A5100393952- RNA modifications and cancer
- Epigenetics and DNA Methylation
- PARP inhibition in cancer therapy
- Growth Hormone and Insulin-like Growth Factors
- Cholesterol and Lipid Metabolism
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Protein Degradation and Inhibitors
- Vitamin C and Antioxidants Research
- Histone Deacetylase Inhibitors Research
- Cancer-related gene regulation
- Estrogen and related hormone effects
- Peptidase Inhibition and Analysis
- Peroxisome Proliferator-Activated Receptors
- Genomics and Chromatin Dynamics
- Circular RNAs in diseases
- Autophagy in Disease and Therapy
- Metabolism, Diabetes, and Cancer
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Folate and B Vitamins Research
- Fibroblast Growth Factor Research
- Plant Genetic and Mutation Studies
- Retinal Development and Disorders
- Scoliosis diagnosis and treatment
- Photosynthetic Processes and Mechanisms
Zhejiang University
2025
He Eye Hospital
2024
East China University of Science and Technology
2023
Second Military Medical University
2023
Liaoning Provincial People's Hospital
2022
Northwest A&F University
2020-2021
State Council of the People's Republic of China
2021
The University of Texas MD Anderson Cancer Center
2020
Agency for Science, Technology and Research
2008-2017
Singapore Immunology Network
2017
Polycomb-repressive complex 2 (PRC2)-mediated histone methylation plays an important role in aberrant cancer gene silencing and is a potential target for therapy. Here we show that S-adenosylhomocysteine hydrolase inhibitor 3-Deazaneplanocin A (DZNep) induces efficient apoptotic cell death cells but not normal cells. We found DZNep effectively depleted cellular levels of PRC2 components EZH2, SUZ12, EED inhibited associated H3 Lys 27 (but 9 methylation). By integrating RNA interference...
Inhibitors of histone deacetylases (HDACIs) are a new generation anticancer agents that selectively kill tumor cells. However, the molecular basis for their selectivity is not well understood. We investigated effects HDACIs on oncogenic Rb-E2F1 pathway, which frequently deregulated in human cancers. Here, we report cancer cells with elevated E2F1 activity, caused either by enforced expression, or E1A oncogene highly susceptible to HDACI-induced cell death. This E2F1-mediated apoptosis...
Breast cancer is susceptible to Candida infections, and candidiasis has an enhancing effect on the progression metastasis of tumor. concurrent represent a significant challenge in clinical therapy. Herein, series novel small molecule inhibitors simultaneously targeting bromodomain extra-terminal (BET) histone deacetylase (HDAC) were designed for combinational treatment breast resistant albicans infections. Among them, compounds 13c 17b exhibited excellent balanced inhibitory activity against...
Over the past several decades, invasive fungal infections, especially candidiasis, have caused dramatic morbidity and mortality due to ineffective antifungal drugs severe drug resistance. Herein, new BRD4–histone deacetylase (HDAC) inhibitors were designed restore susceptibility of Candida albicans (C. albicans) fluconazole (FLC). Interestingly, compounds showed excellent selectivity against HDACs. In particular, compound B2 synergistic effect with FLC resistant C. (FICI = 0.063) high HDACs...
Expression quantitative trait loci (eQTL) databases represent a valuable resource to link disease-associated SNPs specific candidate genes whose gene expression is significantly modulated by the SNP under investigation. We previously identified signal inhibitory receptor on leukocytes-1 (SIRL-1) as powerful regulator of human innate immune cell function. While it constitutively high expressed neutrophils, monocytes SIRL-1 surface varies strongly between individuals. The underlying mechanism...
Abstract Epigenetic regulation of gene transcription has been shown to coordinate with nutrient availability, yet the mechanisms underlying this coordination remain incompletely understood. Here, we show that glucose starvation suppresses histone 2A K119 monoubiquitination (H2Aub), a modification correlates repression. Glucose suppressed H2Aub levels independently energy stress–mediated AMP-activated protein kinase activation and possibly through NADPH depletion subsequent inhibition BMI1,...
