A5005814127- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- SARS-CoV-2 and COVID-19 Research
- Insect symbiosis and bacterial influences
- Long-Term Effects of COVID-19
- COVID-19 Clinical Research Studies
- Viral gastroenteritis research and epidemiology
- Viral Infections and Vectors
- Education, Psychology, and Social Research
- Psychology of Development and Education
- Economic Issues in Ukraine
- Biochemical and Molecular Research
- Resilience and Mental Health
- Adventure Sports and Sensation Seeking
- Bipolar Disorder and Treatment
- Medical and Pharmaceutic Studies
- Influenza Virus Research Studies
- Animal Virus Infections Studies
- Phytoplasmas and Hemiptera pathogens
Università della Svizzera italiana
2022-2023
University of Ostrava
2016-2021
Abstract Infection with severe acute respiratory syndrome coronavirus 2 associates diverse symptoms, which can persist for months. While antiviral antibodies are protective, those targeting interferons and other immune factors associated adverse disease 2019 (COVID-19) outcomes. Here we discovered that against specific chemokines were omnipresent post-COVID-19, favorable outcome negatively correlated the development of long COVID at 1 yr post-infection. Chemokine also present in HIV-1...
Emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants diminishes the efficacy vaccines and antiviral monoclonal antibodies. Continued development immunotherapies vaccine immunogens resilient to viral evolution is therefore necessary. Using coldspot-guided antibody discovery, a screening approach that focuses on portions virus spike glycoprotein are both functionally relevant averse change, we identified human neutralizing antibodies highly conserved epitopes....
Trypanosomatids of the genus Leishmania are parasites mammals or reptiles transmitted by bloodsucking dipterans. Many species these flagellates cause important human diseases with clinical symptoms ranging from skin sores to life-threatening damage visceral organs. The contains four subgenera: Leishmania, Sauroleishmania, Viannia, and Mundinia. last subgenus has been established recently remains understudied, although Mundinia human-infecting species. In addition, it is interesting...
Leishmaniases are neglected tropical diseases and Leishmania (Leishmania) infantum (Viannia) braziliensis the most important causative agents of leishmaniases in New World. These two parasite species may co-circulate a given endemic area but their interactions vector have not been studied yet. We conducted experimental infections using both single co-infections to compare development L. (L.) (OGVL/mCherry) (V.) (XB29/GFP) Lutzomyia longipalpis migonei. Parasite labelling by different...
Leishmania virulence factors responsible for the complicated epidemiology of various leishmaniases remain mainly unidentified. This study is a characterization gene previously identified as upregulated in two three overlapping datasets containing putative important Leishmania's ability to establish mammalian intracellular infection and colonize gut an insect vector.The investigated encodes ATP/GTP binding motif-containing protein related development 1 (ALD1), cytosolic that contains cryptic...
In our previous work we established a T7 polymerase-driven Tetracycline-inducible protein expression system in Leishmania mexicana (Biagi, 1953).We used this to analyse gene profiles during development of L. procyclic and metacyclic promastigotes amastigotes.The transcription the interest polymerase genes was significantly reduced upon cell differentiation.This regulation is not locus-specific.It depends on untranslated regions flanking open reading frames analysed.In paper, report that...
Catalase is one of the most abundant enzymes on Earth. It decomposes hydrogen peroxide, thus protecting cells from dangerous reactive oxygen species. The catalase-encoding gene conspicuously absent genome representatives family Trypanosomatidae. Here, we expressed this protein Leishmania mexicana Β-TUBULIN locus using a novel bicistronic expression system, which relies 2A peptide Teschovirus A. We demonstrated that catalase-expressing parasites are severely compromised in their ability to...
Leishmania parasites cause a variety of symptoms, including mucocutaneous leishmaniasis, which results in the destruction mucous membranes nose, mouth, and throat. The species carrying RNA virus 1 (LRV1), from family Totiviridae, are more likely to severe disease less sensitive treatment than those that do not contain virus. Although importance LRV1 for severity leishmaniasis was discovered long time ago, structure remained unknown. Here, we present cryo-electron microscopy reconstruction...
The closest relative of human pathogen Leishmania, the trypanosomatid Novymonas esmeraldas, harbors a bacterial endosymbiont "Candidatus Pandoraea novymonadis." Based on genomic data, we performed detailed characterization metabolic interactions both partners. While in many respects metabolism N. esmeraldas resembles that other Leishmaniinae, provides with heme, essential amino acids, purines, some coenzymes, and vitamins. In return, shares bacterium several nonessential acids phospholipids....
Leishmania are obligate intracellular parasites known to have developed successful ways of efficient immunity evasion. Because this, leishmaniasis, a disease caused by these flagellated protists, is ranked as one the most serious tropical infections worldwide. Neither prophylactic medication, nor vaccination has been thus far, even though infection usually led strong and long-lasting immunity. In this paper, we describe "suicidal" system established in mexicana, human pathogen causing...
Abstract Infection by SARS-CoV-2 leads to diverse symptoms, which can persist for months. While antiviral antibodies are protective, those targeting interferons and other immune factors associated with adverse COVID-19 outcomes. Instead, we discovered that against specific chemokines omnipresent after COVID-19, favorable disease, predictive of lack long COVID symptoms at one year post infection. Anti-chemokine present also in HIV-1 infection autoimmune disorders, but they target different...
Emergence of SARS-CoV-2 variants diminishes the efficacy vaccines and antiviral monoclonal antibodies. Continued development immunotherapies vaccine immunogens resilient to viral evolution is therefore necessary. Using coldspot-guided antibody discovery, a screening approach that focuses on portions virus spike are both functionally relevant averse change, we identified human neutralizing antibodies highly conserved epitopes. Antibody fp.006 binds fusion peptide cross-reacts against...