CoV-2 are prominent examples. Since COVID-19 West African disease outbreak, different chemical disinfectants have been developed for preventing direct spread viruses their efficacy has also evaluated. However, there currently no published studies disinfection virus. In this study, virucidal three (Micro-Chem...
Abstract The coronavirus disease 2019 (COVID-19) pandemic is caused by infection with the severe acute respiratory syndrome 2 (SARS-CoV-2), which spread primary via droplets and infects lungs. Currently widely used cell lines animals are unable to accurately mimic human physiological conditions because of abnormal status (transformed or cancer cells) species differences between humans. Organoids stem cell-derived self-organized three-dimensional culture in vitro model natural organs. Here we...
Abstract Wastewater-based epidemiology (WBE) has emerged as an effective environmental surveillance tool in monitoring fecal-oral pathogen infections within a community. Congruently, SARS-CoV-2 virus, the etiologic agent of COVID-19, been demonstrated to infect gastrointestinal tissues, and be shed feces. In present study, RNA was concentrated from wastewater, sludge, surface water, ground soil samples municipal hospital wastewater systems related environment Wuhan during COVID-19 middle low...
<title>Abstract</title> Asparagine synthetase AS-B plays a pivotal role in the response to tobacco mosaic virus (TMV) infection. In this study, evolutionarily conserved NbAS-B was found induce both antiviral and growth-promoting responses an expression intensity-dependent manner Nicotiana benthamiana. These effects were mediated through glutamate-dependent enhancement of salicylic acid (SA) signaling reprogramming photosynthesis pathway. Further, based on stronger resistance induction...
The purpose of this study was to determine the proper concentration wortmannin that effectively inhibits PI3K/AKT but does not affect proliferation and apoptosis primary porcine preadipocytes. Firstly, preadipocytes were isolated their abilities be induced differentiation into mature adipocytes evaluated. then treated with different concentrations wortmannin, cells detected methanethiosulfonate (MTS). Annexin V- FITC/PI double-staining used detect level cell apoptosis. apoptosis-related gene...
Given that ebolavirus causes severe and frequently lethal disease, its rapid accurate detection using available validated methods is essential for controlling infection. Real-time reverse-transcription PCR (RT-PCR) has proven to be an invaluable tool ebolaviruses diagnostics. Many assays with different targets have been developed, but they not externally compared or validated, limits of are uniformly reported. Here we evaluated the sensitivity, reproducibility specificity 23 in-house under...