A5020750258- Cytomegalovirus and herpesvirus research
- Neuroinflammation and Neurodegeneration Mechanisms
- COVID-19 Clinical Research Studies
- Herpesvirus Infections and Treatments
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- PARP inhibition in cancer therapy
- Immune Response and Inflammation
- Tryptophan and brain disorders
- Cannabis and Cannabinoid Research
- Long-Term Effects of COVID-19
- Diet, Metabolism, and Disease
- Advanced Breast Cancer Therapies
- Intensive Care Unit Cognitive Disorders
- Virus-based gene therapy research
- COVID-19 diagnosis using AI
- SARS-CoV-2 detection and testing
- Histone Deacetylase Inhibitors Research
- Toxoplasma gondii Research Studies
- Adipokines, Inflammation, and Metabolic Diseases
- Neonatal Health and Biochemistry
- Immune cells in cancer
- Mental Health Research Topics
- Viral-associated cancers and disorders
- Bacillus and Francisella bacterial research
University of Rijeka
2021-2025
University of Ljubljana
2020-2022
National Institute of Biology
2022
The COVID-19 pandemic is caused by the betacoronavirus SARS-CoV-2. In November 2021, Omicron variant was discovered and immediately classified as a of concern (VOC), since it shows substantially more mutations in spike protein than any previous variant, especially receptor-binding domain (RBD). We analyzed binding RBD to human angiotensin-converting enzyme-2 receptor (ACE2) ability sera from patients or vaccinees comparison Wuhan, Beta, Delta variants.
Congenital human cytomegalovirus (cHCMV) infection of the brain is associated with a wide range neurocognitive sequelae. Using newborn mice mouse (MCMV) as reliable model that recapitulates many aspects cHCMV infection, including disseminated CNS altered neurodevelopment, and sensorineural hearing loss, we have previously shown mitigation inflammation prevented alterations in cerebellar development, suggesting host inflammatory factors are key drivers neurodevelopmental defects. Here, show...
Abstract To date, no herpesvirus has been shown to latently persist in fibroblastic cells. Here, we show that murine cytomegalovirus, a β-herpesvirus, persists for the long term and across organs PDGFRα-positive cells, with similar or higher genome loads than previously known sites of cytomegalovirus latency. Whereas gene transcription cells is almost completely silenced at 5 months post-infection, these give rise reactivated virus ex vivo, arguing they support latent infection. Notably,...
The aim of this study was to characterize the systemic cytokine signature critically ill COVID-19 patients in a high mortality setting aiming identify biomarkers severity, and explore their associations with viral loads clinical characteristics. We studied two patient cohorts from referral centre located Central Europe. were recruited during pre-alpha/alpha (November 2020 April 2021) delta (end period respectively. determined both serum bronchoalveolar SARS-CoV-2 load identified variant...
All human herpesviruses establish latency following the resolution of primary infection. Among these, α-herpesviruses HSV-1, HSV-2 and VZV in neurons, whereas neurons are not traditionally considered a site for other herpesviruses. Using combination vivo murine models ex fetal tissues, we discovered that cytomegalovirus (CMV), ubiquitous β-herpesvirus, can persist CD4+ T-cell-derived interferon-gamma is critical restricting active viral replication this cell type. Furthermore, show mouse CMV...
Abstract The COVID-19 pandemic is caused by the betacoronavirus SARS-CoV-2. In November 2021, Omicron variant was discovered and classified as a of concern (VOC). shows substantially more mutations in spike protein than any previous variant, mostly receptor binding domain (RBD). We analyzed RBD to human ACE2 (hACE2) ability sera from patients or vaccinees comparison Wuhan, Beta Delta RBDs variants. All were produced insect cells. hACE2 ELISA microscale thermophoresis (MST). Similarly, 27...
This article describes procedures for infecting adult mice with murine cytomegalovirus (MCMV) and newborn to model congenital CMV infection. Methods are included propagating MCMV in cell cultures preparing a more virulent form of from the salivary glands infected mice. A plaque assay is provided determining titers tissues or virus stocks. Also, methods described embryonic fibroblasts used MCMV, assay. © 2022 Wiley Periodicals LLC.
