A5100356839- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Animal Disease Management and Epidemiology
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Vector-Borne Animal Diseases
- Mosquito-borne diseases and control
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Immunotherapy and Immune Responses
- Hepatitis C virus research
- Monoclonal and Polyclonal Antibodies Research
- Zoonotic diseases and public health
- Viral Infections and Vectors
- interferon and immune responses
- Advanced biosensing and bioanalysis techniques
- RNA regulation and disease
- Rabies epidemiology and control
- Glycosylation and Glycoproteins Research
- MRI in cancer diagnosis
- Hepatitis B Virus Studies
- Viral Infections and Outbreaks Research
- Circular RNAs in diseases
- Parasites and Host Interactions
- RNA modifications and cancer
Third Affiliated Hospital of Southern Medical University
2025
Lanzhou Veterinary Research Institute
2015-2025
Chinese Academy of Agricultural Sciences
2015-2025
Lanzhou University
2017-2025
Henan Provincial People's Hospital
2024
Zhengzhou University
2024
Xinxiang Medical University
2024
Viva Biotech (China)
2024
Gansu Provincial Center for Disease Control and Prevention
2024
East University Of Heilongjiang
2024
ABSTRACT Foot-and-mouth disease virus (FMDV) remains a challenge for cloven-hooved animals. The currently licensed FMDV vaccines induce neutralizing antibody (NAb)-mediated protection but show defects in the early protection. Dendritic cell (DC) have shown great potency inducing rapid T-cell immunity humans and mice. Whether DC vaccination could enhance against has not been elaborately explored domestic pigs. In this study, we employed as an experimental approach to study roles of cellular...
Abstract Background The goal of this work was the development a gene targeting technology that will enable delivery therapeutic genes to brain cancer cells in vivo following intravenous administration. High‐grade gliomas overexpress epidermal growth factor receptor (EGFR) and EGFR antisense therapy could reduce EGFR‐dependent gliomas. Methods A human driven by SV40 promoter non‐viral plasmid carrying elements facilitate extra‐chromosomal replication packaged interior 85 nm pegylated...
Peripheral serotonin is involved in tumorigenesis and induces a pro-proliferative effect hepatocellular carcinoma (HCC) cells; however, the intracellular mechanisms by which exerts mitogenic remain unclear. In this research, we examined whether FOXO3a, transcription factor at interface of crucial cellular processes, plays role downstream HCC cells.The cell viability expression FOXO3a was assessed three lines (Huh7, HepG2 Hep3B) during serum deprivation presence or absence serotonin. Serum...
In addition to ribosomal protein synthesis and translation, proteins also participate in tumorigenesis tumor progression, immune responses, viral replication. Here, we show that L13 (RPL13) participates the antiviral response induced by foot-and-mouth disease virus (FMDV), inhibiting FMDV The overexpression of RPL13 promoted induction activation promoters nuclear factor-κB (NF-κB) interferon-β (IFN-β) genes, expression secretion factor IFN-β proinflammatory cytokine interleukin-6 (IL-6)....
Differentiation of 3T3‐L1 cells into adipocytes involves a highly orchestrated series complex events in which microRNAs might play an essential role. In this study, we found that the overexpression microRNA‐344 (miR‐344) inhibits cell differentiation and decreases triglyceride accumulation after MDI stimulation. We demonstrated miR‐344 directly targets 3′ UTR GSK3β (Glycogen synthase kinase 3 beta). Knockdown with siRNA results inhibiting differentiation, while its restores effect miR‐344....
Left ventricular hypertrophy (LVH) associated with hypertension and psoriasis (PSO) are linked by poor prognosis. Neutrophil extracellular traps (NETs) implicated in both conditions, but the mechanisms remain unclear. We integrated bulk single-cell RNA sequencing, mendelian randomization, immune microenvironment analysis, molecular docking to explore interactions between LVH PSO, focusing on NET-related pathways. Prediction models were also developed, gene function was validated through vivo...
A critical requirement for cancer vaccines is that they stimulate CD8+ T cell responses. In this study, we tested the ability of a polyvalent melanoma vaccine to induce responses associated antigens MAGE-3 and Melan A/MART-1. Fifteen HLA-A2+ patients with resected malignant were immunized s.c. every 2–3 weeks. cells in peripheral blood reacting HLA-A2 restricted epitopes on (FLWGPRALV) A/MART-1/(AAGIGILTV) quantitated using filter spot assay at baseline following 4 immunizations. Vaccine...
