A5024443082- Trypanosoma species research and implications
- Plant Virus Research Studies
- CRISPR and Genetic Engineering
- DNA Repair Mechanisms
- DNA and Nucleic Acid Chemistry
- Biochemical and Molecular Research
- Acute Lymphoblastic Leukemia research
- Parasitic Infections and Diagnostics
- Research on Leishmaniasis Studies
- Insect symbiosis and bacterial influences
Wellcome Centre for Molecular Parasitology
2019-2025
University of Glasgow
2019-2025
Parasites tend to evolve small and compact genomes, generally endowed with a high mutation rate, compared those of their free-living relatives. However, the mechanisms by which they achieve these features, independently in unrelated lineages, remain largely unknown. We argue that loss classical nonhomologous end joining pathway components may be one crucial steps responsible for characteristic features parasite genomes.
RNA-DNA hybrids are epigenetic features of all genomes that intersect with many processes, including transcription, telomere homeostasis, and centromere function. Increasing evidence suggests can provide two conflicting roles in the maintenance transmission genomes: They be triggers DNA damage, leading to genome change, or aid repair processes needed respond lesions. Evasion host immunity by African trypanosomes, such as Trypanosoma brucei, relies on targeted recombination silent Variant...
The Trypanosoma brucei genome is structurally complex. Eleven megabase-sized chromosomes each comprise a transcribed core flanked by silent subtelomeres, housing thousands of Variant Surface Glycoprotein (VSG) genes. Additionally, hundreds sub-megabase contain 177 bp repeats unknown function, and VSG transcription sites localise to many telomeres. DNA replication dynamics have only been described in the megabase chromosome cores, single active site. Using Nanopore assembly, we show that...
Abstract Genomes in eukaryotes normally undergo DNA replication a choreographed temporal order, resulting early and late replicating chromosome compartments. Leishmania , human protozoan parasite, displays an unconventional program which the timing of completion is size-dependent: larger chromosomes complete later then smaller ones. Here we show that both R-loops RNase H1, ribonuclease resolves RNA-DNA hybrids, accumulate major pattern reflects their timing. Furthermore, demonstrate such...
Abstract The genome of Trypanosoma brucei is structurally complex. Eleven megabase-sized chromosomes each comprise a transcribed core flanked by silent subtelomeres, housing thousands Variant Surface Glycoprotein ( VSG ) genes. Additionally, VSGs are also found on hundreds sub-megabase that harbour 177 bp repeats unknown function, and multiple transcription sites localise to the telomeres both chromosome types. DNA replication dynamics have been described in megabase cores but not...
Abstract Genomes in eukaryotes normally undergo DNA replication a choreographed temporal order, resulting early and late replicating chromosome compartments. Leishmania , human protozoan parasite, displays an unconventional program which the timing of completion is size-dependent: larger chromosomes complete later then smaller ones. Here we show that both R-loops RNase H1, ribonuclease resolves RNA-DNA hybrids, accumulate major pattern reflects their timing. Furthermore, demonstrate such...
Abstract Understanding genome duplication requires characterisation of the locations where DNA replication initiates, termed origins. Genome-wide mapping origins has mainly been derived from population-based techniques, with only a few studies examining origin location and usage at single-cell or single-molecule level. Leishmania are protozoan parasites first attempt to map suggested unprecedented use, for eukaryote, just single per chromosome, while subsequent approach around 200-fold more...
Abstract RNA-DNA hybrids are epigenetic features of all genomes that intersect with many processes, including transcription, telomere homeostasis and centromere function. Increasing evidence suggests can provide two conflicting roles in the maintenance transmission genomes: they be triggers DNA damage, leading to genome change, or aid repair processes needed respond lesions. Evasion host immunity by African trypanosomes, such as Trypanosoma brucei , relies on targeted recombination silent...