A5003041884- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- SARS-CoV-2 detection and testing
- Virus-based gene therapy research
- Biosensors and Analytical Detection
- Microbial Natural Products and Biosynthesis
- RNA and protein synthesis mechanisms
- Fungal and yeast genetics research
Rice University
2021-2022
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems have recently received notable attention for their applications in nucleic acid detection. Despite many attempts, the majority of current CRISPR-based biosensors infectious respiratory disease diagnostic still require target preamplifications. This study reports a new biosensor amplification-free detection via harnessing trans-cleavage mechanism Cas13a and ultrasensitive graphene...
Genome mining of cryptic natural products (NPs) remains challenging, especially in filamentous fungi, owing to their complex genetic regulation. Increasing evidence indicates that several epigenetic modifications often act cooperatively control fungal gene transcription, yet the ability predictably manipulate multiple genes simultaneously is still largely limited. Here, we developed a multiplex base-editing (MBE) platform significantly improves capability and throughput genome manipulation,...
Base editors (BEs) hold great potential for medical applications of gene therapy. However, high precision base editing requires BEs that can discriminate between the target and multiple bystander bases within a narrow active window (4 - 10 nucleotides). Here, to assist in design these optimized editors, we propose discrete-state stochastic approach build an analytical model explicitly evaluates probabilities bystanders. Combined with all-atom molecular dynamic simulations, our reproduces...
Abstract The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated (Cas) systems have recently received notable attention for their applications in nucleic acid detection. Despite many attempts, the majority of current CRISPR‐based biosensors infectious respiratory disease diagnostic still require target preamplifications. This study reports a new biosensor amplification‐free detection via harnessing trans‐cleavage mechanism Cas13a and ultrasensitive graphene...
Der Nachweis von Nukleinsäuren spielt eine wichtige Rolle in der medizinischen Diagnostik, Umweltüberwachung und Lebensmittelsicherheit. In ihrem Forschungsartikel (e202203826) entwickelten Xue Gao, Yi Zhang Mitarbeiter einen neuen Biosensor für den amplifikationsfreien Nukleinsäurenachweis, indem sie trans-Spaltungsmechanismus Cas13a ultrasensitive Graphen-Feldeffekttransistoren (gFETs) nutzten. Die Abbildung zeigt die Cas13a-vermittelte RNA-trans-Spaltung auf gFET-Oberfläche...