A5015760095- Urological Disorders and Treatments
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Pediatric Urology and Nephrology Studies
- Wound Healing and Treatments
- Sexual Differentiation and Disorders
- Urinary and Genital Oncology Studies
- Mesenchymal stem cell research
- Health Sciences Research and Education
- Childhood Cancer Survivors' Quality of Life
- Urologic and reproductive health conditions
- Testicular diseases and treatments
- Renal and related cancers
- Bladder and Urothelial Cancer Treatments
- Meta-analysis and systematic reviews
- COVID-19 and healthcare impacts
- Congenital Anomalies and Fetal Surgery
- Silk-based biomaterials and applications
- Adolescent and Pediatric Healthcare
- Periodontal Regeneration and Treatments
- Health and Medical Research Impacts
- Bone Tissue Engineering Materials
- Advanced Mathematical Theories
- Reconstructive Surgery and Microvascular Techniques
- Child and Adolescent Health
University of Copenhagen
2019-2025
Rigshospitalet
2019-2025
Karolinska Institutet
2016-2025
Copenhagen University Hospital
2019-2025
Norwegian University of Science and Technology
2025
Karolinska University Hospital
2012-2024
BioClinicum (Russia)
2024
Akademiska Barnsjukhuset
2023
Johns Hopkins University
2022
Howard University
2020
In 2016, guidelines for diagnostic Next Generation Sequencing (NGS) have been published by EuroGentest in order to assist laboratories the implementation and accreditation of NGS a setting. These mainly focused on Whole Exome (WES) targeted (gene panels) sequencing detecting small germline variants (Single Nucleotide Variants (SNVs) insertions/deletions (indels)). Since then, Genome (WGS) has increasingly introduced diagnosis rare diseases as WGS allows simultaneous detection SNVs,...
Abstract Background Infected wounds present a major complication in patients with diabetes. Staphylococcus aureus is the most common single isolate diabetic wounds. Human beta‐defensin (hBD)‐3 antimicrobial active and appears to play key role immune response. The study aimed analyse effect of hBD‐3 expression model infected Methods Excisional were created on backs Yorkshire pigs Ad5‐CMV‐hBD‐3 vectors microseeded. Wounds inoculated S. , covered polyurethane chamber analysed for transgene...
Abstract Background Wound infection is a common complication in diabetic patients. The progressive spread of infections and development drug-resistant strains underline the need for further insights into bacterial behavior host order to develop new therapeutic strategies. aim our study was large animal model suitable monitoring effect wounds. Methods Fourteen excisional wounds were created on dorsum non-diabetic Yorkshire pigs sealed with polyurethane chambers. Wounds either inoculated 2 ×...
ABSTRACT Diabetic wounds result in significant morbidity, prolonged hospitalization, and enormous health‐care expenses. Pigs have been shown to wound healing resembling that humans. The aim of this study was develop a large‐animal model for diabetic healing. Diabetes induced by streptozotocin injection Yorkshire pigs. Full‐thickness were created dressed with sealed chamber. Nondiabetic pigs or without high glucose fluid concentration served as controls. Glucose serum measured collected....
We present data on long-term functional and cosmetic results after hypospadias surgery.Males older than 18 years with treated in Sweden were asked to participate the study, as well age matched controls circumcised men. All participants answered questionnaires, a subgroup was examined during an outpatient visit. Relationships outcome analyzed using analysis of variance regression analysis.A total 167 patients mean 34 169 33 questionnaire. Of 63% had distal, 24% mid 13% proximal hypospadias. A...
Tissue engineering has not been widely adopted in clinical settings for several reasons, including technical challenges, high costs, and regulatory complexity. Here, we introduce the Perioperative Layered Autologous Expansion graft (PLATE graft), a composite biomaterial collagen-reinforced construct with autologous epithelium on one side smooth muscle tissue other. Designed to mimic structure function of natural hollow organs, PLATE is unique that it can be produced standard operating...
Abstract Urogenital reconstructive malformation surgery is sometimes hampered by lack of tissue for the repair. We have previously shown that autologous micrografting allows single-staged scaffold cellularization after surgical implantation. Here, a collagen-based reinforced with biodegradable mesh and stent was implanted as bladder conduit in ten full-grown female minipigs. aimed to assess short-term regenerative outcomes, safety, feasibility implanting tubular urinary micrografted...
To evaluate the long-term effects on hypospadias repair with cultured autologous urothelial cells.From 2000 to 2002, six patients scrotal or perineal and pronounced chordee were treated surgically cell transplants. All evaluated at 6-8 years postoperatively, that is, in prepubertal period. The outcome was assessed respect cosmetic appearance, voiding function, urinary flow, artificial erection, urethroscopy biopsies.Median follow-up time 7.25 years. Up date, all present a good appearance....
Summary The aim of this study was to investigate sexual function and fertility in adult men born with hypospadias. Patients hypospadias, age‐matched controls, a group circumcised completed questionnaire constructed reflect their psychosexual situation fertility. Core gender identity, orientation, role behavior also assessed. 167 patients [63% distal, 24% mid shaft 13% proximal, mean age 34 (19–54) years], 169 controls from the general population [mean 33 (19–48) years] 47 because phimosis...
We present a method for producing cell–scaffold hybrid construct at the bedside. The is composed of plastic-compressed collagen together with poly(ɛ-caprolactone) (PCL)-knitted mesh that yields an integrated, natural–synthetic scaffold. This was evaluated by seeding minced bladder mucosa, followed proliferation in vitro. High mechanical strength combination biological environment suitable tissue growth achieved through creation showed increased tensile (17.9±2.6 MPa) when compared to...
Posterior urethral valves (PUV) account for 17% of paediatric end-stage renal disease. A major issue in the management PUV is prenatal prediction postnatal function. Fetal ultrasound and fetal urine biochemistry are currently employed this prediction, but clearly lack precision. We previously developed a peptide signature that predicted utero with high precision function fetuses PUV. describe here objectives design prospective international multicentre ANTENATAL (multicentre validation...
One of the concerns surrounding cryptorchidism is risk impaired fertility. Current guidelines recommend orchiopexy at age 6 to 12 months optimize fertility outcome. We evaluated potential boys with nonsyndromic who underwent within recommended range clarify need for eventual supplemental treatment modalities.We retrospectively mini-puberty hormones (follicle-stimulating hormone, luteinizing hormone and inhibin B) testicular biopsies from first year life between 2010 2019. histologically...
Abstract This study explores a novel approach to vaginal reconstructive surgery using autologous tissue grafts, which may provide new therapeutic options for women with congenital or acquired anomalies. Using small segment, we engineered six-fold expanded graft perioperatively, leveraging the body as bioreactor and avoiding preoperative culture. In adolescent White New Zealand rabbits, defect was created repaired PLATE (perioperative, layered, autologous, tissue-expansion graft) containing...
Abstract Background Impaired wound healing is a frequent phenomenon in diabetes mellitus. However, little known of the fundamental cause this pathology. The present study examined effect human insulin‐like growth factor (hIGF)‐1 overexpression combination with autologous cell transplantation to diabetic wounds preclinical large‐animal model. Methods Diabetes was induced Yorkshire pigs streptozotocin. Keratinocytes were cultured and transfected hIGF‐1 or LacZ transgene. Plasmids lipoplexed...