A5019801137- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 and COVID-19 Research
- Protein purification and stability
- Viral Infectious Diseases and Gene Expression in Insects
- Transgenic Plants and Applications
- CAR-T cell therapy research
- Glycosylation and Glycoproteins Research
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- Advanced Biosensing Techniques and Applications
- Biosensors and Analytical Detection
- Immunotherapy and Immune Responses
- Bacteriophages and microbial interactions
- Toxin Mechanisms and Immunotoxins
- Advanced biosensing and bioanalysis techniques
- Metabolomics and Mass Spectrometry Studies
- Viral Infections and Immunology Research
- Microbial Metabolic Engineering and Bioproduction
- Biosimilars and Bioanalytical Methods
- Microbial bioremediation and biosurfactants
- Bacillus and Francisella bacterial research
- Animal Virus Infections Studies
- T-cell and B-cell Immunology
Amedes Genetics (Germany)
2021-2025
Technische Universität Braunschweig
2017-2022
The recent emergence of the Omicron variant has raised concerns on vaccine efficacy and urgent need to study more efficient vaccination strategies. Here we observed that an mRNA booster in individuals vaccinated with two doses inactivated significantly increased plasma level specific antibodies bind receptor-binding domain (RBD) or spike (S) ectodomain (S1 + S2) both G614 variants, compared homologous vaccine. RBD- S-specific IgG virus neutralization titers against variants concern...
The COVID-19 pandemic is caused by the betacoronavirus SARS-CoV-2. In November 2021, Omicron variant was discovered and immediately classified as a of concern (VOC), since it shows substantially more mutations in spike protein than any previous variant, especially receptor-binding domain (RBD). We analyzed binding RBD to human angiotensin-converting enzyme-2 receptor (ACE2) ability sera from patients or vaccinees comparison Wuhan, Beta, Delta variants.
The novel betacoronavirus severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) causes a form of pneumonia disease called coronavirus 2019 (COVID-19). To develop human neutralizing anti-SARS-CoV-2 antibodies, antibody gene libraries from convalescent COVID-19 patients were constructed and recombinant fragments (scFv) against the receptor-binding domain (RBD) spike protein selected by phage display. STE90-C11 shows subnanometer IC
Abstract COVID-19 is a severe acute respiratory disease caused by SARS-CoV-2, new recently emerged sarbecovirus. This virus uses the human ACE2 enzyme as receptor for cell entry, recognizing it with binding domain (RBD) of S1 subunit viral spike protein. We present use phage display to select anti-SARS-CoV-2 antibodies from naïve antibody gene libraries HAL9/10 and subsequent identification 309 unique fully against S1. 17 are RBD, showing inhibition cells expressing scFv-Fc neutralize active...
Abstract Diphtheria is an infectious disease caused by Corynebacterium diphtheriae . The bacterium primarily infects the throat and upper airways produced diphtheria toxin (DT), which binds to elongation factor 2 blocks protein synthesis, can spread through bloodstream affect organs, such as heart kidneys. For more than 125 years, therapy against has been based on polyclonal horse sera directed DT (diphtheria antitoxin; DAT). Animal have many disadvantages including serum sickness,...
Abstract Antibodies are essential tools for therapy and diagnostics. Yet, production remains expensive as it is mostly done in mammalian expression systems. As most therapeutic IgG require glycosylation to interact with the human immune system, other systems rarely used production. However, neutralizing antibodies that not required activate system well diagnostics, a cheaper would be advantageous. In our study, we show cost-efficient, easy high yield of various secreted antigens including...
Abstract Background There has been an unprecedented global effort to produce safe and effective vaccines against SARS-CoV-2. However, production challenges, supply shortages unequal reach, together with increased number of breakthrough infections due waning immunity the emergence new variants concern (VOC), have prolonged pandemic. To boost immune response, several heterologous vaccination regimes tested shown antibody responses compared homologous vaccination. Here we evaluated effect mRNA...
On July 2, 2024, the International Alliance for Biological Standardization (IABS) and Humane Society (HSI) co-hosted a webinar on global availability affordability of critical reagents vaccine biologics production. Despite growing support non-animal testing, significant barriers remain, especially in low-income countries facing financial supply chain challenges. This meeting showcased successful collaborations reagent production shared industry regulatory perspectives. Key included high...
