A5007523102- Wound Healing and Treatments
- Reconstructive Surgery and Microvascular Techniques
- Surgical Sutures and Adhesives
- Burn Injury Management and Outcomes
- Virus-based gene therapy research
- Reconstructive Facial Surgery Techniques
- Dermatologic Treatments and Research
- Diabetic Foot Ulcer Assessment and Management
- Electrospun Nanofibers in Biomedical Applications
- RNA Interference and Gene Delivery
- Mesenchymal stem cell research
- Pressure Ulcer Prevention and Management
- Corneal Surgery and Treatments
- Silk-based biomaterials and applications
- Surgical site infection prevention
- Organ and Tissue Transplantation Research
- Facial Nerve Paralysis Treatment and Research
- Tissue Engineering and Regenerative Medicine
- Periodontal Regeneration and Treatments
- Breast Implant and Reconstruction
- Planarian Biology and Electrostimulation
- Antimicrobial Peptides and Activities
- Tendon Structure and Treatment
- Connective Tissue Growth Factor Research
- Facial Trauma and Fracture Management
Applied Tissue Technologies (United States)
2017-2025
Brigham and Women's Hospital
2011-2024
Harvard University
2015-2024
University of Pittsburgh
2023
Lemuel Shattuck Hospital
2023
United States Army Institute of Surgical Research
2022
The University of Texas at San Antonio
2022
University of Michigan
2022
Odense University Hospital
2017
In-Q-Tel
2014-2016
Background: Acellular dermal matrix has been popularized as an adjunct to tissue expander or implant breast reconstruction given its utility in providing additional coverage and support for the inferior pole. This study was performed assess risk of postoperative complications associated with use acellular matrix–assisted implant-based reconstruction. Methods: The authors a retrospective analysis consecutive immediate reconstructions over 6-year period. A total 415 were divided into two...
Significance: A moist wound environment has several benefits that result in faster and better quality of healing. It facilitates autolytic debridement, reduces pain, scarring, activates collagen synthesis, promotes keratinocyte migration over the surface, supports presence function nutrients, growth factors, other soluble mediators microenvironment. Recent Advances: Wound dressings can be utilized to create, maintain, control a for Moist divided into films, foams, hydrocolloids, hydrogels,...
Unlike conventional reconstruction, facial transplantation seeks to correct severe deformities in a single operation. We report on three patients who received full-face transplants at our institution 2011 operations that aimed for functional restoration by coaptation of all main available motor and sensory nerves. enumerate the technical challenges postoperative complications their management, including episodes acute rejection two patients. At 6 months follow-up, allografts were surviving,...
Abstract The Wound Healing Foundation (WHF) recognised a need for an unbiased consensus on the best treatment of chronic wounds. A panel 13 experts were invited to virtual meeting which took place 27 March 2021. proceedings organised in sub‐sections diagnosis, debridement, infection control, dressings, grafting, pain management, oxygen treatment, outcomes and future needs. Eighty percent or better concurrence among panellists was considered consensus. large number critical questions...
Abstract Wound microenvironment plays a major role in the process of wound healing. It contains various external and internal factors that participate pathophysiology. The pH is an important factor influences healing by changing throughout process. Several previous studies have investigated relation to pathogens but concentrating on effects itself are inconclusive. purpose this study was comprehensively controlled fashion investigate effect studying its human primary keratinocyte fibroblast...
Cell surface heparan sulfate proteoglycans, such as the syndecans, are required for cellular responses to heparin-binding growth factors and extracellular matrix components. Expression of syndecan-1 -4 is induced in mesenchymal cells during wound repair mouse, consistent with a role syndecans regulating cell proliferation migration response these effectors. Here we show that fluid contains inductive activity mimics vivo induction time appearance, specificity cells, selectivity -4. We have...
Wound fluid was obtained from porcine partial-thickness excisional wounds and analyzed for heparin-binding growth factors. Two factor activities were detected, a relatively minor one that eluted heparin affinity column with 0.65 M NaCl major 1.1 NaCl. These not present in wound 1 hr after injury but appeared day injury, maximal 2-3 days detectable by 8 injury. The identified as platelet-derived (PDGF)-like activity the use of specific anti-PDGF neutralizing antibodies. shown to be...
