A5063917782- Animal Disease Management and Epidemiology
- Viral Infections and Immunology Research
- Viral Infectious Diseases and Gene Expression in Insects
- Vector-Borne Animal Diseases
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Gear and Bearing Dynamics Analysis
- interferon and immune responses
- Tribology and Lubrication Engineering
- Robotic Path Planning Algorithms
- Herpesvirus Infections and Treatments
- Viral Infections and Vectors
- Cell Adhesion Molecules Research
- Robotic Locomotion and Control
- Bacteriophages and microbial interactions
- Iterative Learning Control Systems
- Metallurgy and Material Forming
- Immunotherapy and Immune Responses
- Advanced Sensor and Control Systems
- Control and Dynamics of Mobile Robots
- Cancer-related molecular mechanisms research
- Advanced machining processes and optimization
- RNA Interference and Gene Delivery
- Monoclonal and Polyclonal Antibodies Research
Lanzhou Veterinary Research Institute
2016-2025
Chinese Academy of Agricultural Sciences
2016-2025
Lanzhou University
2022-2025
Xihua University
2008-2024
Gansu Provincial Center for Disease Control and Prevention
2024
University of Hong Kong
2023
Lanzhou City University
2019
Gannan Normal University
2012
Ministère de l'Agriculture et de la Souveraineté alimentaire
2009
Gansu Agricultural University
2007-2009
In this study, hollow mesoporous silica nanoparticles (HMSNs) were synthesized using the sol-gel/emulsion approach and its potential application in drug delivery was assessed. The HMSNs characterized, by transmission electron microscopy (TEM), Scanning Electron Microscopy (SEM), nitrogen adsorption/desorption Brunauer-Emmett-Teller (BET), to have a layer on surface, with an average pore diameter of about 2 nm surface area 880 m2/g. Fluorescein isothiocyanate (FITC) loaded into these used as...
Abstract We investigated the molecular epidemiology of foot-and-mouth disease virus (FMDV) serotype Asia 1, which caused outbreaks in during 2003–2007. Since 2004, region affected by this has increased from disease-endemic countries southern (Afghanistan, India, Iran, Nepal, Pakistan) northward to encompass Kyrgyzstan, Tajikistan, Uzbekistan, several regions People's Republic China, Mongolia, Eastern Russia, and North Korea. Phylogenetic analysis complete capsid protein 1 (VP1) gene...
Foot-and-mouth disease virus (FMDV) causes a severe vesicular in domestic and wild cloven-hoofed animals. Because of the limited early protection induced by current vaccines, emergency antiviral strategies to control rapid spread FMD outbreaks are needed. Here we constructed multiple microRNAs (miRNAs) targeting internal ribosome entry site (IRES) element FMDV investigated effect IRES-specific miRNAs on replication baby hamster kidney (BHK-21) cells suckling mice. Four significantly reduced...
Abstract Foot-and-mouth disease virus (FMDV) causes a highly contagious infection in cloven-hoofed animals. The format of FMD virus-like particles (VLP) as non-replicating particulate vaccine candidate is promising alternative to conventional inactivated FMDV vaccines. In this study, we explored prokaryotic system express and assemble the VLP validated potential an candidate. composed entirely (Asia1/Jiangsu/China/2005) capsid proteins (VP0, VP1 VP3) were simultaneously produced SUMO fusion...
Abstract Backgroud Porcine circovirus type 2 (PCV2) is a primary etiological agent of post-weaning multi-systemic wasting syndrome (PMWS), which disease increasing importance to the pig industry worldwide. Hollow mesoporous silica nanoparticles (HMSNs) have gained interest for use in vaccines. Methods To study potential HMSNs as protein delivery system or vaccine carriers. were synthesized by sol–gel/emulsion(oil-in-water/ethanol) method, purified PCV2 GST-ORF2-E was loaded into HMSNs, and...
Abstract Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) poses a major threat to the global swine industry, yet effective prevention control measures remain elusive. This study unveils Nitazoxanide (NTZ) as potent inhibitor of PRRSV both in vitro vivo. Through High-Throughput Screening techniques, 16 potential anti-PRRSV compounds are identified from library comprising FDA-approved pharmacopeial drugs. We show that NTZ displays strong efficacy reducing proliferation transmission...
The E2 subunit vaccine has been considered a promising alternative to an attenuated classical swine fever (CSF) vaccine. However, it fails induce good cellular immune response. Given that immunogenic adjuvants can regulate the immunity achieve maximum efficacy against antigens, immunostimulatory effects of porcine IL-28B on CSF virus (CSFV) were evaluated in present study. We expressed recombinant proteins E2-IL28B, E2, and using CHO-S mammalian cells as antigen expression platform, three...
