A5101448194- Animal Disease Management and Epidemiology
- Viral Infections and Immunology Research
- Vector-Borne Animal Diseases
- Molecular Sensors and Ion Detection
- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Luminescence and Fluorescent Materials
- Viral Infections and Vectors
- RNA regulation and disease
- interferon and immune responses
- Sulfur Compounds in Biology
- Poxvirus research and outbreaks
- Nanoplatforms for cancer theranostics
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- T-cell and Retrovirus Studies
- Advanced biosensing and bioanalysis techniques
- Viral gastroenteritis research and epidemiology
- Herpesvirus Infections and Treatments
- Complement system in diseases
- Virology and Viral Diseases
- Fluid Dynamics Simulations and Interactions
- SARS-CoV-2 and COVID-19 Research
- Cellular transport and secretion
Henan Agricultural University
2021-2025
Zhengzhou University
2024-2025
Beijing Jiaotong University
2024
China Agricultural University
2017-2021
Lanzhou Veterinary Research Institute
2014-2021
Chinese Academy of Agricultural Sciences
2014-2021
Herein a phenylselenium-substituted BODIPY (1) fluorescent turn-off sensor was developed for the purpose to achieve excellent selectivity and sensitivity H2S detection based on substitution reaction of phenylselenide group at 3-position with H2S. The excess addition hydrogen sulfide promoted further 5-position probe accompanied by decrease in fluorescence emission intensity. Sensor 1 demonstrated remarkable performance 49-fold red color intensity longer excitation wavelength, low limit...
Abstract Autophagy-related protein ATG5-ATG12 is an essential complex for the autophagophore elongation in autophagy, which has been reported to be involved foot-and-mouth disease virus (FMDV) replication. Previous reports show that positively or negatively regulates type I interferon (IFN- α / β ) pathway during infection. In this study, we found FMDV infection rapidly induced LC3 lipidation and GFP-LC3 subcellular redistribution at early stage PK-15 cells. Along with time course 2–5...
Two fluorescent, m-nitrophenol-substituted difluoroboron dipyrromethene dyes have been designed by nucleophilic substitution reaction of 3,5-dichloro-4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY). Nonsymmetric and symmetric probes, that is. BODIPY 1 (with one nitrophenol group at the position 3) 2 two groups positions 3 5) were applied to ratiometric fluorescent glutathione detection. The detection is based on two-step aromatic probes in buffer solution containing CTAB. In first stage,...
Viroporins are a family of low-molecular-weight hydrophobic transmembrane proteins that encoded by various animal viruses. form pores in host cells via oligomerization, thereby destroying cellular homeostasis and inducing cytopathy for virus replication virion release. Among the Picornaviridae viruses, 2B protein enteroviruses is well understood, whereas viroporin activity foot-and-mouth disease (FMDV) has not yet been described. An analysis FMDV domains computer-aided programs conducted...
Accumulating evidence has unveiled the roles of ribosomal proteins (RPs) belonging to large 60S subunit in regulating selective translation specific mRNAs. The specificity large-subunit RPs this process is thought provoking, given role they play canonically catalyzing peptide bond formation. Here, we have identified protein L13 (RPL13) as a critical regulator IRES-driven during FMDV infection. Our study supports model whereby IRESs recruit helicase DDX3 recognizing RPL13 facilitate...
Abstract Virus entry is an attractive target for therapeutic intervention. Here, using a combination of electron microscopy, immunofluorescence assay, siRNA interference, specific pharmacological inhibitors and dominant negative mutation, we demonstrated that the foot-and-mouth disease virus (FMDV) triggered substantial amount plasma membrane ruffling. We also found internalization FMDV induced robust increase in fluid-phase uptake virions internalized within macropinosomes colocalized with...
In addition to ribosomal protein synthesis and translation, proteins also participate in tumorigenesis tumor progression, immune responses, viral replication. Here, we show that L13 (RPL13) participates the antiviral response induced by foot-and-mouth disease virus (FMDV), inhibiting FMDV The overexpression of RPL13 promoted induction activation promoters nuclear factor-κB (NF-κB) interferon-β (IFN-β) genes, expression secretion factor IFN-β proinflammatory cytokine interleukin-6 (IL-6)....