Proteoglycans have been shown <i>in vitro</i> to bind multiple components of the cellular microenvironment that function during wound healing. To study composition and these molecules when derived from an<i>in vivo</i> source, soluble proteoglycans released into human fluid were characterized evaluated for influence on fibroblast growth factor-2 activity. Immunoblot analysis revealed presence syndecan-1, syndecan-4, glypican, decorin, perlecan, versican. Sulfated glycosaminoglycan...
A study was undertaken to determine the cosmetic and functional problems associated with latissimus dorsi muscle donor site. Twenty-four patients undergoing both free pedicle myocutaneous flap procedures for a wide variety of reconstructive were studied. All had contour defect at site, scar which varied patient's age whether overlying skin been taken flap. Mild moderate shoulder weakness some loss motion noted in most improved over course several months. An upper extremity disability...
Abstract In cats anesthetized with chloralose the lengths and diameters of different vascular segments in tenuissimus muscle were measured vital microscope. The flow characteristics, including red cell velocity, registered. Fixed stained specimens analyzed histologically as well Indian ink‐perfused muscles treated according to method Spalteholz. average fibre diameter was 44 μm. 62% fibres (48% B‐fibres, 41 μm 14% C‐fibres, 26 μm) remaining 38% white (A‐fibres, 55 μm). surrounded by 3.6...
Abstract Background Infected wounds present a major complication in patients with diabetes. Staphylococcus aureus is the most common single isolate diabetic wounds. Human beta‐defensin (hBD)‐3 antimicrobial active and appears to play key role immune response. The study aimed analyse effect of hBD‐3 expression model infected Methods Excisional were created on backs Yorkshire pigs Ad5‐CMV‐hBD‐3 vectors microseeded. Wounds inoculated S. , covered polyurethane chamber analysed for transgene...
Abstract Background Wound infection is a common complication in diabetic patients. The progressive spread of infections and development drug-resistant strains underline the need for further insights into bacterial behavior host order to develop new therapeutic strategies. aim our study was large animal model suitable monitoring effect wounds. Methods Fourteen excisional wounds were created on dorsum non-diabetic Yorkshire pigs sealed with polyurethane chambers. Wounds either inoculated 2 ×...
Composite facial allotransplantation is emerging as a treatment option for severe disfigurements. The technical feasibility of transplantation has been demonstrated, and the initial clinical outcomes have encouraging. We report an excellent functional anatomical restoration 1 year after face transplantation. A 59-year-old male with disfigurement from electrical burn injury was treated allograft composed bone soft tissues to restore midfacial form function. An potent antirejection tapered...
Full-thickness skin wounds are preferably allowed to heal under controlled hydration dressings such as hydrocolloids. It was hypothesized that a wet (liquid) environment rather than dry or moist one would accelerate the wound healing process. We compared repair by secondary intention in full-thickness (saline), (hydrocolloid), and (gauze) conditions an established porcine model. The study included three animals with total of 70 layered standardized fashion on back young Yorkshire pigs....
Effects of wet (saline in a vinyl chamber), moist (hydrocolloid dressing), and dry (sterile gauze dressing) environments on wound repair were studied porcine partial-thickness model. Chambers exchanged refilled daily with normal saline containing penicillin G (100 U/ml) streptomycin µg/ml). Hydrocolloid dressings kept place until biopsy the site. Wounds wet, moist, completely epithelialized days 6, 7, 8, respectively. Thickness epidermis wounds was 204 ± 23, 141 12, 129 18 (mean SEM), Moist...
Full-thickness skin wounds are preferably allowed to heal under controlled hydration dressings such as hydrocolloids. It was hypothesized that a wet (liquid) environment rather than dry or moist one would accelerate the wound healing process. We compared repair by secondary intention in full-thickness (saline), (hydrocolloid), and (gauze) conditions an established porcine model. The study included three animals with total of 70 layered standardized fashion on back young Yorkshire pigs....
This study retrospectively identifies and characterizes patients with facial palsy related to birth trauma describes the natural history of this disorder. The records infants born weakness or paralysis over a 5-year period at Brigham Women's Hospital were reviewed, criteria defined assign diagnosis acquired based on documented physical examinations. majority followed up by interview family member. Among 44,292 between October 1, 1982 July 31, 1987, there 92 recorded cases congenital seventh...