While SARS-CoV-2 detection in sputum and swabs from the upper respiratory tract has been used as a diagnostic tool, virus quantification showed poor correlation to disease outcome thus, prognostic value. Although pulmonary compartment represents relevant site for viral load analysis, limited data exploring lower is available, its association clinical outcomes relatively unknown. Using bronchoalveolar lavage (BAL) serum samples, we quantified copy numbers systemic compartments of critically...
Studies assessing the dynamics and duration of antibody responses following SARS-CoV-2 infection or vaccination are an invaluable tool for schedule planning, assessment risk groups management pandemics. In this study, we developed employed ELISA assays to analyze humoral Nucleocapsid Spike proteins in vaccinated health-care workers (HCW) critically ill COVID-19 patients. Sera more than 1000 HCWs patients from Clinical Hospital Center Rijeka were tested across a one-year period, encompassing...
<title>Abstract</title> Microglia are myeloid cells that reside within the central nervous system (CNS), where they maintain homeostasis under normal, non-pathological conditions. In addition, microglia also perform numerous immune functions upon different pathogenic stimuli, including CNS infections with various neurotropic viruses. Herpesviruses establish a lifelong latent infection from which reactivate intermittently waning of control. The role in preventing reactivation herpesviruses...
Breast cancer is the most common malignancy in women worldwide. Sixty-five percent of breast cancers are estrogen and/or progesterone receptor positive. Estrogen expression a prognostic and predictive biomarker response to endocrine therapy, which consists selective modulator tamoxifen, aromatase inhibitors, degrader fulvestrant. Cannabidiol phytocannabinoid that emerging as potential therapeutic agent. The aim this study was investigate effect cannabidiol on receptor-positive...
<title>Abstract</title> Human cytomegalovirus (HCMV) is the leading cause of congenital viral infections, frequently accompained with long-term neurological sequelae in children. The cell types and mechanisms involved establishing lifelong CMV latency brain, from which virus reactivates intermittently, remain enigmatic. Infection newborn mice mouse (MCMV) closely mimicks pathophysiology HCMV was used to unravel factors infection central nervous system (CNS). Here we show that cortex...
Human cytomegalovirus (HCMV) rarely infects the brain following infection of adult individuals. However, virus readily during congenital HCMV (cHCMV) infection, frequently causing severe neurodevelopmental and neurological sequelae. Interestingly, although incidence cHCMV is 0.5–1%, proportion congenitally infected individuals in which manages to gain access unknown. In this study, we used mice with mouse (MCMV), most commonly experimental system for modeling disease humans, determine impact...
Abstract To date, no herpesvirus has been shown to latently persist in fibroblastic cells. Here, we demonstrate that mouse CMV (MCMV), a β-herpesvirus, persists for the long term and across organs PDGFRα + cells, with similar or higher genome loads than previously known sites of MCMV latency. Whereas gene transcription cells was almost completely silenced at 5 months post-infection, these gave rise reactivated virus ex vivo , arguing they supported latent infection. Notably, also productive...
Abstract Background: Breast cancer (BC) is a very heterogeneous disease. Currently, decision-making regarding treatment of individual patients based on several factors, most important them are expression estrogen receptors (ER), progesterone and receptor for epidermal growth factor 2 (HER2). Common endocrine therapies include selective modulator tamoxifen (TAM), aromatase inhibitors (AIs) degrader fulvestrant. Inhibitors cyclin dependent kinases 4/6 (iCDK 4/6) in combination with AIs or...
Breast cancer is the most common malignancy in women worldwide. Sixty-five percent of breast cancers are estrogen and/or progesterone receptor positive. Estrogen expression a prognostic and predictive biomarker response to endocrine therapy, which consists selective modulator tamoxifen, aromatase inhibitors, degrader fulvestrant. Cannabidiol phytocannabinoid that emerging as potential therapeutic agent. The aim this study was investigate effect cannabidiol on receptor-positive...