The 5'-untranslated region of the human prolactin receptor (hPRLR) gene contains two alternative first exons, hE1(3), counterpart rat and mouse E1(3) a novel type exon termed hE1N, also common non-coding 2 third containing translation initiation codon. hE1(3) was localized approximately 800 bp 5' from hE1N in genome. distinct exons are expressed breast tissue, cancer cells, gonads liver. Overall, transcript is prevalent most tissues. coding comprises eight (exon 3-10), which 10 encodes...
The expression of the prolactin receptor is under control two putative tissue-specific (PI, gonads; PII, liver) and one common (PIII) promoters (Hu, Z. Z., Zhuang, L., Dufau, M. L. (1996) J. Biol. Chem.271, 10242–10246). three promoter regions were co-localized to rat chromosomal locus 2ql6, in order 5′-PIII-PI-PII-3′. To investigate mechanisms gonad-specific utilization PI, domain, regulatory cis-elements, trans-factors identified gonadal cells. domain localized 152-base pair 5′...
Three promoters are operative in the rat prolactin receptor gene as follows: promoter I (PI) and II (PII) specific for gonads liver, respectively, III (PIII) is common to several tissues. To investigate mechanisms controlling activity of III, its regulatory elements transcription factors were characterized gonadal non-gonadal cells. The TATA-less PIII domain was localized region −437 −179 (ATG +1) containing 5′-flanking part non-coding first exon. Within domain, a functional...
Transcription of the prolactin receptor (PRLR) is under control multiple promoters. Following recent demonstration human non-coding exon 1, hE1(N) (hE1(N1)) and generic 1 hE1(3), we have identified their promoters characterized four other novel exons (hE1(N2-5)) that are alternatively spliced to a common 2 in tissues breast cancer cells. Genomic regions containing these exons, 5'-flanking intronic sequences, were determined order was established chromosome 5p14-13. Promoters utilized...
FoxO1 is a crucial transcription factor involved in lipid metabolism mouse liver through repressing key regulator of lipogenesis, sterol regulatory element binding protein 1 (SREBP1). However, it remains elusive whether plays roles the regulation fatty acid during lactation dairy goats. In this study, we aim to investigate function goat mammary epithelial cells (GMECs). We found that expression significantly upregulated compared with dry period. knockdown enhanced genes related de novo...
Several reports suggest that epigenetic therapy may be a potential method for treating epilepsy, and circular RNAs (circRNAs) play important roles in mediating the mechanisms associated with epilepsy; however, currently there are no effective treatment methods to prevent progression of epileptogenesis. The circRNA serine/arginine repetitive matrix 4 (circSRRM4) was found exert regulatory effects temporal lobe epilepsy (TLE); involved still unknown.To elucidate molecular mechanism circSRRM4,...
Abstract DNA methylation, which can affect the expression level of genes, is one most vital epigenetic modifications in mammals. Fibroblast growth factor receptor 1 ( FGFR1 ) plays an important role muscle development; however, methylation promoter has not been studied to date cattle. Our study focused on and its effect bovine myoblast proliferation differentiation. We identified core by using luciferase reporter assays; we then reverse transcription quantitative polymerase chain reaction,...
Adeno-associated virus (AAV) vectors have been widely used in therapy to treat hereditary retinal diseases. But its transduction efficiency by intravitreal injection still needs be improved. In this study, we investigated the of AAV-DJ (K137R)-GFP different cells normal mice, as well effection (K137R)-Rs1 on function and structure Rs1-KO mice. The demonstrated that vector transduced all layers retina, including inner nuclear layer photoreceptor layer. found 3 months post-injection improved...
Supplementary Figure 4 from Ribosomal Protein S27-like, a p53-Inducible Modulator of Cell Fate in Response to Genotoxic Stress
Supplementary Figure 5 from Ribosomal Protein S27-like, a p53-Inducible Modulator of Cell Fate in Response to Genotoxic Stress