A5010046875- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Salivary Gland Tumors Diagnosis and Treatment
- Head and Neck Cancer Studies
- AI in cancer detection
- Advanced Biosensing Techniques and Applications
- Photoacoustic and Ultrasonic Imaging
- Sinusitis and nasal conditions
- Remote-Sensing Image Classification
- Cancer Cells and Metastasis
- Image Processing Techniques and Applications
- Optical Imaging and Spectroscopy Techniques
- Cancer Genomics and Diagnostics
- SARS-CoV-2 and COVID-19 Research
- Brain Tumor Detection and Classification
- Ear and Head Tumors
- Head and Neck Surgical Oncology
- Lung Cancer Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Advanced Fluorescence Microscopy Techniques
- Photodynamic Therapy Research Studies
- Digital Imaging for Blood Diseases
- Oral and Maxillofacial Pathology
- Spectroscopy Techniques in Biomedical and Chemical Research
- Bone Tumor Diagnosis and Treatments
Klinikum Braunschweig
2015-2022
University of Bonn
2003-2014
Klinik und Poliklinik für Hals-Nasen-Ohren-Heilkunde
2004-2013
University Hospital Bonn
2008-2013
Klinik und Poliklinik für Hals-, Nasen-, Ohrenheilkunde
2004-2012
Zero to Three
2012
Klinik und Poliklinik für Hals-, Nasen-, Ohrenheilkunde
2011
Universitäts-HNO-Klinik Heidelberg
2011
Leipzig University
2000-2003
The novel betacoronavirus severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) causes a form of pneumonia disease called coronavirus 2019 (COVID-19). To develop human neutralizing anti-SARS-CoV-2 antibodies, antibody gene libraries from convalescent COVID-19 patients were constructed and recombinant fragments (scFv) against the receptor-binding domain (RBD) spike protein selected by phage display. STE90-C11 shows subnanometer IC
Hyperspectral imaging (HSI) is increasingly gaining acceptance in the medical field. Up until now, HSI has been used conjunction with rigid endoscopy to detect cancer vivo. The logical next step pair flexible endoscopy, since it improves access hard-to-reach areas. While endoscope's fiber optic cables provide advantage of flexibility, they also introduce an interfering honeycomb-like pattern onto images. Due substantial impact this on locating cancerous tissue, must be removed before HS data...
The coronavirus SARS-CoV-2 is the causative agent for disease COVID-19. To capture IgA, IgG, and IgM antibody response of patients infected with at individual epitope resolution, we constructed planar microarrays 648 overlapping peptides that cover four major structural proteins S(pike), N(ucleocapsid), M(embrane), E(nvelope). arrays were incubated sera 67 positive 22 negative control samples. Specific responses to detectable, nine associated a more severe course disease. A random forest...
Natural killer (NK) and NK T (NKT) cells are important in innate immune defense. Their unequivocal identification requires at least four antigens. Based on the expression of additional antigens, they can be further divided into functional subsets. For more accurate immunophenotyping to describe multiple patterns leukocyte subsets, an increased number measurable colors is necessary. To take advantage technologic features offered by slide-based cytometry, repeated analysis was combined with...
Hyperspectral imaging (HSI) is a technology with high potential in the field of non-invasive detection cancer. However, complex situations like HSI larynx rigid endoscope, various image interferences can disable proper classification cancerous tissue. We identified three main problems: i) misregistration single images HS cube due to patient heartbeat ii) noise and iii) specular reflections (SR). Consequently, an pre-processor developed current paper overcome these interferences. It...
Currently, there are no fast and accurate screening methods available for head neck cancer, the eighth most common tumor entity. For this study, we used hyperspectral imaging, an imaging technique quantitative objective surface analysis, combined with deep learning automated tissue classification. As part of a prospective clinical observational datasets laryngeal, hypopharyngeal oropharyngeal mucosa were recorded in 98 patients before surgery vivo. We established data interpretation pathway...
Abstract Aim Slide‐based cytometry (SBC) allows to “ask a cell second time.” We used this tool for detailed immunophenotyping of peripheral blood leukocytes (PBLs). Methods PBLs primarily stained CD‐markers and DNA were immobilized on glass slide analyzed by laser scanning cytometry. Then, iterative restaining was applied third analysis. Based the cells' fixed location, analyses merged single‐cell level. Results six virtual immunostainings “recycling” PE‐channel four CD‐markers. Conclusion...
Abstract The aim of this study was to proof applicability hyperspectral imaging for the analysis and classification human mucosal surfaces in vivo. larynx as a prototypical anatomically well‐defined surgical test area analyzed by microlaryngoscopy with polychromatic lightsource synchronous triggered monochromatic CCD‐camera. Image stacks (5 benign, 7 malignant tumors) were established software (principal component PCA, classification, spectral profiles). Hyperspectral image datacubes...
In the field of earth observation, hyperspectral detector systems allow precise target detections surface components from remote sensing platforms. This enables specific land covers to be identified without need physically travel areas examined. medical field, efforts are underway develop optical technologies that detect altering tissue surfaces necessity perform an excisional biopsy. With establishment expedient classification procedures, imaging may provide a non-invasive diagnostic method...
The immune checkpoint, indoleamine 2,3-dioxygenase 1, is under investigation as target of novel immunotherapies for cancers, including head and neck squamous cell carcinomas (HNSCC). aim our study was to analyze DNA methylation the encoding gene (IDO1) in HNSCC.Methylation three CpG sites within promoter, promoter flank, body investigated correlated with mRNA expression, infiltration, mutational burden, human papillomavirus (HPV)-status, overall survival a cohort N = 528 HNSCC patients...
In lymphatic organs, the quantitative analysis of spatial distribution leukocytes by tissue cytometry would give relevant information about alterations during diseases (leukemia, HIV, AIDS) and their therapeutic regimen, as well in experimental settings.We have developed a semiautomated method for laser scanning (LSC) termed "multiple thresholding," which is suitable archived or fresh biopsy material human lymph nodes tonsils. Sections are stained with PI nuclear DNA up to four antigens...
To adequately analyze the complexity of immune system and reduce required sample volume for immunophenotyping in general, more measurable colors discrimination leukocyte subsets are necessary. Immunophenotyping by laser scanning cytometer (LSC), a slide-based cytometric technology, combines cell detection based on multiple with their subsequent visualization without need physical sorting. In present study, filter setting LSC was adapted measurement far-red emitting dye cyanine 7 (Cy7),...