A5091666023- Genomics and Phylogenetic Studies
- Protist diversity and phylogeny
- Parasitic Infections and Diagnostics
- Microbial Community Ecology and Physiology
- Plant nutrient uptake and metabolism
- Trypanosoma species research and implications
- Insect symbiosis and bacterial influences
- RNA and protein synthesis mechanisms
- Photosynthetic Processes and Mechanisms
- RNA modifications and cancer
- Plant Disease Resistance and Genetics
- Myxozoan Parasites in Aquatic Species
- RNA Research and Splicing
- Methane Hydrates and Related Phenomena
- Cancer-related molecular mechanisms research
- Legume Nitrogen Fixing Symbiosis
- Toxoplasma gondii Research Studies
- Molecular Biology Techniques and Applications
- Plant-Microbe Interactions and Immunity
- Monoclonal and Polyclonal Antibodies Research
- Viral Infections and Immunology Research
- Research on Leishmaniasis Studies
- Advanced Proteomics Techniques and Applications
- Marine Ecology and Invasive Species
- Phytoplasmas and Hemiptera pathogens
Institute of Parasitology
2017-2024
Czech Academy of Sciences, Biology Centre
2017-2024
University of Bristol
2023
University of South Bohemia in České Budějovice
2017-2023
Czech Academy of Sciences
2017-2019
Photosynthetic euglenids are major contributors to fresh water ecosystems. Euglena gracilis in particular has noted metabolic flexibility, reflected by an ability thrive a range of harsh environments. E. been popular model organism and considerable biotechnological interest, but the absence gene catalogue hampered both basic research translational efforts. We report detailed transcriptome partial genome for Z1. The nuclear is estimated be around 500 Mb size, encodes over 36,000 proteins...
Abstract Background Diplonemid flagellates are among the most abundant and species-rich of known marine microeukaryotes, colonizing all habitats, depths, geographic regions world ocean. However, little is about their genomes, biology, ecological role. Results We present first nuclear genome sequence from a diplonemid, type species Diplonema papillatum . The ~ 280-Mb assembly contains 32,000 protein-coding genes, likely co-transcribed in groups up to 100. Gene clusters separated by long...
Parasites tend to evolve small and compact genomes, generally endowed with a high mutation rate, compared those of their free-living relatives. However, the mechanisms by which they achieve these features, independently in unrelated lineages, remain largely unknown. We argue that loss classical nonhomologous end joining pathway components may be one crucial steps responsible for characteristic features parasite genomes.
Euglena gracilis is a metabolically flexible, photosynthetic, and adaptable free-living protist of considerable environmental importance biotechnological value. By label-free liquid chromatography tandem mass spectrometry, total 1,786 proteins were identified from the E. purified mitochondria, representing one largest mitochondrial proteomes so far described. Despite this apparent complexity, protein machinery responsible for extensive RNA editing, splicing, processing in sister clades...
We have sequenced, annotated, and analyzed the genome of Ca. Pandoraea novymonadis, a recently described bacterial endosymbiont trypanosomatid Novymonas esmeraldas. When compared with genomes its free-living relatives, it has all hallmarks endosymbionts' genomes, such as significantly reduced size, extensive gene loss, low GC content, numerous rearrangements, codon usage bias. In addition, P. novymonadis lacks mobile elements, strikingly number pseudogenes, almost genes are single copied....
Abstract Myxozoans (Cnidaria: Myxozoa) are an extremely diversified group of endoparasites some which causative agents serious diseases in fish. New methods involving gene expression studies have emerged over the last years to better understand and control myxozoan diseases. Quantitative RT-PCR is most extensively used approach for studies. However, accuracy results depends on normalization data reference genes. We studied eight commonly genes, adenosylhomocysteinase (AHC1), beta actin...
The closest relative of human pathogen Leishmania, the trypanosomatid Novymonas esmeraldas, harbors a bacterial endosymbiont "Candidatus Pandoraea novymonadis." Based on genomic data, we performed detailed characterization metabolic interactions both partners. While in many respects metabolism N. esmeraldas resembles that other Leishmaniinae, provides with heme, essential amino acids, purines, some coenzymes, and vitamins. In return, shares bacterium several nonessential acids phospholipids....
In kinetoplastid protists, all mitochondrial tRNAs are encoded in the nucleus and imported from cytoplasm to maintain organellar translation. This also applies tryptophanyl tRNA (tRNATrp) by a single-copy nuclear gene, with CCA anticodon read UGG codon used cytosolic Yet, mitochondrion it is unable decode UGA specifying tryptophan. Following import of tRNATrp, this problem solved at RNA level single C34 U34 editing event that creates UCA anticodon, recognizing UGA. To identify enzyme...
Abstract Photosynthetic euglenids are major components of aquatic ecosystems and relatives trypanosomes. Euglena gracilis has considerable biotechnological potential great adaptability, but exploitation remains hampered by the absence a comprehensive gene catalogue. We address this genome, RNA protein sequencing: E. genome is >2Gb, with 36,526 predicted proteins. Large lineage-specific paralog families present, evidence for flexibility in environmental monitoring, divergent mechanisms...