A5054456948- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Genomics and Chromatin Dynamics
- COVID-19 Clinical Research Studies
- Biosensors and Analytical Detection
- Mosquito-borne diseases and control
- Plant Virus Research Studies
- Viral Infections and Vectors
- COVID-19 Impact on Reproduction
- Vector-Borne Animal Diseases
- Advanced biosensing and bioanalysis techniques
- COVID-19 diagnosis using AI
- RNA and protein synthesis mechanisms
- Animal Disease Management and Epidemiology
- Chromosomal and Genetic Variations
- COVID-19 epidemiological studies
- RNA modifications and cancer
- Gestational Diabetes Research and Management
- CRISPR and Genetic Engineering
- Pregnancy and preeclampsia studies
- HIV Research and Treatment
- Plant Molecular Biology Research
- Immune responses and vaccinations
- RNA Research and Splicing
- Viral gastroenteritis research and epidemiology
UK Health Security Agency
2024-2025
London School of Hygiene & Tropical Medicine
2020-2024
Barts Health NHS Trust
2021-2022
Royal London Hospital
2021-2022
Wellcome Centre for Cell Biology
2016-2017
University of Edinburgh
2016-2017
Friedrich Miescher Institute
2013-2015
University of Basel
2013
The University of Texas Medical Branch at Galveston
2009
Abstract Background The UK’s National Health Service Test and Trace (NHSTT) program aimed to provide the most effective accessible SARS-CoV-2 testing approach possible. Early user feedback indicated that there were accessibility issues associated with throat swabbing. We report results of service evaluations performed by NHSTT assess effectiveness acceptance swabbing approaches, as well qualitative findings experiences from research reports, surveys, incident reports. Our intent is present...
The advent of lateral flow devices (LFDs) for SARS-CoV-2 detection enabled widespread use rapid self-tests during the pandemic. While self-testing using LFDs is now common, whether provides comparable performance to professional testing was a key question that remained important pandemic planning.
Scientific Report3 March 2016Open Access Transparent process Centromere localization and function of Mis18 requires Yippee-like domain-mediated oligomerization Lakxmi Subramanian Wellcome Trust Centre for Cell Biology, Institute University Edinburgh, UK Search more papers by this author Bethan Medina-Pritchard Rachael Barton Frances Spiller Raghavendran Kulasegaran-Shylini Guoda Radaviciute Robin C Allshire A Arockia Jeyaprakash Corresponding Author Information Subramanian1,‡,...
Background SARS-CoV-2 lineage B.1.1.7 has been associated with an increased rate of transmission and disease severity among subjects testing positive in the community. Its impact on hospitalised patients is less well documented. Methods We collected viral sequences clinical data admitted hospital-onset COVID-19 infections (HOCIs), sampled 16 November 2020 to 10 January 2021, from eight hospitals participating COG-UK-HOCI study. Associations between variant outcomes all-cause mortality...
Background Rapid identification of individuals with acute respiratory infections is crucial for preventing nosocomial infections. For rapid diagnosis, especially in EDs, lateral flow devices (LFDs) are a convenient, inexpensive option turnaround. Several ‘multiplex’ LFDs (M-LFDs) now exist, testing multiple pathogens from single swab sample. We evaluated the real-world performance M-LFD versus PCR detecting influenza A, B and SARS-CoV-2) ED setting. Methods After preliminary evaluation an...
Venezuelan equine encephalitis virus (VEEV) is one of the most pathogenic members Alphavirus genus in Togaviridae family. The pathogenesis this depends strongly on sequences structural proteins and mutations RNA promoter encoded by 5' untranslated region (5'UTR) viral genome. In study, we performed a detailed investigation functional elements 5'-terminal analyzed effect multiple introduced into VEEV 5'UTR replication. results study demonstrate that replication determined two synergistically...
Abstract Proteins of the conserved HP 1 family are elementary components heterochromatin and generally assumed to play a central role in creation rigid, densely packed heterochromatic network that is inaccessible transcription machinery. Here, we demonstrate fission yeast protein Swi6 exists as single highly dynamic population rapidly exchanges cis trans between different regions. Binding methylated H3K9 or RNA decelerates mobility. We further show largely dispensable maintenance domains. In...
We investigated if performing two lateral flow device (LFD) tests, LFD2 immediately after LFD1, could improve diagnostic sensitivity or specificity for detecting severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) antigen.
ObjectivesRecently emerging SARS-CoV-2 variants have been associated with an increased rate of transmission within the community. We sought to determine whether this also resulted in hospitals.MethodsWe collected viral sequences and epidemiological data patients community healthcare infections, sampled from 16th November 2020 10th January 2021, nine hospitals participating COG-UK HOCI study. Outbreaks were identified using ward information, lineage pairwise genetic differences between...
Abstract How do segmented RNA viruses correctly recruit their genome has yet to be clarified. Bluetongue virus is a double-stranded with 10 segments of different sizes, but it assembles its in single-stranded form through series specific RNA–RNA interactions prior packaging. In this study, we determined the structure each BTV transcript, individually and combinations, using 2′-hydroxyl acylation analysed by primer extension mutational profiling (SHAPE-MaP). SHAPE-MaP identified structural...
We observed an increased frequency of massive perivillous fibrin deposition (MPFD) during the second coronavirus disease 2019 (COVID-19) pandemic wave dominated by Alpha variant severe acute respiratory syndrome 2 (SARS-CoV-2). MPFD associated with 100% reverse transcription polymerase chain reaction (RT-PCR) positivity for SARS-CoV-2 and detection immunohistochemistry. The was identified in all placentas that could be sequenced.
Background The NHS Test and Trace (NHSTT) programme was established in May 2020 England to deliver SARS-CoV-2 testing contact tracing order identify infected individuals reduce COVID-19 spread. To further control transmission, people identified as contacts were asked self-isolate for 10 days test only if they became symptomatic. From March 2021, eligibility criteria PCR expanded include asymptomatic of confirmed cases. Aim analyse patterns before after the change guidance assess impact on...
Abstract To detect SARS-CoV-2 amongst asymptomatic care home staff in England, a dual-technology weekly testing regime was introduced on 23 December 2020. A lateral flow device (LFD) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) test were taken the same day (day 0) midweek LFD three to four days later. We evaluated effectiveness of using between 2020 April 2021. Viral concentrations derived from qRT-PCR used determine probable stage infection likely level...
Abstract Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) lineage B.1.1.7 has been associated with an increased rate of transmission and disease severity among subjects testing positive in the community. Its impact on hospitalised patients is less well documented. Methods We collected viral sequences clinical data admitted SARS-CoV-2 hospital-onset COVID-19 infections (HOCIs), sampled 16/11/2020 - 10/01/2021, from eight hospitals participating COG-UK-HOCI study....
SUMMARY The underlying hallmark of centromeres is the presence specialized nucleosomes in which histone H3 replaced by CENP-A. events that mediate installation CENP-A place remain poorly characterized. H2A.Z linked to transcriptional competence and associates with mammalian centromeres. We find Pht1 Swr1 complex are enriched fission yeast Cnp1 chromatin. Our analysis shows Swr1, Msc1 required maintain chromatin integrity. Cell cycle analyses demonstrate deposited S phase, coincident...
Background: Recently emerging SARS-CoV-2 variants have been associated with an increased rate of transmission within the community. Little is known about impact their infectivity has on hospitals.Methods: We collected viral sequences and epidemiological data patients community healthcare infections, sampled from 16th November 2020 to 10th January 2021, nine hospitals participating in COG-UK HOCI study. Outbreaks were identified using ward information, lineage pairwise genetic differences...