We report here the complete genomic sequence of Chinese duck flavivirus TA strain. This work is first to document this previously unknown The will help further relevant epidemiological studies and extend our general knowledge flaviviruses.
In cells, the contributions of DEAD-box helicases (DDXs), without which cellular life is impossible, are utmost importance. The extremely diverse roles nucleolar helicase DDX21, ranging from fundamental processes such as cell growth, ribosome biogenesis, protein translation, protein–protein interaction, mediating and sensing transcription, gene regulation to viral manipulation, drew our attention. We designed this project study virus–host interactions pathogenesis. A pulldown assay was used...
Nucleolin (NCL), a stress-responsive RNA-binding protein, has been implicated in the translation of internal ribosome entry site (IRES)-containing mRNAs, which encode proteins involved cell proliferation, carcinogenesis, and viral infection (type I IRESs). However, details mechanisms by NCL participates IRES-driven have not hitherto described. Here, we identified as protein that interacts with IRES foot-and-mouth disease virus (FMDV), is type II IRES. We also mapped interactive regions...
Vacuolar protein sorting 28 (Vps28), a component of the ESCRT-I (endosomal complex required for transport I), plays an important role in pathogen life cycle. Here, we investigated reciprocal regulation between Vps28 and foot-and-mouth disease virus (FMDV). Overexpression decreased FMDV replication. On contrary, knockdown increased viral Subsequently, mechanistic study showed that destabilized replication (RC) by associating with 3A rather than 2C protein. In addition, targeted VP0, VP1, VP3...
Type 2 diabetes mellitus (T2DM) is a common metabolic disease that adversely impacts patient health. In this study, T2DM model was established in ICR mice through the administration of high-sugar and high-fat diet combined with intraperitoneal injection streptozotocin to explore hypoglycemic effect polysaccharides from
ABSTRACT We sequenced the coding-complete genome sequence of bovine viral diarrhea virus (BVDV) strain NX2019/02. It belongs to 1v subgenotype. This report will increase our understanding epidemiology BVDV in China.
Background The VP1 protein of duck hepatitis A virus (DHAV) is a major structural that induces neutralizing antibodies in ducks; however, B-cell epitopes on the genotype 1 (DHAV-1) have not been characterized. Methods and Results To characterize VP1, we used monoclonal antibody (mAb) 2D10 against Escherichia coli-expressed DHAV-1. In vitro, mAb neutralized DHAV-1 virus. By using an array overlapping 12-mer peptides, found recognized phages displaying peptides with consensus motif LPAPTS....
TH(AGYLLGHINLHHLAHL(Aib)HHIL-NH 2 ), a histidine-rich, cell-penetrating peptide with acid-activated pH response, designed and synthesized by our group, can effectively target tumor tissues an acidic extracellular environment. Since the protonating effect of histidine plays critical role in acid-activated, ability TH, we series new substituents introducing electron donating groups (Ethyl, Isopropyl, Butyl) to C-2 position histidine. This resulted enhanced pH-response improved application TH...
Abstract In 2010, a pathogenic flavivirus termed duck Tembusu virus (DTMUV) caused widespread outbreak of egg-drop syndrome in domesticated ducks China. Although the glycoprotein E DTMUV is an important structural component virus, B-cell epitopes this protein remains uncharacterized. Using phage display and mutagenesis, we identified minimal epitope, 374 EXE/DPPFG 380 , that mediates binding to nonneutralizing monoclonal antibody. DTMUV-positive serum reacted with amino acid substitutions...
Introduction Foot-and-mouth disease virus (FMDV) infects the host by invading mucosal epithelial cells of respiratory or digestive tract. Therefore, establishing a specific antiviral immune barrier can effectively block viral invasion. Methods We evaluated local and systemic responses elicited intranasal immunization mice with foot-and-mouth (FMD) calcium phosphate mineralized virus-like particles (CaP-VLPs) tested whether three commercial adjuvants enhanced immunogenicity antigen. The...