Abstract The COVID-19 pandemic is caused by the betacoronavirus SARS-CoV-2. In November 2021, Omicron variant was discovered and classified as a of concern (VOC). shows substantially more mutations in spike protein than any previous variant, mostly receptor binding domain (RBD). We analyzed RBD to human ACE2 (hACE2) ability sera from patients or vaccinees comparison Wuhan, Beta Delta RBDs variants. All were produced insect cells. hACE2 ELISA microscale thermophoresis (MST). Similarly, 27...
Virus-like particles (VLPs) resemble authentic virus while not containing any genomic information. Here, we present a fast and powerful method for the production of SARS-CoV-2 VLP in insect cells application these VLPs to evaluate inhibition capacity monoclonal antibodies sera vaccinated donors. Our avoids baculovirus-based approaches commonly used by employing direct plasmid transfection co-express envelope, membrane, spike protein that self-assemble into VLPs. After optimization expression...
Abstract COVID-19 is a severe acute respiratory disease caused by SARS-CoV-2, novel betacoronavirus discovered in December 2019 and closely related to the SARS coronavirus (CoV). Both viruses use human ACE2 receptor for cell entry, recognizing it with Receptor Binding Domain (RBD) of S1 subunit viral spike (S) protein. The S2 domain mediates fusion host membrane. Experience MERS coronaviruses has shown that potent monoclonal neutralizing antibodies against RBD can inhibit interaction virus...
Abstract The novel betacoranavirus SARS-CoV-2 causes a form of severe pneumonia disease, termed COVID-19 (coronavirus disease 2019). Recombinant human antibodies are proven potent neutralizers viruses and can block the interaction viral surface proteins with their host receptors. To develop neutralizing anti-SARS-CoV-2 antibodies, antibody gene libraries from convalescent patients were constructed recombinant fragments (scFv) against receptor binding domain (RBD) S1 subunit spike (S) protein...
The novel betacoranavirus SARS-CoV-2 causes a form of severe pneumonia disease, termed COVID-19 (coronavirus disease 2019). Recombinant human antibodies are proven potent neutralizers viruses and can block the interaction viral surface proteins with their host receptors. To develop neutralizing anti-SARS-CoV-2 antibodies, antibody gene libraries from convalescent patients were constructed recombinant fragments (scFv) against receptor binding domain (RBD) S1 subunit spike (S) protein selected...
Members of the genus Aromatoleum thrive in diverse habitats and use a broad range recalcitrant organic molecules coupled to denitrification or O2 respiration. To gain holistic understanding model organism A. aromaticum EbN1T, we studied its catabolic network dynamics response 3-(4-hydroxyphenyl)propanoate, phenylalanine, 3-hydroxybenzoate, benzoate, acetate utilized under nitrate-reducing versus oxic conditions. Integrated multi-omics (transcriptome, proteome, metabolome) covered most (199...
Generation of sequence defined antibodies from universal libraries by phage display has been established over the past three decades as a robust method to cope with increasing market demand in therapy, diagnostics and research. For applications requiring bivalent antigen binding an Fc part for detection, generated single chain Fv (scFv) antibody fragments can rapidly be genetically fused moiety IgG production eukaryotic cells IgG-like properties. In contrast conversion scFv into format,...
Abstract One of the most widely used epitope tags is myc-tag, recognized by anti-c-Myc hybridoma antibody Myc1-9E10. Combining error-prone PCR, DNA shuffling and phage display, we generated an variant (Hyper-Myc) with monovalent affinity improved to 18 nM thermal stability increased 37%. Quantification capillary immunoblots flow cytometry demonstrated antigen detection Hyper-Myc. Further, three different species variants this were allow use either anti-human, anti-mouse or anti-rabbit Fc...
Today, recombinant antibodies can replace animal-derived primary in almost all applications. Due to their monoclonal origin and always known sequence, they offer optimal reproducibility. In contrast, secondary are still made from animal sera. Multiclonal by animal-free methods here a higher quality replacement for serum-derived antibodies.
To control the increasing virus pandemics, detection methods are essential. Today’s standard detections fast (immune assays) or precise (PCR). A method that is both and would enable more efficient mitigation measures better life comfort. According to recent papers, electrochemical impedance spectroscopy (EIS) has proven detect viruses precise. Boron-doped diamond (BDD) was used as a high-performance electrode material in these works. The aim of this work perform an initial test BDD-based EIS...
When preparing biological reference materials, the stability of lyophilized product is critical for long-term storage, particularly in order to meet WHO International Standards, which are not assigned expiry dates but expected be use several decades. Glass ampoules typically used by National Institute Biological Standards and Control (NIBSC) lyophilization materials. More recently, a clear need has arisen filling smaller volumes, may optimal. We investigated plastic microtubes as an...