A5035588954- Physics and Engineering Research Articles
- Renewable Energy and Sustainability
- Cellular Mechanics and Interactions
- Monoclonal and Polyclonal Antibodies Research
- 3D Printing in Biomedical Research
- Theoretical and Computational Physics
- SARS-CoV-2 and COVID-19 Research
- Fluid Dynamics and Thin Films
- Photosynthetic Processes and Mechanisms
- nanoparticles nucleation surface interactions
- Bacteriophages and microbial interactions
- Copper Interconnects and Reliability
- Spectroscopy and Quantum Chemical Studies
- Magnetic properties of thin films
- Electrospun Nanofibers in Biomedical Applications
- Surface Modification and Superhydrophobicity
- Bone Tissue Engineering Materials
- X-ray Diffraction in Crystallography
- Mechanical and Optical Resonators
- Protein purification and stability
- Digital Innovation in Industries
- Carbohydrate Chemistry and Synthesis
- Microfluidic and Bio-sensing Technologies
- CAR-T cell therapy research
- Glycosylation and Glycoproteins Research
University Medical Center Groningen
2014-2023
University of Groningen
2014-2023
Saarland University
2022
Technische Universität Braunschweig
2016-2017
Deutsches Zentrum für Luft- und Raumfahrt e. V. (DLR)
2013-2015
Institute for Biomedical Engineering
2014
Heinrich Heine University Düsseldorf
2012
University of Cologne
2005-2008
Technische Universität Berlin
2005-2006
University of Duisburg-Essen
2002-2005
The novel betacoronavirus severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) causes a form of pneumonia disease called coronavirus 2019 (COVID-19). To develop human neutralizing anti-SARS-CoV-2 antibodies, antibody gene libraries from convalescent COVID-19 patients were constructed and recombinant fragments (scFv) against the receptor-binding domain (RBD) spike protein selected by phage display. STE90-C11 shows subnanometer IC
Abstract COVID-19 is a severe acute respiratory disease caused by SARS-CoV-2, new recently emerged sarbecovirus. This virus uses the human ACE2 enzyme as receptor for cell entry, recognizing it with binding domain (RBD) of S1 subunit viral spike protein. We present use phage display to select anti-SARS-CoV-2 antibodies from naïve antibody gene libraries HAL9/10 and subsequent identification 309 unique fully against S1. 17 are RBD, showing inhibition cells expressing scFv-Fc neutralize active...
High-throughput screening (HTS) methods based on topography gradients or arrays have been extensively used to investigate cell–material interactions. However, it is a huge technological challenge cost efficiently prepare topographical of inorganic biomaterials due their inherent material properties. Here, we developed novel strategy translating PDMS-based wrinkled with amplitudes from 49 2561 nm and wavelengths between 464 7121 (SiO2, Ti/TiO2, Cr/CrO3, Al2O3) which are frequently clinical...
Abstract A novel approach was developed using PDMS-substrates with surface-aligned nanotopography gradients, varying unidirectional in amplitude and wavelength, for studying cell behavior regard to adhesion alignment. The gradients target more surface feature parameters simultaneously provide information fewer experiments are therefore vastly superior respect individual topography substrates. Cellular on non-gradient aligned nanowrinkled surfaces displayed a linear relationship of osteoblast...
In this review, current research in the field of biomaterial properties for directing stem cells are discussed and placed a critical perspective.
We discuss the dependence of self- and interdiffusion coefficients on temperature composition for two prototypical binary metallic melts, Al-Ni Zr-Ni, in molecular-dynamics (MD) computer simulations mode-coupling theory glass transition (MCT). Dynamical processes that are mainly entropic origin slow down mass transport (as expressed through self diffusion) mixture as compared to ideal-mixing contribution. Interdiffusion chemical species is a competition kinetic modes with strong...
Advancing biomedicine by tunable e-transfer into/from microgels.
The shape evolution of two-dimensional islands through periphery diffusion biased by an electromigration force is studied numerically using a continuum approach. We show that the introduction crystal anisotropy in mobility edge atoms induces rich variety migration modes, which include oscillatory and irregular behavior. A phase diagram plane strength island size constructed. motion can be understood terms stable facets develop on one side island, then slides past. facet orientations are...
