A5101864009- Hepatitis B Virus Studies
- SARS-CoV-2 and COVID-19 Research
- Poxvirus research and outbreaks
- Bacillus and Francisella bacterial research
- Neonatal Respiratory Health Research
- COVID-19 Clinical Research Studies
- Intracranial Aneurysms: Treatment and Complications
- Viral gastroenteritis research and epidemiology
- Intracerebral and Subarachnoid Hemorrhage Research
- Marine Sponges and Natural Products
- Marine Invertebrate Physiology and Ecology
- Autophagy in Disease and Therapy
- 3D Shape Modeling and Analysis
- Acute Ischemic Stroke Management
- Mast cells and histamine
- Venomous Animal Envenomation and Studies
- Hepatitis Viruses Studies and Epidemiology
- Liver Disease Diagnosis and Treatment
- Protein Structure and Dynamics
- Calcium signaling and nucleotide metabolism
- Computer Graphics and Visualization Techniques
- Virology and Viral Diseases
- RNA and protein synthesis mechanisms
- Synthetic Aperture Radar (SAR) Applications and Techniques
- Seaweed-derived Bioactive Compounds
Wuhan Institute of Virology
2020-2025
Chinese Academy of Sciences
2020-2025
University of Chinese Academy of Sciences
2022-2025
Yantai University
2024-2025
Institute of Zoology
2021
Sichuan University
2021
Tianjin Normal University
2006
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused the ongoing disease 2019 pandemic. How SARS-CoV-2 regulates cellular responses to escape clearance by host cells is unknown. Autophagy an intracellular lysosomal degradation pathway for of various cargoes, including viruses. Here, we systematically screened 28 viral proteins and identified that ORF3a strongly inhibited autophagic flux blocking fusion autophagosomes with lysosomes. colocalized lysosomes interacted VPS39,...
Abstract The coronavirus disease 2019 (COVID-19) pandemic is caused by infection with the severe acute respiratory syndrome 2 (SARS-CoV-2), which spread primary via droplets and infects lungs. Currently widely used cell lines animals are unable to accurately mimic human physiological conditions because of abnormal status (transformed or cancer cells) species differences between humans. Organoids stem cell-derived self-organized three-dimensional culture in vitro model natural organs. Here we...
Abstract Population antibody response is thought to be important in selection of virus variants. We report that SARS-CoV-2 infection elicits a population immune mediated by lineage VH1-69 germline antibodies. A representative R1-32 from this was isolated. By cryo-EM, we show it targets semi-cryptic epitope the spike receptor-binding domain. Binding non-ACE2 competing results destruction, thereby inhibiting entry. On basis location, neutralization mechanism and analysis binding variants,...
Hepatitis B virus (HBV) infection affects hepatic metabolism. Serum metabolomics studies have suggested that HBV possibly hijacks the glycerol-3-phosphate (G3P) shuttle. In this study, two dehydrogenases (GPD1 and GPD2) in G3P shuttle were analyzed for determining their role replication findings revealed GPD2 not GPD1 inhibited replication. The knockdown of expression upregulated replication, while overexpression reduced Moreover, significantly hydrodynamic injection-based mouse models....
Vaccinia virus (VACV) confers cross-protective immunity against variola virus, the causative agent of smallpox, and has therefore been extensively exploited as a preventive vaccine. Despite global eradication smallpox in 1980s, research efforts involving VACV have continued due to potential threat being used bioweapon, which poses grave risks human health. emerged promising viral vector for vaccine development beyond traditional application prophylaxis, attributed its capacity efficient...
ABSTRACT Despite the diminishing global impact of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), virus continues to circulate and undergo mutations, posing ongoing challenges for public health. A comprehensive understanding entry mechanisms is crucial managing new epidemic strains. However, cellular processes post-endocytosis remain largely unexplored. This study employs proximity labeling examine proteins near ACE2 post-viral infection identified syntaxin-6 (STX6) as a factor...
Target recognition in high-resolution synthetic aperture radar (SAR) images is a challenging task. In this letter, novel geometrical-based SAR image simulator proposed to assist target recognition. Specifically, addition the using of Lamberitian-specular mixed model for single-bounce simulation, we propose dihedral corner double-bounce which allows directly retrieving structure information from scattering patterns. addition, tracing technique taken instead ray detection reduce computation...
SARS-CoV-2 is a betacoronavirus with single-stranded positive-sense RNA, which serious global threat to human health. Understanding the molecular mechanism of viral replication crucial for development antiviral drugs. The synthesis polyproteins step in progression. coronaviruses regulated by 5ʹ-untranslated region (UTR); however, detailed regulatory needs further investigation. present study demonstrated that RNA binding protein, RBM24, interacts genome via its recognition submotifs (RNPs)....
Low birth efficiency and developmental abnormalities in embryos derived using round spermatid injection (ROSI) limit the clinical application of this method. Further, underlying molecular mechanisms remain elusive warrant further in-depth study. In study, embryonic day (E) 11.5 mouse fetuses corresponding placentas upon ROSI, intracytoplasmic sperm (ICSI), natural vivo fertilized (control) were collected. Transcriptome DNA methylation profiles analyzed compared RNA-sequencing (RNA-seq)...
The biogenesis of covalently closed circular DNA (cccDNA) from relaxed (rcDNA) is essential for chronic hepatitis B virus (HBV) infection. Different host repair proteins are involved in the conversion rcDNA to cccDNA. Here, we reported that factor poly(ADP-ribose) polymerase 1 (PARP1) engaged HBV cccDNA formation. PARP1 depletion remarkably impaired replication and synthesis. Inhibition poly (ADP-ribosylation) activity by olaparib suppressed synthesis both vitro vivo. Specifically, early...
Abstract The coronavirus disease 2019 (COVID-19) pandemic is caused by infection with the severe acute respiratory syndrome 2 (SARS-CoV-2), which spread primary via droplets and infects lungs. Currently widely used cell lines animals are unable to accurately mimic human physiological conditions because of abnormal status (transformed or cancer cells) species differences between humans. Organoids stem cell-derived self-organized three-dimensional culture in vitro model natural organs. Here we...
Abstract Despite the extensive use of effective vaccines and antiviral drugs, chronic hepatitis B virus (HBV) infection continues to pose a serious threat global public health. Therapies with novel mechanisms action against HBV are being explored for achieving functional cure. In this study, five murine models replication were used investigate inhibitory effect RNA binding motif protein 24 (RBM24) on replication. The findings revealed that RBM24 serves as host restriction factor suppresses...
Abstract SARS-CoV-2 infection-induced hyper-inflammation links to the acute lung injury and COVID-19 severity. Identifying primary mediators that initiate uncontrolled hypercytokinemia is essential for treatments. Mast cells (MCs) are strategically located at mucosa beneficially or detrimentally regulate immune inflammations. Here we showed SARS-CoV-2-triggeed MC degranulation initiated alveolar epithelial inflammation injury. challenge induced in ACE-2 humanized mice rhesus macaques, a...
Objective To study the effects of exercises training on recovery neurological function and expression matrix metalloproteinase-9 (MMP-9) in perihematomal brain tissue after intra cerebral hemorrhage (ICH) rats. Methods Sixty-four male adult Sprague-Dawley rats were randomly divided into group control group. ICH model was induced by autobiood injection. The given balance, grasping walking every day. exercised freely standard cages. Neurological both groups measured at 3rd, 7th, 14th 21st d....