A5085701136- Viral gastroenteritis research and epidemiology
- Hepatitis C virus research
- Viral Infections and Outbreaks Research
- Hepatitis B Virus Studies
- SARS-CoV-2 and COVID-19 Research
- Cancer-related molecular mechanisms research
- Viral Infections and Vectors
- Cancer-related gene regulation
- Animal Virus Infections Studies
- RNA Research and Splicing
- Virus-based gene therapy research
- COVID-19 epidemiological studies
- Cytomegalovirus and herpesvirus research
- Liver Disease Diagnosis and Treatment
- interferon and immune responses
- Viral Infections and Immunology Research
- Viral Infectious Diseases and Gene Expression in Insects
- Multi-Criteria Decision Making
- Rough Sets and Fuzzy Logic
- Clostridium difficile and Clostridium perfringens research
- Fuzzy Logic and Control Systems
- Neonatal and Maternal Infections
- Xenotransplantation and immune response
- HIV Research and Treatment
- Streptococcal Infections and Treatments
Washington University in St. Louis
2023-2025
Hubei University
2025
University of Chinese Academy of Sciences
2016-2023
Huizhou Central People's Hospital
2022
Institut Pasteur of Shanghai
2016-2021
Chinese Academy of Sciences
2017-2021
Hefei University of Technology
2021
Sun Yat-sen University
2019
Shanghai Institutes for Biological Sciences
2016
Hepatitis C virus (HCV) remains a major human pathogen that requires better understanding of virus-host interactions. In this study, we performed genome-wide CRISPR-Cas9 screening and identified TRIM26, an E3 ligase, as critical HCV host factor. Deficiency TRIM26 specifically impairs genome replication. Mechanistic studies showed interacts with HCV-encoded NS5B protein mediates its K27-linked ubiquitination at residue K51, thus promotes the NS5B-NS5A interaction. Moreover, mouse does not...
The transmission bottleneck, defined as the number of viruses shed from one host to infect another, is an important determinant rate virus evolution and level immunity required protect against transmission. Despite its importance, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) bottleneck remains poorly characterized. We adapted a SARS-CoV-2 reverse genetics system generate pool >200 isogenic harboring specific 6-nucleotide barcodes, infected donor hamsters with this pool,...
Ebola virus (EBOV) is a non-segmented, negative-sense RNA (NNSV) in the family Filoviridae, and recognized as one of most lethal pathogens planet. For viruses, cellular or virus-encoded helicases play pivotal roles viral life cycles by remodelling structures and/or unwinding dsRNA produced during replication. However, no helicase helicase-like activity has ever been found to associate with any NNSV-encoded proteins, it unknown whether replication NNSVs requires participation helicase. Here,...
Objective Despite the development of highly effective direct-acting antivirals, a prophylactic vaccine is needed for eradicating HCV. A major hurdle HCV to induce immunity against with high genome diversity. We previously demonstrated that soluble E2 (sE2) expressed from insect cells induces broadly neutralising antibodies (NAbs) and prevents infection. The objective this study develop multivalent increase antigenic coverage. Design designed trivalent containing sE2 genotype 1a, 1b 3a. Mice...
Ebola virus (EBOV) causes severe hemorrhagic fever in humans and other primates with a high case fatality rate. No approved drug or vaccine of EBOV is available, which necessitates better understanding the life cycle. Studies on have been hampered because experimentations involving live are restricted to biosafety level 4 (BSL4) laboratories. The minigenome system has provided researchers opportunity study under BSL2 conditions. Here, we developed novel replicon which, our knowledge, first...
Picobirnaviruses (PBVs) are double-stranded RNA viruses frequently detected in human and animal enteric viromes. Associations of PBVs with graft-versus-host disease type I diabetes during pregnancy have been established. Since their discovery 1988, generally assumed to be animal-infecting despite the lack culture system, model, or detection cells tissues. Recent studies proposed that bacteria fungi could hosts based on genomic analysis. Here, we functionally demonstrate multiple different...
ABSTRACT Human adenoviruses (HAdVs) are causative agents of morbidity and mortality throughout the world. These double-stranded DNA viruses phylogenetically classified into seven different species (A–G). HAdV-G52, originally isolated in 2008 from a patient presenting with gastroenteritis, is sole human-derived member G. Phylogenetic analysis previously suggested that HAdV-G52 may have simian origin, indicating potential zoonotic spillover humans. However, evidence either human or populations...
The stem-loop II motif (s2m) is an RNA structural element that found in the 3' untranslated region (UTR) of many viruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Though was discovered over 25 years ago, its functional significance unknown. In order to understand importance s2m, we created viruses with deletions or mutations s2m by reverse genetics and also evaluated a clinical isolate harboring unique deletion. Deletion mutation had no effect on growth vitro...
Purpose: Aberrant long noncoding RNA expression has been frequently reported in cancer research, including triple-negative breast (TNBC).The aim of the present study was to investigate involvement LINC00511 progression and prognosis TNBC.Materials methods: The level examined by RT-PCR TNBC tissues cell lines.MTT colony formation assays were used examine growth ability.A Boyden assay invasion ability.RNA pulldown immunoprecipitation (RIP) proteins that interacted with LINC00511.Results: We...
Ebolaviruses, such as the highly pathogenic Ebola virus (EBOV), belong to family Filoviridae and have caused outbreaks of severe fatal hemorrhagic fever over past years. Bombali (BOMV) is a newly discovered ebolavirus from bats with unknown potential infect humans. For most ebolaviruses, interaction between viral glycoprotein (GP) host receptor Niemann-Pick disease, type C1 (NPC1) crucial for entry determines tropism. Here, we analyzed BOMV GP-mediated into human cells using our recently...
Ebola virus is among the most dangerous viral pathogens, with a case fatality rate of up to 90%. Since 2013, two largest and complex outbreaks in West Africa have revealed lack investigation on this notorious virus.
Long non-coding RNA (lncRNA) nuclear-enriched abundant transcript 1 (NEAT1) exerts a pro-oncogenic role in several cancers, whereas its underlying regulatory mechanism papillary thyroid carcinoma (PTC) progression remains unknown. This research mainly explored the roles of NEAT1 PTC development. Quantitative real-time polymerase-chain reaction (qRT-PCR) was applied to measure NEAT1, miR-524-5p, and inhibitor DNA binding (ID1) expression tissues cells. Western blot conducted for detecting...
Chronic hepatitis C infection is a leading cause of liver cirrhosis, which linked to chronic hepatic inflammation. While there are multiple studies detailing the proinflammatory role interleukin-1β (IL-1β) in HCV-induced inflammasome signaling, antiviral capacity this cytokine has not been adequately investigated context HCV or other members Flaviridae. Our data indicated that IL-1β alone does inhibit replication, yet when combination with IFN-α, it can boost anti-HCV activity mediated by...
The transmission bottleneck, defined as the number of viruses that transmit from one host to infect another, is an important determinant rate virus evolution and level immunity required protect against transmission. Despite its importance, SARS-CoV-2's bottleneck remains poorly characterized, in part due a lack quantitative measurement tools. To address this, we adapted SARS-CoV-2 reverse genetics system generate pool >200 isogenic harboring specific 6-nucleotide barcodes inserted ORF10,...
The stem-loop II motif (s2m) is a RNA structural element that found in the 3' untranslated region (UTR) of many viruses including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Though was discovered over twenty-five years ago, its functional significance unknown. In order to understand importance s2m, we created with deletions or mutations s2m by reverse genetics and also evaluated clinical isolate harboring unique deletion. Deletion mutation had no effect on growth vitro ,...