A5100347714- Building Energy and Comfort Optimization
- Wind and Air Flow Studies
- Aerodynamics and Fluid Dynamics Research
- Mosquito-borne diseases and control
- Vehicle emissions and performance
- Viral Infections and Vectors
- Monoclonal and Polyclonal Antibodies Research
- Vascular Malformations Diagnosis and Treatment
- Intracerebral and Subarachnoid Hemorrhage Research
- Intracranial Aneurysms: Treatment and Complications
- Air Quality and Health Impacts
- HIV Research and Treatment
- Insect symbiosis and bacterial influences
- Infection Control and Ventilation
- Myasthenia Gravis and Thymoma
- Malaria Research and Control
- Wnt/β-catenin signaling in development and cancer
- Enzyme Structure and Function
- Urban Heat Island Mitigation
- Dermatological and Skeletal Disorders
- Refrigeration and Air Conditioning Technologies
- Entomopathogenic Microorganisms in Pest Control
- Noise Effects and Management
- Cancer-related gene regulation
- Insect Resistance and Genetics
Chinese Academy of Medical Sciences & Peking Union Medical College
2020-2025
Fudan University
2020-2025
Eye & ENT Hospital of Fudan University
2017-2025
Tsinghua University
2016-2025
Sun Yat-sen University
2025
Fifth Affiliated Hospital of Sun Yat-sen University
2025
Southwest Medical University
2025
Anhui University of Science and Technology
2022-2024
State Key Laboratory of Building Safety and Built Environment
2018-2024
Second Affiliated Hospital of Guangzhou Medical University
2024
Little is known about how the immune microenvironment of breast cancer evolves during disease progression.We compared tumor infiltrating lymphocyte (TIL) count, programmed death-ligand 1 (PD-L1) protein expression by immunohistochemistry and mRNA levels 730 immune-related genes using Nanostring technology in primary metastatic samples.TIL counts PD-L1 positivity were significantly lower metastases. Immune cell metagenes corresponding to CD8, T-helper, T-reg, Cytotoxic T, Dendritic Mastoid...
SUMMARY A new coronavirus (CoV) identified as COVID-19 virus is the etiological agent responsible for 2019-2020 viral pneumonia outbreak that commenced in Wuhan 1–4 . Currently there no targeted therapeutics and effective treatment options remain very limited. In order to rapidly discover lead compounds clinical use, we initiated a program of combined structure-assisted drug design, virtual screening high-throughput identify leads target main protease (M pro ). M key CoV enzyme, which plays...
A human monoclonal antibody designated 15e is reactive with the envelope glycoprotein (gp120) of multiple isolates immunodeficiency virus type 1 (HIV-1). Antibody also neutralizes HIV-1 broad specificity and blocks gp120 binding to CD4. Characterization epitope shows that it conformation dependent distinct from previously recognized functional domains gp120, suggesting this represents a novel site important for neutralization CD4 binding. These findings have implications development vaccine AIDS.
Geese were domesticated over 6,000 years ago, making them one of the first poultry. are capable rapid growth, disease resistance, and high liver lipid storage capacity, can be easily fed coarse fodder. Here, we sequence analyze whole-genome an economically important goose breed in China compare it with that terrestrial bird species.A draft whole-goose genome was obtained by shotgun sequencing, 16,150 protein-coding genes predicted. Comparative genomics indicate significant differences occur...
Panax notoginseng (Burk.) F.H. Chen is one of the most highly valued medicinal plants in world. The major bioactive molecules are triterpene saponins, which also known as ginsenosides. However, its large genome size has hindered assembly a draft by whole sequencing. Hence, genomic and transcriptomic details about P. notoginseng, especially biosynthetic pathways gene expression different parts plant, have remained largely unknown until now. In this study, RNA sequencing three tissues was...
Mosquito-borne flaviviruses infect both mammals and mosquitoes. RNA interference (RNAi) has been demonstrated as an anti-flavivirus mechanism in mosquitoes; however, whether how induce antagonize RNAi-mediated antiviral immunity remains unknown. We show that the nonstructural protein NS2A of dengue virus-2 (DENV2) act a viral suppressor RNAi (VSR). When NS2A-mediated suppression was disabled, resulting mutant DENV2 induced Dicer-dependent production abundant DENV2-derived siRNAs...
A panel of seven monoclonal antibodies against the relatively conserved CD4-binding domain on human immunodeficiency virus type 1 (HIV-1) gp120 was generated by immunizing mice with purified gp120. These reacted specifically in an enzyme-linked immunosorbent assay and Western blots (immunoblots). By using synthetic peptides as antigens assay, epitopes these were mapped to amino acid residues 423 437 Further studies radioimmunoprecipitation assays showed that they cross-reacted both gp160...
Viral replicase recruitment and long-range RNA interactions are essential for virus replication, yet the mechanism of their interplay remains elusive. Flaviviruses include numerous important human pathogens, e.g., dengue (DENV) Zika (ZIKV). Here, we revealed a highly conserved, conformation-tunable cis-acting element named 5'-UAR-flanking stem (UFS) in flavivirus genomic 5' terminus. We demonstrated that UFS was critical efficient NS5 viral synthesis different flaviviruses. Interestingly,...
Using a convergent synthetic route to enable multiple points of diversity, series glucocorticoid receptor modulators (GRM) were profiled for potency, selectivity, and drug-like properties in vitro. Despite covering large range profiling the nonconjugated small molecule was suboptimal they conjugated mouse antitumor necrosis factor (TNF) antibody using MP-Ala-Ala linker. Screening resulting drug conjugates (ADCs) provided better assessment efficacy physical properties, reinforcing need...
To define the domains in envelope glycoprotein important for antibody neutralization of human immunodeficiency virus type 1 (HIV-1), monoclonal antibodies (mAbs) were generated by immunizing mice with purified gp120 IIIB isolate. One mAb, G3-4, reacted homologous (IIIB) and heterologous (RF) isolates. In addition, mAb G3-4 efficiently neutralized both RF viruses vitro, as well four nine primary HIV-1 competition immunoassays, soluble CD4 found to inhibit one another binding gp120. However,...
Mutations in Wnt receptor LRP5/6 and polymorphism Wnt-regulated transcription factor TCF7L2 are associated with dysregulation of glucose metabolism. However, it is not clear whether antagonist Dickkopf (Dkk) has a significant role the regulation Here, we identified small-molecule inhibitors Dkk through molecular modeling, computation-based virtual screens, biological assays. One reduced basal blood-glucose concentrations improved tolerance mice. This inhibitor appeared to act DKK2 because...
Despite substantial efforts to control and contain H5N1 influenza viruses, bird flu viruses continue spread evolve. Neutralizing antibodies against conserved epitopes on the viral hemagglutinin (HA) could confer immunity diverse virus strains provide information for effective vaccine design. Here, we report characterization of a broadly neutralizing murine monoclonal antibody, H5M9, most clades subclades that was elicited by immunization with HA A/Goose/Guangdong/1/96 (H5N1), immediate...
Mutations in the essential adaptor proteins CCM2 or CCM3 lead to cerebral cavernous malformations (CCM), vascular lesions that most frequently occur brain and are strongly associated with hemorrhagic stroke, seizures, other neurological disorders. binds CCM3, but molecular basis of this interaction, its functional significance, have not been elucidated. Here, we used x-ray crystallography structure-guided mutagenesis show an α-helical LD-like motif within highly conserved “HP1” pocket focal...