A5012176481- Diabetes Management and Research
- Animal Disease Management and Epidemiology
- T-cell and Retrovirus Studies
- Monoclonal and Polyclonal Antibodies Research
- Diabetes Treatment and Management
- HIV Research and Treatment
- Prostate Cancer Treatment and Research
- Diabetes and associated disorders
- Vector-Borne Animal Diseases
- Pancreatic function and diabetes
- HIV/AIDS drug development and treatment
- Glycosylation and Glycoproteins Research
- CAR-T cell therapy research
- Diabetes Management and Education
- Transgenic Plants and Applications
- Medication Adherence and Compliance
- Protein purification and stability
- Viral Infectious Diseases and Gene Expression in Insects
- Adolescent and Pediatric Healthcare
- Long-Term Effects of COVID-19
- Cancer Immunotherapy and Biomarkers
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Neuroendocrine regulation and behavior
Ansun BioPharma (United States)
2022-2025
Children's Hospital of Los Angeles
2012-2024
University of Southern California
2012-2024
Southern California University for Professional Studies
2012-2023
Seagen (United States)
2022
Northwest University
2018
University of Washington
2018
Washington University in St. Louis
2018
University of Colorado Denver
2017
SUNY Upstate Medical University
2017
It is generally believed that the gag gene product of human immunodeficiency virus type 1 (HIV-1) processed into several core proteins by a virus-specific protease. We used deletion mutation analysis to study role HIV-specific protease in processing and its requirement for viral infectivity. Several mutant genomes with deletions were constructed. A mammalian cell line, COS-M6, transfected wild-type genome was shown produce virions containing proteins, while COS-M6 cells two mutated could...
By analogy to other retroviruses, the major envelope glycoprotein, gp120, of human T-lymphotropic virus type III (HTLV-III) is a probable target for neutralizing antibody. This antigen has been purified from H9 cells chronically infected with HTLV-IIIB prototype strain. Several goats immunized gp120 produced antibodies that neutralized infection by homologous isolate. These same sera failed neutralize divergent HTLV-IIIRF Individuals HTLV-III commonly develop could be isolated using coupled...
OBJECTIVE The objective of the study was to report on measurement properties revised and updated Pediatric Quality Life Inventory (PedsQL) 3.2 Diabetes Module for children, adolescents, young adults with type 1 diabetes. RESEARCH DESIGN AND METHODS 33-item PedsQL Generic Core Scales were completed in a 10-site national field test by 656 families patients ages 2–25 years RESULTS 15-item Symptoms Summary Score 18-item Management derived from factor analysis items. Scores evidenced excellent...
We have cloned the genomic DNA fragments encoding heavy and light chain variable regions of monoclonal antibody 17-1A, we inserted them into mammalian expression vectors containing segments human gamma 3 kappa constant regions. The transfer these mouse-human chimeric immunoglobulin genes Sp2/0 mouse myeloma cells resulted in production functional IgG that retained specific binding to surface antigen 17-1A expressed on colorectal carcinoma cells.
A panel of seven monoclonal antibodies against the relatively conserved CD4-binding domain on human immunodeficiency virus type 1 (HIV-1) gp120 was generated by immunizing mice with purified gp120. These reacted specifically in an enzyme-linked immunosorbent assay and Western blots (immunoblots). By using synthetic peptides as antigens assay, epitopes these were mapped to amino acid residues 423 437 Further studies radioimmunoprecipitation assays showed that they cross-reacted both gp160...
We have identified an immune activation gene, denoted Act-2, by differential hybridization screening of activated T-cell library. The gene is induced rapidly after with phytohemagglutinin, B-cell Staphylococcus aureus Cowan I, and monocyte lipopolysaccharide. isolated a cDNA containing the full-length coding region. deduced amino acid sequence predicts open reading frame 92 acids, including very hydrophobic N terminus, which weight matrix score predicted to be signal peptide. Using...
TODAY (Treatment Options for type 2 Diabetes in Adolescents and Youth) is a federally funded multicenter randomized clinical trial comparing three treatments of youth onset diabetes.
OBJECTIVE Obese youth without diabetes with monophasic oral glucose tolerance test (OGTT) response curves have lower insulin sensitivity and impaired β-cell function compared those biphasic curves. The OGTT curve has not been studied in youth-onset type 2 diabetes. Here we the hypothesis that at randomization TODAY (Treatment Options for Type Diabetes Adolescents Youth) study forecasts heightened glycemic failure rates accelerated decline function. RESEARCH DESIGN AND METHODS OGTTs (n = 662)...
First- and secondhand smokers are at an increased risk for influenza virus (IFV)-related respiratory failure death. Despite approved antiviral treatments, there is unmet need treatments that can improve outcomes in populations failure, including tobacco users with Chronic Obstructive Pulmonary Disease (COPD). Here we show the sialidase fusion protein, DAS181, reduced viral burden, mitigated inflammation, attenuated lung function loss, consistent broad-spectrum anti-influenza responses a...
Murine mAb BAT123, which was made against the envelope glycoprotein gp120 of HTLV-IIIB strain HIV type 1 (HIV-1), is capable neutralizing in vitro. It also inhibits fusion between uninfected CD4+ cells and HIV-1-infected to form syncytia. As a step explore potential utility anti-HIV antibody vivo, we have constructed mouse-human chimeric by rDNA techniques. The antibody, bears variable domains mouse BAT123 constant Cr1 C kappa human Ig retains Ag specificity as determined its reactivity with...
Human T-cell lymphotropic virus type III (HTLV-III), the causative agent of acquired immune deficiency syndrome (AIDS), was recently isolated and its genomic structure analyzed by DNA cloning methods. In studies reported here a combined expression system used to identify HTLV-III encoded peptides that react immunologically with antibodies in sera from AIDS patients. Cloned sheared into approximately 500-base-pair fragments inserted an "open reading frame" vector, pMR100. The expressed...
The genome of human T-cell lymphotropic virus type III/lymphadenopathy-associated (HTLV-III/LAV), the infectious agent etiologically associated with acquired immunodeficiency syndrome, contains, in addition to genes for polymerase, core, and envelope proteins, several open reading frames. To investigate whether 3' frame (3' orf) located between gene long terminal repeat is a expressed vivo infected individuals, we inserted fragment orf prokaryotic expression vector. protein product...