A5019867019- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Insect symbiosis and bacterial influences
- Chromosomal and Genetic Variations
- RNA and protein synthesis mechanisms
- Hepatitis B Virus Studies
- Acute Myeloid Leukemia Research
- Acute Lymphoblastic Leukemia research
- Insect Resistance and Genetics
- Cancer-related gene regulation
- Genomics and Phylogenetic Studies
- Protein Degradation and Inhibitors
- Sex and Gender in Healthcare
- Viral gastroenteritis research and epidemiology
- Genetics, Aging, and Longevity in Model Organisms
- Hepatitis C virus research
- Ubiquitin and proteasome pathways
- Genetic Syndromes and Imprinting
- Microbial Metabolic Engineering and Bioproduction
- Cancer-related molecular mechanisms research
- Protein Structure and Dynamics
Institute of Molecular Biology
2020-2025
Max Planck Institute of Immunobiology and Epigenetics
2015-2021
Friedrich Miescher Institute
2009-2015
University of Basel
2012-2015
Justus-Liebig-Universität Gießen
1992-2001
Institute of Cancer Research
1997
Giessen School of Theology
1994
Universitätsklinikum Gießen und Marburg
1992
Universität Hamburg
1983
Epidemiological evidence has suggested that some pediatric leukemias may be initiated in utero and, for pairs of identical twins with concordant leukemia, this possibility been strongly endorsed by molecular studies clonality. Direct a prenatal origin can only derived prospective or retrospective detection leukemia-specific abnormalities fetal newborn samples. We report PCR-based method developed to scrutinize neonatal blood spots (Guthrie cards) the presence numerically infrequent leukemic...
Abstract Structural resolution of protein interactions enables mechanistic and functional studies as well interpretation disease variants. However, structural data is still missing for most because we lack computational experimental tools at scale. This particularly true mediated by short linear motifs occurring in disordered regions proteins. We find that AlphaFold-Multimer predicts with high sensitivity but limited specificity structures domain-motif when using small fragments input....
Journal Article Plasmids of Human Strains Yersinia enterocolitica: Molecular Relatedness and Possible Importance for Pathogenesis Get access J. Heesemann, Heesemann From the Institute Medical Microbiology Immunology, University Hamburg, Federal Republic Germany Search other works by this author on: Oxford Academic PubMed Google Scholar C. Keller, Keller R. Morawa, Morawa N. Schmidt, Schmidt H. Siemens, Siemens Laufs The Infectious Diseases, Volume 147, Issue 1, January 1983, Pages 107–115,...
Genome rearrangements that occur during evolution impose major challenges on regulatory mechanisms rely three-dimensional genome architecture. Here, we developed a scaffolding algorithm and generated chromosome-length assemblies from Hi-C data for studying topology in three distantly related Drosophila species. We observe extensive shuffling between these species with one synteny breakpoint after approximately every six genes. A/B compartments, set of large gene-dense topologically...
Abstract In mammals, X-chromosomal genes are expressed from a single copy since males (XY) possess X chromosome, while females (XX) undergo inactivation. To compensate for this reduction in dosage compared with two active copies of autosomes, it has been proposed that the chromosome exhibit compensation. However, existence and mechanisms X-to-autosome compensation still under debate. Here we show transcripts have fewer m 6 A modifications more stable than their autosomal counterparts. Acute...
Abstract Haploinsufficiency and aneuploidy are two phenomena, where gene dosage alterations cause severe defects ultimately resulting in developmental failures disease. One remarkable exception is the X chromosome, copy number differences between sexes buffered by compensation systems. In Drosophila , Male-Specific Lethal complex (MSLc) mediates upregulation of single male chromosome. The evolutionary origin conservation this process orchestrated MSL2, only male-specific protein within fly...
The Anopheles mosquito is one of thousands species in which sex differences play a central part their biology, as only females need blood meal to produce eggs. Sex differentiation regulated by chromosomes, but presence creates dosage imbalance between males (XY) and (XX). Dosage compensation (DC) can re-equilibrate the expression chromosomal genes. However, because DC mechanisms have been fully characterized few model organisms, key questions about its evolutionary diversity functional...
A nested polymerase chain reaction (PCR) protocol was developed for rapid genotyping of hepatitis B virus (HBV). During the first PCR round, a universal HBV primer pair used to amplify entire pre-S region genome. Within region, many nucleotide exchanges are observed. These partly correlated serological surface antigen subtypes. Five additional subtype-specific primers were selected from that which, together with two non-group-specific primers, generated specific combinations four DNA...
Abstract Insects display exceptional phenotypic plasticity, which can be mediated by epigenetic modifications, including CpG methylation and histone modifications. In vertebrates, both are interlinked is associated with gene repression. However, little known about these regulatory systems in invertebrates, where mainly restricted to bodies of transcriptionally active genes. A widely conserved mechanism involves the co-transcriptional deposition H3K36 trimethylation targeted unmethylated CpGs...
Sex chromosomes impact chromatin organization and histone modification dynamics differently between males females, particularly those involved in dosage compensation (DC). The stability of DC mechanisms may shape sex-specific phenotypes traits such as lifespan. However, the tissue- age-dependent variations well evolutionary diversity are incompletely understood. Here, we investigate occurrence H4 lysine 16 acetylation (H4K16ac), previously known for its role sex chromosome male-heterogametic...
ABSTRACT Insects display exceptional phenotypic plasticity, which can be mediated by epigenetic modifications, including CpG methylation and histone modifications. In vertebrates, both are interlinked is associated with gene repression. However, little known about these regulatory systems in invertebrates, where mainly restricted to bodies of transcriptionally active genes. A widely conserved mechanism involves the co‐transcriptional deposition H3K36 trimethylation targeted unmethylated CpGs...
Abstract The MLL ( HRX, ALL‐1 HTRX ) gene at chromosome band 11q23 frequently is rearranged in acute lymphoblastic and myeloblastic leukemia. To date, more than 40 different abnormalities have been described on the cytogenetic level, least 25 of respective fusion partner genes are cloned. vast majority reciprocal translocations generate a chimeric 5′‐ /partner‐3′ derivative 11q23. In this work, we report unique ins(X;11)(q24;q23) an infant with myeloid leukemia (AML‐M2) that fuses human...
Self-renewal and differentiation of hematopoietic stem cells (HSCs) are orchestrated by the combinatorial action transcription factors epigenetic regulators. Here, we have explored mechanism which histone H4 lysine 16 acetyltransferase MOF regulates erythropoiesis. Single-cell RNA sequencing chromatin immunoprecipitation uncovered that influences erythroid trajectory dynamic recruitment to its haploinsufficiency causes accumulation a transient HSC population. A regulatory network consisting...
The fission yeast Cid14 protein belongs to a family of noncanonical poly(A) polymerases which have been implicated in broad range biological functions. Here we describe an extensive protein–protein interaction network and its biochemical dissection. most stably interacts with the zinc-knuckle Air1 form Cid14–Air1 complex (CAC). Providing link ribosomal RNA processing, sediments 60S subunits copurifies assembly factors. In contrast, no physical chromatin has identified, although gene...
Abstract Proteins of the conserved HP 1 family are elementary components heterochromatin and generally assumed to play a central role in creation rigid, densely packed heterochromatic network that is inaccessible transcription machinery. Here, we demonstrate fission yeast protein Swi6 exists as single highly dynamic population rapidly exchanges cis trans between different regions. Binding methylated H3K9 or RNA decelerates mobility. We further show largely dispensable maintenance domains. In...