ABSTRACT The porcine reproductive and respiratory syndrome virus (PRRSV) can lead to severe problems in sows, pneumonia weaned piglets, increased mortality, significantly negatively impacting the economy. Post-translational changes are essential for host-dependent replication long-term infection of PRRSV. Uncertainty surrounds function ubiquitin network PRRSV infection. Here, we screened 10 deubiquitinating enzyme inhibitors found that ubiquitin-specific proteinase 1 (USP1) inhibitor ML323...
African swine fever (ASF) is a highly contagious and often lethal disease of pigs caused by ASF virus (ASFV) recognized as the biggest killer in global industry. Despite exhibiting incredible self-sufficiency, ASFV remains unconditionally dependent on host translation machinery for its mRNA translation. However, less yet known regarding how ASFV-encoded proteins regulate infected cells. Here, we examined interacts with eukaryotic initiation factor 2α (eIF2α) signaling axis, which directs...
The African swine fever virus (ASFV) is a highly infectious viral pathogen that presents major threat to the global pig industry. No effective vaccine available for virus. p54 protein, structural component of ASFV, involved in adsorption and entry target cells also plays key role ASFV development disease prevention. Here, we generated species-specific monoclonal antibodies (mAbs), namely 7G10A7F7, 6E8G8E1, 6C3A6D12, 8D10C12C8 (subtype IgG1/kappa type), against protein characterized...
Abstract African swine fever (ASF) is a highly contagious and severe hemorrhagic disease caused by virus (ASFV). Currently, few safe effective vaccines or antiviral drugs are available for its prevention. Interferon (IFN), key component of innate immunity, induces interferon-stimulated genes (ISGs) activating the JAK-STAT signalling pathway, resulting in effects. ASFV strains, including SY18, HLJ18, BA71V, sensitive to IFN-I treatment; however, mechanisms which antagonizes host type I IFN...
Nucleolin (NCL), a stress-responsive RNA-binding protein, has been implicated in the translation of internal ribosome entry site (IRES)-containing mRNAs, which encode proteins involved cell proliferation, carcinogenesis, and viral infection (type I IRESs). However, details mechanisms by NCL participates IRES-driven have not hitherto described. Here, we identified as protein that interacts with IRES foot-and-mouth disease virus (FMDV), is type II IRES. We also mapped interactive regions...
Mounting evidence suggests that a number of host nuclear-resident proteins are indispensable for the replication picornaviruses, typical class cytoplasmic RNA viruses. Host nucleocytoplasmic transport is often hijacked by viruses to promote their in cytoplasm infected cells, and suppress innate immune response. However, little known about mechanisms which Senecavirus A (SVA) manipulates trafficking events infection. In this study, we combined subcellular fractionation with quantitative...
ABSTRACT African swine fever virus (ASFV) is a large double-stranded DNA virus, which the causative agent of (ASF), devastating disease suids epidemic in many countries. The has developed multiple strategies to evade surveillance from host immune system. Inflammatory responses, especially NF- κ B signaling pathway, play central roles ASFV pathogenesis and immunoevasion. In this study, we identified S273R protein (pS273R) as an antagonist canonical pathway independently its protease activity....
Proinflammatory factors play important roles in the pathogenesis of African swine fever virus (ASFV), which is causative agent (ASF), a highly contagious and severe hemorrhagic disease. Efforts prevention treatment ASF have been severely hindered by knowledge gaps viral proteins responsible for modulating host antiviral responses. In this study, we identified I10L protein (pI10L) ASFV as potential inhibitor TNF-α- IL-1β-triggered NF-κB signaling pathway, most canonical part inflammatory The...
DEAD-box helicase 23 (DDX23) is a host nuclear helicase, which part of the spliceosomal complex and involved in pre-mRNA splicing. To investigate whether DDX23, an internal ribosomal entry sites transacting factor (ITAF) affects foot-and-mouth disease virus (FMDV) replication translation through ribosome site (IRES)-dependent manner. For this, we utilized pull-down assay, Western blotting, quantitative real-time PCR, confocal microscopy, overexpression small interfering RNA knockdown, as...
Viruses deploy sophisticated strategies to hijack the host's translation machinery favor viral protein synthesis and counteract innate cellular defenses. However, little is known about mechanisms by which Senecavirus A (SVA) controls translation. Using a series of molecular cell manipulation techniques, heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1) was identified as an essential host factor involved in control SVA-infected cells. It also determined that SVA structural protein,...