Progenitor cells cultured on biomaterials with optimal physical-topographical properties respond alignment and differentiation. Stromal from connective tissue can adversely differentiate to profibrotic myofibroblasts or favorably smooth muscle (SMC). We hypothesized that myogenic differentiation of adipose tissue-derived stromal (ASC) depends gradient directional topographic features. Polydimethylsiloxane (PDMS) samples nanometer micrometer topography gradients (wavelength (w) = 464-10, 990...
Abstract Surface gradients provide a powerful platform to accelerate multiscale design by efficiently studying of material–cell interactions ultimately enhance function synthetic clinical biomaterials. Herein, novel orthogonal double gradient is reported in which surface stiffness and wettability vary independently continuously perpendicular directions, providing unique combinations over broad range (stiffness: 6–89 MPa; water contact angle: 29°–90°). It found that mesenchymal stem cell...
Tailoring cell–surface interactions is important for the of design medical implants as well regenerative medicine and tissue engineering materials. Here single parameter system transcended via translating hard nanotopology into soft polymeric hydrogel structures imprinting lithography. The response these cells to same dimensions but with different mechanical properties displays unexpected behavior between "hard" "soft" cells. As a service our authors readers, this journal provides supporting...
Abstract N-methyl-D-aspartate receptor (NMDAR) encephalitis is the most common subtype of autoimmune characterized by a complex neuropsychiatric syndrome usually including memory impairment. Patients develop an intrathecal immune response against NMDARs with antibodies that presumably bind to amino-terminal domain GluN1 subunit. The therapeutic immunotherapy often delayed. Therefore, new approaches for fast neutralization NMDAR are needed. Here, we developed fusion constructs consisting Fc...
The multiphasic P680+˙ reduction kinetics by YZ and their temperature dependence were investigated in PS II core complexes with high oxygen evolution capacity, isolated from a thermophilic cyanobacterium (Thermosynechococcus elongatus) higher plant (Spinacea oleracea). Measurements kinetic analyses of laser flash induced 820 nm absorption changes (reflecting the turnover P680) led to following results: (a) pattern is basically same both species, (b) activation energy "fast" nanosecond 20 ± 5...
The shape evolution of two-dimensional islands under electromigration-driven periphery diffusion is studied by kinetic Monte Carlo (KMC) simulations and continuum theory. energetics the KMC model adapted to Cu(100) surface, matched a suitably parametrized choice orientation-dependent step stiffness atom mobility. At $700\phantom{\rule{0.3em}{0ex}}\mathrm{K}$ oscillations predicted theory are quantitatively verified simulations, while at $500\phantom{\rule{0.3em}{0ex}}\mathrm{K}$ qualitative...
Abstract Two‐parameter contributions of stiffness and wettability display altered behavior hBM‐MSCs as compared to solely investigating the biointerface with a homogenous stiffness. The importance double parameter influence is identified by using newly developed linear wettability–stiffness gradients. interfaces show that substrate can cell adhesion spreading both positively negatively in combination wettability. This effect validated decoupling gradient composed monolayer on glass ensuring
Biopolymers are an attractive class of compounds for being used in biomedical applications as they widely available from biomass. Their drawback is the lack mechanical stability and ability to tune this properly. Covalent chemical cross-linking often approach but it limits usability due legislation well need advanced specialized knowledge by end users such clinicians. Here, increased tunable properties achieved alginate-based hydrogels with non-covalent approaches using linear...
Abstract COVID-19 is a severe acute respiratory disease caused by SARS-CoV-2, novel betacoronavirus discovered in December 2019 and closely related to the SARS coronavirus (CoV). Both viruses use human ACE2 receptor for cell entry, recognizing it with Receptor Binding Domain (RBD) of S1 subunit viral spike (S) protein. The S2 domain mediates fusion host membrane. Experience MERS coronaviruses has shown that potent monoclonal neutralizing antibodies against RBD can inhibit interaction virus...