This study delves into the antiviral efficacy of Formononetin (FMN) and Mizoribine (MZR) against Porcine Reproductive Respiratory Syndrome Virus (PRRSV), a virus with considerable economic impact current void in effective treatments. FMN MZR were found to inhibit various PRRSV strains vitro , predominantly early stages viral infection. Noteworthy was observation their synergistic effects when combined Ribavirin. The underscores potential MZR, particularly emphasizing low cytotoxicity at...
<title>Abstract</title> Recently, serotype O FMDVs belonging to four lineages (O/ME-SA/PanAsia, O/ME-SA/Ind2001, O/SEA/Mya-98 and O/Cathay) co-circulate in China the incursion of new variant virus Cathay lineage renders existing vaccines less effective, which poses a serious threat livestock industries. The surface-exposed G-H loop VP1 structural protein FMDV plays important roles for inducing neutralizing antibodies, generation antigenic attachment. Here, we generated three recombinant...
The level of neutralizing antibodies in vaccinated animals is directly related to their protection against a virus challenge. neutralization test (VNT) “gold standard” method for detecting foot-and-mouth disease (FMDV). However, VNT requires high-containment facilities that can handle live viruses and not suitable large-scale serological surveillance. In this study, bovine broadly monoclonal antibody (W145) FMDV serotype A was successfully produced using fluorescence-based single-B-cell technology.
In practical scenarios, journal bearings often exhibit shape errors due to machining imperfections and operational wear. These deviations from perfect roundness can significantly impact the performance of during start-up. This study investigates on transient mixed lubrications, such as water film temperature asperity contact, well rotor dynamics water-lubricated (WLB) at The simulation results developed numerical model are compared with experimental data existing studies verify their...
It is a great challenge to isolate the broadly neutralizing antibodies (bnAbs) against foot-and-mouth disease virus (FMDV) due its existence as seven distinct serotypes without cross-protection. Here, by vaccination of pig with FMDV O and A whole antigens, we obtained 10 bnAbs O, and/or Asia1 dissecting 216 common clonotypes two specific porcine B-cell receptor (BCR) gene repertoires containing total 12720 B cell clones, indicating induction cross-serotype after sequential antigens. The...
Foot-and-mouth disease (FMD) is the most economically important and highly contagious of cloven-hoofed animals worldwide. Control has been mainly based on large-scale vaccinations with whole-virus inactivated vaccines. In recent years, a series outbreaks type O FMD occurred in China (including Chinese Taipei, Hong Kong) posed tremendous threat to animal husbandry. Its causative agent, FMDV, evolved into three topotypes (East-South Asia (ME-SA), Southeast (SEA), Cathay (CHY)) these regions,...
Foot-and-mouth disease (FMD) is a highly contagious and economically devastating of cloven-hoofed animals in the world. The can be effectively controlled by vaccination susceptible with conventional inactivated vaccine. However, one major concern FMD virus (FMDV) vaccine that it does not allow serological discrimination between infected vaccinated animals, therefore interferes serologic surveillance epidemiology disease. A marker has proven to great value eradication control programs. In...
Abstract Background Toll-like receptor (TLR) agonists reportedly have potent antiviral and antitumor activities may be a new kind of adjuvant for enhancing immune efficacy. Resiquimod (R848) is an imidazoquinoline compound with activity functions through the TLR7/TLR8 MyD88-dependent signaling pathway. Polyinosinic-polycytidylic acid [poly(I:C)] synthetic analog double-stranded RNA that induces production pro-inflammatory cytokines by activation NF-κB TLR3. This study investigated potential...
Abstract Porcine reproductive and respiratory syndrome (PRRS) is a major threat to the global swine industry causes tremendous economic losses. Its causative agent, porcine virus (PRRSV), primarily infects immune cells, such as alveolar macrophages dendritic cells. PRRSV infection results in suppression, antibody-dependent enhancement, persistent infection. Highly pathogenic strains China cause high fever severe inflammatory responses lungs. However, pathogenesis of still not fully...
Nucleolin (NCL), a stress-responsive RNA-binding protein, has been implicated in the translation of internal ribosome entry site (IRES)-containing mRNAs, which encode proteins involved cell proliferation, carcinogenesis, and viral infection (type I IRESs). However, details mechanisms by NCL participates IRES-driven have not hitherto described. Here, we identified as protein that interacts with IRES foot-and-mouth disease virus (FMDV), is type II IRES. We also mapped interactive regions...
The development of a universal vaccine against foot-and-mouth disease virus (FMDV) is hindered by cross-serotype antigenic diversity and lack knowledge regarding neutralization the in natural hosts. In this study, we isolated serotype O-specific neutralizing antibodies (NAbs) (F145 B77) from recovered bovine hosts using single B cell antibody isolation technique. We also identified O/A cross-reacting NAb (R50) determined virus-NAb complex structures cryo-electron microscopy at near-atomic...