A5103079026- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- Genomics and Chromatin Dynamics
- Pluripotent Stem Cells Research
- Cancer Genomics and Diagnostics
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- 3D Printing in Biomedical Research
- RNA modifications and cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Bioinformatics and Genomic Networks
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Genetic factors in colorectal cancer
- Gene expression and cancer classification
- CAR-T cell therapy research
- Biomedical Ethics and Regulation
- Gene Regulatory Network Analysis
- Immune cells in cancer
- Molecular Biology Techniques and Applications
- Neuroscience and Music Perception
- Cellular Mechanics and Interactions
- Chromatography in Natural Products
- Adipose Tissue and Metabolism
University of Seoul
2021-2024
Seoul National University
2011-2024
Seoul National University Hospital
2012-2024
Salk Institute for Biological Studies
2018-2023
QIMR Berghofer Medical Research Institute
2015
Konkuk University
2015
Wonkwang University
2014
National Research Institute of Cultural Heritage
2010
The role of individual subunits in the targeting and function mammalian BRG1-associated factors (BAF) complex embryonic stem cell (ESC) pluripotency maintenance has not yet been elucidated. Here we find that Bromodomain containing protein 9 (BRD9) Glioma tumor suppressor candidate region gene 1 (GLTSCR1) or its paralog GLTSCR1-like (GLTSCR1L) define a smaller, non-canonical BAF (GBAF complex) mouse ESCs is distinct from canonical ESC (esBAF). GBAF esBAF complexes are targeted to different...
Single-cell technologies measure unique cellular signatures but are typically limited to a single modality. Computational approaches allow the fusion of diverse single-cell data types, their efficacy is difficult validate in absence authentic multi-omic measurements. To comprehensively assess molecular phenotypes cells, we devised single-nucleus methylcytosine, chromatin accessibility, and transcriptome sequencing (snmCAT-seq) applied it postmortem human frontal cortex tissue. We developed...
Abstract Single-cell omics technologies have revolutionized molecular profiling by providing high-resolution insights into cellular heterogeneity and complexity. Traditional bulk approaches average signals from heterogeneous cell populations, thereby obscuring important nuances. studies enable the analysis of individual cells reveal diverse types, dynamic states, rare populations. These techniques offer unprecedented resolution sensitivity, enabling researchers to unravel landscape cells....
Abstract Reprogramming of somatic cells to induced pluripotent stem involves a dynamic rearrangement the epigenetic landscape. To characterize this epigenomic roadmap, we have performed MethylC-seq, ChIP-seq (H3K4/K27/K36me3) and RNA-Seq on samples taken at several time points during murine secondary reprogramming as part Project Grandiose. We find that DNA methylation gain occurs gradually, while loss is achieved only ESC-like state. Binding sites activated factors exhibit focal...
Throughout an individual's lifetime, genomic alterations accumulate in somatic cells
The human hippocampus and prefrontal cortex play critical roles in learning cognition
Musical abilities such as recognising music and singing performance serve means for communication are instruments in sexual selection. Specific regions of the brain have been found to be activated by musical stimuli, but these rarely extended discovery genes molecules associated with ability.A total 1008 individuals from 73 families were enrolled a pitch-production accuracy test was applied determine ability. To identify genetic loci variants that contribute ability, we conducted...
Effective tumor regression has been observed with chimeric antigen receptor (CAR) T cells; however, the development of an affordable, safe, and effective CAR-T cell treatment remains a challenge. One major obstacles is that suboptimal genetic modification cells reduces their yield antitumor activity, necessitating next-generation engineering approach. In this study, we developed nonviral nanoengineering system allows highly efficient delivery diverse functional nanomaterials into primary...
Chromatin conformation reorganization is emerging as an important layer of regulation for gene expression and lineage specification. Yet, how lineage-specific transcription factors contribute to the establishment cell type-specific 3D chromatin architecture in immune cells remains unclear, especially late stages T subset differentiation maturation. Regulatory (Treg) are mainly generated thymus a subpopulation specializing suppressing excessive responses. Here, by comprehensively mapping...
ABSTRACT Single-cell technologies enable measure of unique cellular signatures, but are typically limited to a single modality. Computational approaches allow integration diverse single-cell datasets, their efficacy is difficult validate in the absence authentic multi-omic measurements. To comprehensively assess molecular phenotypes cells tissues, we devised single-nucleus methylCytosine, Chromatin accessibility and Transcriptome sequencing (snmC2T-seq) applied it post-mortem human frontal...
Hypoxia enhances histone methylation by inhibiting oxygen- and α-ketoglutarate-dependent demethylases, resulting in increased methylated histones. This study reveals how hypoxia-induced affects clipping the reorganization of heterochromatin into senescence-associated foci (SAHF) during oncogene-induced senescence (OIS) IMR90 human fibroblasts. Notably, using top-down proteomics, we discovered specific cleavage sites targeted Cathepsin L (CTSL) H3, H2B H4 Raf activation, identifying novel H4....
Integrin-mediated cell-matrix interactions play an important role in osteogenesis. Here, we constructed a novel osteoinductive fibronectin matrix protein (oFN) for bone tissue engineering, designed to combine the integrin-binding modules from (iFN) and strong growth factor, morphogenetic protein-2. Compared with iFN, purified oFN caused significant increase cell adhesion osteogenic differentiation of pre-osteoblast MC3T3-E1 cells (p < 0.05).
Abstract The human frontal cortex and hippocampus play critical roles in learning cognition. We investigated the epigenomic 3D chromatin conformational reorganization during development of hippocampus, using more than 53,000 joint single-nucleus profiles conformation DNA methylation (sn-m3C-seq). remodeling predominantly occurs late-gestational to early-infant is temporally separated from dynamics. Neurons have a unique Domain-Dominant that different Compartment-Dominant glial cells...
Chromatin conformation reorganization is emerging as an important layer of regulation for gene expression and lineage specification. Yet, how lineage-specific transcription factors contribute to the establishment cell type-specific 3D chromatin architecture in immune cells remains unclear, especially late stages T subset differentiation maturation. Regulatory (Treg) are mainly generated thymus a subpopulation specializing suppressing excessive responses. Here, by comprehensively mapping...
Obesity is a risk factor associated with numerous disorders, such as type 2 diabetes, hypertension, dyslipidemia and coronary heart disease. In this study, we investigated the inhibitory effects of Pericarpium zanthoxyli extract (PZE) on adipocytic differentiation OP9 cells. During adipocyte differentiation, cells were treated 0, 10 20 µg/ml PZE at various time intervals, followed by examination lipid droplet formation mRNA expression adipogenesis-related genes. The during early period (days...