A5043986098- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Plant Virus Research Studies
- Insect symbiosis and bacterial influences
- Vector-Borne Animal Diseases
- Rabies epidemiology and control
- Animal Disease Management and Epidemiology
- Advanced Neural Network Applications
- Domain Adaptation and Few-Shot Learning
- Viral Infections and Outbreaks Research
- Invertebrate Immune Response Mechanisms
- Virology and Viral Diseases
- Mathematical and Theoretical Epidemiology and Ecology Models
- Adversarial Robustness in Machine Learning
Wuhan Institute of Virology
2021-2024
Chinese Academy of Sciences
2021-2024
University of Chinese Academy of Sciences
2021-2024
Abstract Aedes aegypti is a primary vector for transmitting various arboviruses, including Yellow fever, dengue and Zika virus. The mosquito midgut the principal organ blood meal digestion, nutrient absorption initial site of arbovirus infection. Although previous study delineated midgut’s transcriptome Ae. at single-nucleus resolution, there still lacks an established protocol isolating RNA sequencing single cells midgut, which required investigating arbovirus-midgut interaction single-cell...
Abstract Arthropod-borne virus (arbovirus) and arthropod-specific (ASV) are viruses circulating amongst hematophagous arthropods that broadly transmitted in ecological systems. Arbovirus may replicate both vertebrates invertebrates some known to be pathogenic animals or humans. ASV only invertebrate yet they basal many types of arboviruses. We built a comprehensive dataset arbovirus by curating globally available data from the Catalog, list Section VIII-F Biosafety Microbiological Biomedical...
Biting midges are one of the most common hematophagous insects. They capable transmitting a wide range arboviruses and have significant impact on public health veterinary medicine. Herein, from midge samples collected in 2013 Yunnan, China, sample induced cell cytopathic effect (CPE) BHK-21, MA104, PK15 lines. Next-generation sequencing data, RACE PCR determined genome sequence designated as an Oya virus (OYAV) isolate SZC50. Phylogenetic analysis revealed that it was cluster into viruses...
Objective: Mosquitoes are medically important arthropod vectors that harbor a variety of viruses. Geography and climate known to be associated with variations in mosquito density, species viromes. Our study investigated the dynamic changes populations, compositions viromes regularly disinfected environment Wuhan, China, during 2020. Methods: Traps were set different habitats, including an urban residential area, two hospitals, scenic area pig farm rural region between April October The...
The escalation of viruses over the past decade has highlighted need to determine their respective hosts, particularly for emerging ones that pose a potential menace welfare both human and animal life. Yet, traditional means ascertaining host range viruses, which involves field surveillance laboratory experiments, is laborious demanding undertaking. A computational tool with capability reliably predict ranges novel can provide timely responses in prevention control infectious diseases....
We conducted a large-scale survey of arboviruses in mosquitoes and biting midges to assess the presence spread mosquito-borne pathogens currently circulating on Hainan Island, China. RT-PCR assays were used detect arbovirus species, distribution, infection rates midges. Cell inoculation high throughput sequencing performed isolate viruses assemble full viral genomes. Phylogenetic analysis was identify genotypes evolutionary relationships with known viruses. During 2019-2023, 32,632 21,000...
Oya virus (OYAV) and Ebinur lake (EBIV) belong to the genus Orthobunyavirus within Peribunyaviridae family, both are recognized as novel with potential threat animal or public health. Given their cause outbreaks detection in diverse samples across different regions, need for a reliable efficient molecular method OYAV EBIV becomes imperative. The S-segment of was used designing specific primer probe sets, which were employed real-time reverse transcription quantitative PCR (RT-qPCR) assay....
Mosquito-borne viruses (MBVs), which are infectious pathogens to vertebrates, spread by many mosquito species, posing a severe threat public health. Once ingested, the must overcome midgut barrier reach hemolymph, from where they might potentially salivary glands. When bites, these new vertebrate hosts. Similarly, may pick up different viruses. In general, only tiny portion of enter glands via gut. The transmission efficiency is be affected two physical barriers found in species: and...
Mosquito-specific flaviviruses comprise a group of insect-specific viruses with single positive RNA, which can affect the duplication mosquito-borne and life growth mosquitoes, have potential to be developed as vaccine platform for viruses. In this study, strain mosquito flavivirus (MFV) YN15-283-02 was detected in Culicoides collected from Yunnan, China. The isolation purified MFV cell culture failed, virus then rescued by an infectious clone. To study biological features vitro vivo,...
Abstract Orthobunyaviruses have been reported to cause severe diseases in humans or animals, posing a threat human health and social economy. Ebinur lake virus (EBIV) is newly classified orthobunyavirus, which needs further intensive study therapies cope with its potential infection risk animals. Here, through the reverse genetics system, recombinant EBIV of wild type (rEBIV/WT) NP-conjugated-eGFP (rEBIV/eGFP/S) were rescued for application rapid antiviral drug screening. The eGFP...
Abstract Mosquitoes are medically important arthropod vectors and harbor a great variety of viruses. The population density, species virome mosquitoes varies according to geography climate. To investigate the dynamic changes in composition diversity their viromes Wuhan, China, total 2,345 adult collected from different habitats including an urban residential area, two hospitals, scenic pig farm rural region April October 2020 were subjected morphological identification, RT-qPCR metagenomic...